Human neuroblastoma cells (SH-SY5Y; CRL-2266; American Type culture Collection: ATCC), and Ixodes scapularis tick embryonic (ISE6; NR12234; BEI Resources) cell lines were purchased from ATCC/BEI and propagated as per the instructions from the supplier. Human SH-SY5Y cells were grown in complete EMEM medium (ATCC recommended) with 10% heat-inactivated Fetal bovine serum (FBS) (SIGMA) at 37 °C and 5% CO2, respectively. ISE6 cells were cultured and grown in L15B300 medium (SIGMA) at 34 °C and as per the recommendations from Dr. Munderloh[50 (link),51 (link)]. Human skin keratinocytes (HaCaT cells) were obtained from Addexbio Technologies/FisherScientific and grown (as per the instructions from the supplier) in complete Dulbecco’s Modified Eagle Medium (DMEM; SIGMA) with 10% heat-inactivated FBS at 37 °C and 5% CO2. SH-SY5Y or ISE6 cells were plated at densities of 1–2 × 10e6, and after overnight incubations, cells were infected with Langat virus [LGTV, strain LGT-TP21, 1 MOI (multiplication of infection) for ISE6 cells or 5 MOI for SH-SY5Y cells]. Naïve SH-SY5Y or HaCaT cells were infected via infectious exosomes collected from either SH-SY5Y or tick cells, respectively.
L15b300 medium
Manufactured by Merck Group
L15B300 medium is a cell culture medium designed for the growth and maintenance of a variety of cell types. It provides the necessary nutrients and components to support cell proliferation and survival in vitro.
Automatically generated - may contain errors
Lab products found in correlation
Sh sy5y, by American Type Culture Collection (2 mentions)
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (2 mentions)
Crl 2266, by American Type Culture Collection (2 mentions)
Complete emem medium, by American Type Culture Collection (2 mentions)
2 protocols using l15b300 medium
1
Propagation and Infection of Cell Lines
2
Propagation and Infection of Cell Lines
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Human neuroblastoma cells (SH-SY5Y; CRL-2266; American Type culture Collection: ATCC), and Ixodes scapularis tick embryonic (ISE6; NR12234; BEI Resources) cell lines were purchased from ATCC/BEI and propagated as per the instructions from the supplier. Human SH-SY5Y cells were grown in complete EMEM medium (ATCC recommended) with 10% heat-inactivated Fetal bovine serum (FBS) (SIGMA) at 37 °C and 5% CO2, respectively. ISE6 cells were cultured and grown in L15B300 medium (SIGMA) at 34 °C and as per the recommendations from Dr. Munderloh[50 (link),51 (link)]. Human skin keratinocytes (HaCaT cells) were obtained from Addexbio Technologies/FisherScientific and grown (as per the instructions from the supplier) in complete Dulbecco’s Modified Eagle Medium (DMEM; SIGMA) with 10% heat-inactivated FBS at 37 °C and 5% CO2. SH-SY5Y or ISE6 cells were plated at densities of 1–2 × 10e6, and after overnight incubations, cells were infected with Langat virus [LGTV, strain LGT-TP21, 1 MOI (multiplication of infection) for ISE6 cells or 5 MOI for SH-SY5Y cells]. Naïve SH-SY5Y or HaCaT cells were infected via infectious exosomes collected from either SH-SY5Y or tick cells, respectively.
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