Blood samples were collected in the morning after an overnight fast. Plasma sLOX-1 concentrations were measured using a sandwich enzyme-linked immunosorbent assay (ELISA) with two specific monoclonal antibodies against sLOX-1, with recombinant sLOX-1 as the assay standard. In our laboratory, intra-assay and inter-assay CVs for blood sLOX-1 concentration were 1.6–5.2% and 3.9–7.7%, respectively.(5 (link))Serum concentrations of low- and high-density lipoprotein cholesterol (LDL and HDL, respectively) and triglycerides were measured by using commercial enzymatic kits (Cholestest®LDL; Sekisui Medical, Tokyo, Japan: Determiner L HDL-C or MetaboLead HDL-C; Kyowa Medex, Tokyo, Japan: and Determiner C-TG; Kyowa Medex) and an autoanalyzer (JCA-BM 8060; JEOL, Tokyo, Japan). Plasma glucose concentrations and haemoglobin A1c were determined by using a enzymatic kit (L-type Wako Glu2; Wako Pure Chem. Ind., Ltd., Osaka, Japan), a latex agglutination kit (Determiner L HbA1c, Kyowa Medex), and an autoanalyzer (JCA-BM 9130; JEOL). Serum insulin levels were assessed by an electrochemiluminescence immunoassay by using an analyzer (E170; Roche Diagnostics K.K., Tokyo, Japan) and the designated regents.
Jca bm9130
Manufactured by JEOL
Sourced in Japan
The JCA-BM9130 is a compact and versatile benchtop scanning electron microscope (SEM) designed for laboratory use. It provides high-resolution imaging capabilities for a wide range of materials and samples. The core function of the JCA-BM9130 is to capture detailed surface images and topographical information of samples at the micro- and nano-scale.
Automatically generated - may contain errors
Lab products found in correlation
Jca bm8060, by JEOL (3 mentions)
Xe 2100, by Sysmex (2 mentions)
Jca bm9030, by JEOL (2 mentions)
Cholestest ldl, by Sekisui (2 mentions)
Labospect008α, by Hitachi (2 mentions)
Cobas 8000 analyzer series module e801, by Roche (1 mentions)
L type wako glu2, by Fujifilm (1 mentions)
Acquity uplc system, by Waters Corporation (1 mentions)
Architect i2000sr, by Abbott (1 mentions)
Jca bm6050, by JEOL (1 mentions)
Jca zs050, by JEOL (1 mentions)
Accuras auto zn, by Shino-Test (1 mentions)
Quick auto neo cu, by Shino-Test (1 mentions)
Au5800, by Beckman Coulter (1 mentions)
10 protocols using jca bm9130
1
Measuring Blood Biomarkers for Cardiovascular Risk
2
Serum Biomarker Determination Protocol
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Serum GH levels were determined by Electro Chemiluminescence Immunoassay using Cobas 8000 analyzer series module e801 (Roche Diagnostics, Mannheim, Germany; %CV of intra-assay variability <15%). The serum glucose level was determined by hexokinase enzymatic method (L-type Wako Glu2; Wako Pure Chemical Industries, Osaka, Japan) using LABOSPECT 008 α (HITACHI, Tokyo, Japan) and JCA-BM8060 (JEOL, Tokyo, Japan). The lactate level was determined by enzymatic method (Deteminer LA; Minaris Medical, Tokyo, Japan) using JCA-BM9130 (JEOL, Tokyo, Japan). Serum insulin levels were determined by Chemiluminescent Immunoassay using Architect i2000 SR (Abbott, Osaka, Japan). Plasma amino acids fractionation was determined by High-Pressure Liquid Chromatography using a Modification of the 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate (AQC) Method on a Waters Acquity UPLC system. GH, glucose, insulin, amino acids, and lactate levels were analyzed by the LSI Medience Corporation (Kyoto, Japan). Acylated ghrelin levels were measured using an Active Ghrelin ELISA Kit (# 97751, LSI Medience, Tokyo, Japan).
3
Enzymatic Blood Parameter Analyses
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Blood samples were centrifuged at 1,200 g for 10 min at room temperature to separate the serum components. Measurement of blood parameters was outsourced to LSI Medience Corporation (Tokyo, Japan).
