Heptanophenone

Manufactured by Merck Group

Sourced in Belgium

Heptanophenone is a chemical compound used in laboratory settings. It is a colorless liquid with a distinct odor. Heptanophenone is commonly utilized as a solvent and a chemical intermediate in various applications.

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Lab products found in correlation

4 protocols using heptanophenone

DNPH-based Carbonyl Compound Analysis

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2,4-Dinitrophenylhydrazine (DNPH) was purchased from Tokyo Chemical Industry (TCI, Portland, OR); d₃-labeled DNPH (>98% isotopic purity) and malondialdehyde (MDA) was obtained from Santa Cruz Biotechnology (Dallas, TX); Ketones Mix (Propanone, 2-Butanone, 4-Methyl-2-Pentanone, 2-Hexanone, Cyclohexanone) and TO11/IP-6A Aldehyde/Ketone-DNPH Mix (Acetaldehyde-DNPH, Propanone-DNPH, Propanal-DNPH, Acrolein-DNPH, Benzaldehyde-DNPH, Butanal-DNPH, Crotonaldehyde-DNPH, 2,5-Dimethylbenzaldehyde-DNPH, Formaldehyde-DNPH, Hexanal-DNPH, Pentanal-DNPH, Isopentanal-DNPH, o-Tolualdehyde-DNPH, m-Tolualdehyde-DNPH, p-Tolualdehyde-DNPH), 4-hydroxy-3-methoxybenzaldehyde (Vanillin), Indole-3-carboxaldehyde, Hexanophenone, Heptanophenone, 2,3-Butanedione, 2,3-Pentanedione, trans-2-methyl-2-butenal, 3-methyl-2-butenal (methylcrotonaldehyde), sodium acetate, and concentrated HCl were obtained from Sigma Aldrich (Milwaukee, WI); ¹³C₂-Acetaldehyde was obtained from Cambridge Isotope Laboratories (Tewksbury, MA); Methylglyoxal (MG) was obtained from Thermo Fisher (Morris Plains, NJ); Strata-X polymeric reverse-phase cartridge was purchased from Phenomenex (Torrance, CA). All acids and organic solvents were HPLC grade or better.

Dator R., Carra’ A., Maertens L., Guidolin V., Villalta P.W, & Balbo S. (2016). A High Resolution/Accurate Mass (HRAM) Data-Dependent MS3 Neutral Loss Screening, Classification, and Relative Quantitation Methodology for Carbonyl Compounds in Saliva. Journal of the American Society for Mass Spectrometry, 28(4), 608-618.

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HPLC Characterization of Organic Compounds

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Uracil, acetanilide, methyl and ethyl esters of p-hydroxybenzoic acid (parabens), acetophenone, propiophenone, butyrophenone, benzophenone, valerophenone, hexanophenone, heptanophenone, and octanophenone were from Sigma-Aldrich (St. Louis, MO). Stock solutions, 25 mM, were made in acetonitrile (Chromsolv, LC-MS grad, Fisher Scientific, Fair Lawn NJ). Samples were diluted to concentrations from 5 to 20 µM with deionized water. Sample concentrations were selected to maximize concentration while factoring in column loadability and solubility. Water was from a Millipore Milli-Q Synthesis A10 purification system (Billerica, MA). Peptide samples were from an LC-MS grade BSA tryptic digest from Fisher. The lyophilized sample was diluted, as per reagent instructions, to 1 pmol/µL in 95:5 0.1% Formic acid/acetonitrile. Formic acid was from Sigma. The BSA sample was stored at −5 °C when not in use and used within 48 h of thawing/dilution.

Wilson R.E., Groskreutz S.R, & Weber S.G. (2016). Improving the Sensitivity, Resolution, and Peak Capacity of Gradient Elution in Capillary Liquid Chromatography with Large-Volume Injections by Using Temperature-Assisted On-Column Solute Focusing. Analytical chemistry, 88(10), 5112-5121.

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HPLC Characterization of Aromatic Compounds

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Uracil (99%), phenol (99%), acetophenone (99%), butyrophenone (99%), valerophenone (99%), hexanophenone (99%), heptanophenone (98%), octanophenone (99%), and benzanthracene (99%) were purchased from Sigma-Aldrich (Bornem, Belgium).
Acetonitrile (ACN, HPLC grade) was obtained from Biosolve (Valkenswaard, The Netherlands). Ultra-pure water (18.2 MΩ•cm) was generated by a Milli-Q water purification system (Millipore, Molsheim, France). Uracil (t0 marker), acetophenone, butyrophenone, valerophenone, hexanophenone, heptanophenone, octanophenone, and benzanthracene were dissolved separately in 70:30% (v/v) ACN:H2O at 1 g/L as stock solutions. The sample was prepared by mixing and diluting aforementioned solutions in 70:30% (v/v) ACN:H2O yielding 5 μL/L for acetophenone, 10 mg/L for Uracil, 10 μL/L for phenol and butyrophenone, 20 μL/L for valerophenone, hexanophenone, heptanophenone, 20 mg/L for benzanthracene, and 30 μL/L for octanophenone.

Zhou Z., De Pra M., Steiner F., Desmet G, & Eeltink S. (2020). Assessing effects of ultra-high-pressure liquid chromatography instrument configuration on dispersion, system pressure, and retention. Journal of chromatography. A, 1634.

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Synthesis and Characterization of Silica-Based Stationary Phases

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Toluene (HPLC grade, ≥99.8%), urea, tetramethoxysilane (TMOS), metyltrimethoxysilane (MTMS), 1 M aqueous acetic acid solution, polyethylene glycol (PEG) of molecular weight (MW) = 10,000 g/mol, thiourea, acetophenone, propiophenone, butyrophenone, valerophenone, hexanophenone, heptanophenone and octanophenone were purchased from Sigma-Aldrich (Overijse, Belgium). Trifluoroacetic acid (TFA, LC-MS Ultra grade) was purchased from Fluka (Buchs, Switzerland). Octadecyldimethyl-N,Ndimethylaminosilane (ODS-DMA) was purchased from ChemPur Feinchemikalien und Forschungsbedarf GmbH (Karlsruhe, Germany). Acetonitrile (ACN, HPLC supra-gradient grade) was purchased from Biosolve B.V. (Valkenswaard, the Netherlands). N-(Trimethylsilyl)-imidazole (TMSI) was obtained from Merck Schuchardt OHG (Hohenbrunn, Germany). Deionized water was produced in-house with a Milli-Q water purification system of Merck Millipore (Billerica, MA, USA). PTFE filters (0.20 µm × 25 mm) were purchased from Macherey-Nagel (Düren, Germany). MassPREP Peptide Mixture was purchased from Waters (Milford, MA, USA). Fused-silica capillaries of 20 µm i.d. (90 µm outer diameter (o.d.)) and 40 µm i.d. (105 µm o.d.) were purchased from Polymicro Technologies (Phoenix, AZ, USA).

Futagami S., Hara T., Ottevaere H., Terryn H., Baron G.V., Desmet G, & De Malsche W. (2019). Chromatographic study of the structural properties of mesoporous silica layers deposited on radially elongated pillars. Journal of chromatography. A, 1595.

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