Cd45ra percp cy5.5 hi100

Manufactured by BioLegend

CD45RA PerCP-Cy5.5 (HI100) is a fluorochrome-conjugated antibody that binds to the CD45RA surface antigen. CD45RA is a high molecular weight isoform of the CD45 protein expressed on the surface of certain types of T cells.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

PerCP-Cy5.5 (133 citations) CD45RA (HI100, (10 citations) CD45RA-PerCP-Cy5.5 (9 citations) PerCP-Cy5.5-CD45RA (2 citations) CD4 PE-Cy7 (SK3); CD8 PerCP-Cy5.5 (RPA-T8); CCR7 FITC (G043H7); CD45RA BV421 (HI100); HLA-DR PE (L243); CD38 BV605 (HIT2) (2 citations)

3 protocols using cd45ra percp cy5.5 hi100

Multiparameter Flow Cytometry of Hematopoietic Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single cell suspensions obtained from colony forming assays or hematopoietic organs from mice were surface stained with monoclonal antibodies. Antibodies used for analysis of human immature stem and progenitor cells from colony forming assays: CD34 PE-Cy7(581), CD38 APC (HIT2), CD10 PE/PeCy5/APC (HI10a), CD45RA PerCP-Cy5.5 (HI100), CD90 APC-Cy7 (5E10) (Biolegend). Antibodies for erythroid cells from colony assays and HEMA: CD117 PE-Cy7 (104D2), CD71 APC/APC-Cy7 (CY1G4) (Biolegend), CD235a BV421 (HIR2) (BD biosciences). Antibodies for myeloid cells from colony forming assays: CD34 PE-Cy7 (581), CD33 PE/PerCP-Cy5.5 (WM53), CD14 APC (M5E2), CD115 BV421 (g-4D2-1E4), CD15 PeCy5 (W6D3), CD66b PerCP-Cy5.5 (G10F5) (Biolegend). Antibodies for megakaryocyte populations: CD41 PerCP-Cy5.5 (HIP8), CD61 APC (VI-PL2) (Biolegend). Antibodies for overall human and human myeloid cells in xenograft assay: CD45 Biotin (HI30), CD34 PE-Cy7/APC-Cy7 (581), CD33 PE/PerCP-Cy5.5 (WM53), CD15 PeCy5 (W6D3). Antibodies for human B cells in xenograft assay: CD19 PE-Cy7 (HIB19), IgM APC-Cy7 (MHM-88), CD10 APC (HI10a) (Biolegend). Antibody to detect murine cells in xenograft assay: CD45 PE-Cy7/PerCP-Cy5.5 (30-F11) (Biolegend). Streptavidin APC/V450 (Biolegend) were used as secondary antibodies. BD LSRFortessa was used for flow cytometry. Analyses were performed using FlowJo.

Afreen S., Bohler S., Müller A., Demmerath E.M., Weiss J.M., Jutzi J.S., Schachtrup K., Kunze M, & Erlacher M. (2020). BCL-XL expression is essential for human erythropoiesis and engraftment of hematopoietic stem cells. Cell Death & Disease, 11(1), 8.

+ Open protocol

+ Expand

Comprehensive Vδ2 T Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

On day 14 of expansion, Vδ2 T cells were collected and washed in PBS and stained with Aqua viability dye (ThermoFisher). Next, cells were surface stained for the following antibodies: CD26 FITC (BA5b, Biolegend), CD45RA PerCpCy5.5 (HI100; Biolegend), CD160 Alexa Fluor 647 (BY55; BD Biosciences), TIGIT APC Fire 750 (A15153G; Biolegend), PD‐1 BV421 (EH12.2H7; Biolegend), NKG2D BV650 (1D11; BD Biosciences), CD27 BV786 (L128; BD Biosciences), CD94 BUV395 (HP‐3D9; BD Biosciences), Tim‐3 BUV737 (7D3; BD Biosciences), CD3 BUV805 (SK7; BD Biosciences), Vδ2 PE (B6; Biolegend) and 2B4 PE‐Dazzle (C1.7; Biolegend). After surface staining, cells were washed and resuspended in PBS containing 2% FCS before acquisition on a BD LSR Fortessa using BD FACS Diva.

Field K.R., Wragg K.M., Kent S.J., Lee W.S, & Juno J.A. (2024). γδ T cells mediate robust anti‐HIV functions during antiretroviral therapy regardless of immune checkpoint expression. Clinical & Translational Immunology, 13(2), e1486.

+ Open protocol

+ Expand

SARS-CoV-2 Spike Protein Peptide Stimulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cryopreserved PBMC were thawed and rested for 4 hours in RPMI-1640 supplemented with 10% FCS and penicillin-streptomycin (RF10). 2x10 6 PBMC were seeded per well in a 96-well U bottom plate and stimulated with 1ug/mL of a peptide pool covering the spike protein (PepTivator SARS-CoV-2 Prot_S Complete) or an equivalent volume of vehicle control (sterile H2O). After 1 hour, Brefeldin A (Golgi Plug, BD Biosciences) was added to the cell culture. PBMC were cultured for a total of 16 hours before being washed with PBS. Cells were stained with live/dead (Invitrogen) for 3 minutes at room temperature and then incubated with the surface antibody cocktail for 30min at 4C. The surface antibody cocktail included: CD20 BV510, 2H7; CD3 BUV395, SK7; CD27 BUV737, L128; CXCR5 BB515, RF8B2 (all from BD Biosciences); CD4 BV605, RPA-T4; CD8 BV650, RPA-T8; and CD45RA PerCP-Cy5.5, HI100 (all from BioLegend). After fixation and permeabilization (BD CytoFix/CytoPerm) for 20 minutes at 4C, cells were incubated with the intracellular antibody cocktail (IFNg APC, B27; TNF BV421, Mab11; IL-2 PE, MQ1-17H12; all from BioLegend). Cells were washed in Perm/Wash buffer, resuspended in PBS+1%FCS, and acquired on a BD LSR Fortessa.

Lee W.S., Audsley J., Trieu M.C., Reynaldi A., Aurelia L.C., Mehta P.H., Patterson J., Kent H.E., Nguyen J., Amarasena T., Esterbauer R., Haycroft E.R., Ramanathan P., Davenport M.P., Schlub T.E., Sasadeusz J., Wheatley A.K., Chung A.W., Juno J.A., Selva K.J, & Kent S.J. (2024). Randomised controlled trial reveals no benefit to a 3-month delay in COVID-19 mRNA booster vaccine. The Journal of clinical investigation.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!