HbA1c level in whole blood was measured enzymatically using a clinical chemistry analyser (JCA-BM9130, JEOL Ltd., Tokyo, Japan). PG, GA, TAG, TC, LDL-C, and HDL-C levels were determined enzymatically, and serum insulin
levels were determined using a chemiluminescent immunoassay. Non-HDL-C levels were determined using the following equation: non-HDL-C = TC – HDL-C.
HbA1c level in whole blood was measured enzymatically using a clinical chemistry analyser (JCA-BM9130, JEOL Ltd., Tokyo, Japan). PG, GA, TAG, TC, LDL-C, and HDL-C levels were determined enzymatically, and serum insulin
levels were determined using a chemiluminescent immunoassay. Non-HDL-C levels were determined using the following equation: non-HDL-C = TC – HDL-C.
4
Comprehensive Metabolic and Hematological Evaluation
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The levels of serum lipids, aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatinine, and uric acid were evaluated using reflection spectrometry with an Auto-analyzer 7700 (Hitachi, Tokyo, Japan). Serum glucose was evaluated using a biochemical automatic analyzer, JCA-BM9130 (JEOL, Tokyo, Japan). Red blood cells, hemoglobin, hematocrit, mean corpuscular volume (MCV), platelets, and white blood cells were measured using a hematology analyzer, the XE-2100 (Sysmex, Kobe, Japan).
5
Comprehensive Blood and Metabolic Panel
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White blood cell count, red blood cell count, hemoglobin, hematocrit, platelet count, total protein, albumin, total bilirubin, AST, ALT, LDH, γ-GTP, ALP, urea nitrogen, uric acid, creatinine, total cholesterol, HDL-Cholesterol, LDL cholesterol, triglyceride, sodium, potassium, chloride, HbA1c, and fasting blood glucose were measured. White blood cells, red blood cells, hemoglobin, hematocrit, and platelets were measured using Sysmex XE-2100 automated hematology analyzer (Sysmex, Kobe, Japan). Total protein, albumin, total bilirubin, AST, ALT, LDH, γ-GTP, ALP, urea nitrogen, uric acid, creatinine, total cholesterol, HDL-cholesterol, LDL cholesterol, triglyceride, sodium, potassium, and chloride were measured with LABOSPECT 008α (Hitachi High-Tech, Tokyo, Japan) and JCA-BM8060 (JEOL Ltd., Tokyo, Japan). HbA1c and fasting blood glucose levels were measured with JCA-BM9030 and JCA-BM9130 (JEOL Ltd., Tokyo, Japan).
6
Comprehensive Blood and Biochemistry Analysis
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Blood and biochemical parameters were determined using an automatic Sysmex XE-2100 differential analyzer (LSI Medience, Tokyo, Japan) and a LABOSPECT 008 α analyzer (Hitachi High-Technologies Corporation, Tokyo, Japan). Blood sugar was determined using the GK-G6PD enzymatic method (JCA-BM9130, JCA-BM9030, JEOL Ltd., Tokyo, Japan). Plasma human atrial natriuretic peptide (hANP) concentrations were determined by the chemiluminescent enzyme immunoassay method.
7
Automated Hepcidin-25 Quantification Protocol
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Serum hepcidin-25 was measured using a hepcidin-25 measurement reagent (FUJIFILM Wako Pure Chemical Corporation) and automated biochemistry analyzers (JCA-BM6050, JCA-BM9130, JEOL Ltd., Tokyo, Japan; TBA-c16000, Canon Medical Systems Co., Ltd., Tochigi, Japan). Then, Reagent 1 (60 μL or 120 μL) and a 2-fold diluted sample or undiluted sample (1.6 μL) were mixed and reacted at 37 °C for about 5 min. Subsequently, Reagent 2 (20 μL or 40 μL) was added and reacted at 37 °C for about 5 min. The hepcidin-25 in the sample reacts with the antibody-sensitized latex particles, causing turbidity. Since the degree of turbidity is proportional to the hepcidin-25 concentration in the sample, the hepcidin-25 concentration in the sample can be obtained by measuring the change in turbidity near the wavelength of 805 nm.
8
Cardiac Function Assessment via RHC
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We retrospectively reviewed the medical records of all consecutive patients who underwent RHC to assess cardiac function in Showa University Hospital between January 1, 2012, and March 31, 2018. This study was approved by the ethics committee of Showa University School of Medicine.
