E bc k261

After sevoflurane anesthesia, blood was collected from the heart, and fasting blood glucose level was measured. The supernatant was collected, and the serum insulin content was detected using a Mouse INS (Insulin) ELISA Kit (E-EL-M1382c, Elabscience, Wuhan, China). The insulin resistance (IR) and sensitivity indices were calculated using the following formulae: IR index = [fasting blood glucose (mmol/L) × serum insulin (mIU/L)]/22.5 and insulin sensitivity index = 1/[fasting blood glucose (mmol/L) × serum insulin (mIU/L)] (Fang et al., 2010 (link)). The supernatant of liver tissue homogenate and serum were used to measure TG content using a TG assay Kit (E-BC-K261, Elabscience).

Blood was collected from the heart, and the fasting blood glucose level was measured. The blood samples were left at room temperature for 2 h, and then centrifuged at 1,000 × g for 20 min. The supernatant was collected, and the serum insulin content was detected using an INS ELISA kit (E-EL-M1382c; Elabscience, Wuhan, China). Finally, the homeostatic model assessment for insulin resistance (HOMA-IR) index and the homeostatic model assessment for insulin sensitivity index (HOMA-ISI) of each group were calculated using the following formulae (Fang et al., 2010 (link)): insulin resistance index = [fasting blood glucose (mmol/L) × serum insulin (mIU/L)] / 22.5; insulin sensitivity index = 1 / [fasting blood glucose (mmol/L) × serum insulin (mIU/L)]. Triglycerides (TGs) in the liver tissue or serum were measured using a TG assay kit (E-BC-K261; Elabscience, Wuhan, China).