Il 1β enzyme linked immunosorbent assay kits

T10 and human wild-type αSyn were purchased from Sigma-Aldrich (St. Louis, MO, USA). 4-Hydroxynonenal (HNE) was purchased from Abcam (USA), while miR155-5p mimics, inhibitor, and negative control were purchased from Rio Bio (Guangzhou, China). The pRL-TK plasmids were purchased from GenePharma (Shanghai, China). SHIP1 siRNA and scrambled control siRNA were purchased from GeneChem (Shanghai, China). Lipofectamine 2000 and Lipofectamine RNAiMAX were purchased from Invitrogen (Carlsbad, CA, USA). TNFα and IL-1β enzyme-linked immunosorbent assay kits were purchased from Abcam (USA). Cu-CPT22, resatorvid, E6446, and 3α-aminocholestane (3AC) were purchased from MedchemExpress (New Jersey, USA). Antibodies against IRAK1, TRAF6, p-IKKα/β, IKKα/β, NF-κB (p65), p-NF-κB (phospho-p65), PI3K (p85), p-PI3K (phosphor-p85), t-Akt, p-Akt, SHIP1, and β-actin were purchased from Cell Signaling Technology (Beverly, MA, USA). Goat anti-rabbit and goat anti-mouse secondary antibodies were purchased from Boster (Wuhan, China). Alexa Fluor 568 anti-rabbit secondary antibody and DAPI were purchased from Invitrogen (Carlsbad, CA, USA). Luciferase Reporter Gene Assay kit was purchased from Roche (Basel, Switzerland).