The details about patient history, physical examination, blood investigations, and echocardiography were evaluated. Patients with the following diseases that could affect serum tPAI-1 and TM levels were excluded from the study: (1) those on hemodialysis [11 (link)]; (2) those currently under treatment for malignant neoplasms; (3) those with hepatic failure [12 (link), 13 (link)]; (4) those with congestive heart failure (CHF) NYHA grade III or more.
Blood samples were collected from patients at admission before RHC was performed. Fibrinolytic and coagulation marker levels, including tPAI-1, TM, D-dimer, thrombin-antithrombin complex (TAT), plasmin-α2 plasmin inhibitor complex (PIC), and prothrombin fragment 1 + 2 (PTF 1 + 2) were also measured. The tPAI-1 level was measured using the latex agglutination test (JCA-BM9130, JEOL Ltd., Tokyo, Japan). The TM level was measured using an enzyme-linked immunoassay (ELISA) kit, AP-X (Kyowa Medex Co Ltd., Tokyo, Japan) [14 (link)].
The details about patient history, physical examination, blood investigations, and echocardiography were evaluated. Patients with the following diseases that could affect serum tPAI-1 and TM levels were excluded from the study: (1) those on hemodialysis [11 (link)]; (2) those currently under treatment for malignant neoplasms; (3) those with hepatic failure [12 (link), 13 (link)]; (4) those with congestive heart failure (CHF) NYHA grade III or more.
Blood samples were collected from patients at admission before RHC was performed. Fibrinolytic and coagulation marker levels, including tPAI-1, TM, D-dimer, thrombin-antithrombin complex (TAT), plasmin-α2 plasmin inhibitor complex (PIC), and prothrombin fragment 1 + 2 (PTF 1 + 2) were also measured. The tPAI-1 level was measured using the latex agglutination test (JCA-BM9130, JEOL Ltd., Tokyo, Japan). The TM level was measured using an enzyme-linked immunoassay (ELISA) kit, AP-X (Kyowa Medex Co Ltd., Tokyo, Japan) [14 (link)].
9
Serum Zinc and Copper Levels in HD Patients
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Blood samples were collected before the first HD session of the week in which monitoring for this study was planned. Serum zinc levels were measured using automatic enzyme immunoassay device JCA-ZS050 (JEOL, Tokyo, Japan) with Accuras Auto Zn (Shino-Test Corporation, Sagamihara, Japan). Serum copper levels were measured using automatic enzyme immunoassay device JCA-BM9130 (JEOL, Tokyo, Japan) with Quick Auto Neo Cu (Shino-Test Corporation, Sagamihara, Japan).
10
Estimating Kidney Function with Multivariate Regression
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Multiple linear regression analysis was performed using mCCr values as dependent variables, and eCCr(Enz + 0.2) values, serum albumin values, triceps skinfold thickness (TSF), arm muscle area (AMA), skeletal muscle mass (SMM), hemoglobin values, and body fat mass (BFM) as independent variables. Multicollinearity was confirmed not to occur between independent variables based on variance inflation factor (VIF) values. Parameters having substantial impact on the dependent variable (mCCr) based on the absolute value of the standard partial regression coefficient (β) were selected and new estimation formulas (C) and (D) were created in the results.
SCr and UCr values were determined using the enzymatic method with an Aqua Auto-Kainos CRE-II reagent (Kainos Co., Tokyo, Japan). CysC values were measured using the gold colloid colorimetric method with the Nescoat GC Cystatin C Kit (Alfresa Pharma, Osaka, Japan). Several types of automatic analyzers were used as measurement devices (AU5800 (Beckman Coulter, Tokyo, Japan) ; JCA-BM 9130, JCA-BM 8030 (JEOL Ltd., Tokyo, Japan)).
SCr and UCr values were determined using the enzymatic method with an Aqua Auto-Kainos CRE-II reagent (Kainos Co., Tokyo, Japan). CysC values were measured using the gold colloid colorimetric method with the Nescoat GC Cystatin C Kit (Alfresa Pharma, Osaka, Japan). Several types of automatic analyzers were used as measurement devices (AU5800 (Beckman Coulter, Tokyo, Japan) ; JCA-BM 9130, JCA-BM 8030 (JEOL Ltd., Tokyo, Japan)).
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