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The UAS-TK RNAi is a genetic tool used for targeted gene knockdown in Drosophila melanogaster. It contains a UAS promoter that drives the expression of a double-stranded RNA (dsRNA) targeting the Herpes Simplex Virus Thymidine Kinase (TK) gene. This tool can be used to induce RNA interference (RNAi) and suppress the expression of the TK gene in Drosophila.

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Lab products found in correlation

Tub gal4, by Bloomington Drosophila Stock Center (1 mentions) Yolk gal4, by Bloomington Drosophila Stock Center (1 mentions) Uas toll10b, by Bloomington Drosophila Stock Center (1 mentions) Fb gal4, by Bloomington Drosophila Stock Center (1 mentions) Nsyb gal4, by Bloomington Drosophila Stock Center (1 mentions) Lexaop mcd8 gfp, by Bloomington Drosophila Stock Center (1 mentions) Uas mcherry nls, by Bloomington Drosophila Stock Center (1 mentions) Uas mcd8 chrfp, by Bloomington Drosophila Stock Center (1 mentions) Uas rpr, by Bloomington Drosophila Stock Center (1 mentions) Rn gal4, by Bloomington Drosophila Stock Center (1 mentions) Quas mcd8 gfp, by Bloomington Drosophila Stock Center (1 mentions) Uas gfp, by Bloomington Drosophila Stock Center (1 mentions)

2 protocols using uas tk rnai

1

Drosophila Lipid Metabolism Regulation

Cited 2 times
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The following fly lines were used: w1118 (background control), yw (background control), FB-Gal4 (Schmid et al., 2014 (link)), tub-Gal4 (Bloomington Stock Center no. 5138), yolk-Gal4 (Bloomington Stock Center no. 58814), UAS-LipinRNAi (VDRC transformant ID 36007), UAS-rel (Bloomington Stock Center no.9459), UAS-Toll10b (Bloomington Stock Center no. 58987), plin138 (Bi et al., 2012 (link)), UAS-plin1 (Bi et al., 2012 (link)), UAS-TK RNAi (Bloomington Stock Center no. 25800), UAS-wRNAi (Bloomington Stock Center no. 28980), TKg-Gal4 (Song et al., 2014 (link)), PGRP-LE112 (Bloomington Stock Center no. 33055), PGRP-LCΔE (Bloomington Stock Center no. 55713). Genetic recombination was used to generate UAS-plin1; tub-Gal4.
Harsh S., Heryanto C, & Eleftherianos I. (2019). Intestinal lipid droplets as novel mediators of host–pathogen interaction in Drosophila. Biology Open, 8(7), bio039040.
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2

Genetic Tools for Drosophila Research

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QUAS-DtAts,12 (link)WP-QF2,12 (link)C10-Gal4 (gifted by E. Hafen), UAS-lacZ-RNAi,55 (link) and UAS-lacZ (gifted by C. Goodman) have been characterized in previous studies. TkR86C-T2A-Gal4 was gifted by S. Kondo.23 (link)
TkR86C-T2A-lexA was a gift from Rao.24 (link)
UAS-rpr (#5824), UAS-GFP (#1521), rn-Gal4 (#51280), nSyb-Gal4 (#51635), QUAS-mCD8-GFP (#30002, #30003), UAS-nls-mCherry (#38425), UAS-mCD8-ChRFP (#27391), UAS-mCD8-ChRFP (#27392), LexAop-nls-GFP (#29954), LexAop-mCD8-GFP (#32203), UAS-Tk-RNAi (#25800), and Tk-T2A-Gal4 (#84693) were obtained from the Bloomington Drosophila Stock Center. UAS-TkR86C-RNAi 1 (v107090), UAS-TkR86C-RNAi 2 (v13392), and RNAi lines used for GPCR screening (Table S1) were obtained from the Vienna Drosophila Resource Center.
Kashio S., Masuda S, & Miura M. (2023). Involvement of neuronal tachykinin-like receptor at 86C in Drosophila disc repair via regulation of kynurenine metabolism. iScience, 26(9), 107553.
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