Anti c ebpα rabbit polyclonal antibody

PPARγ, C/EBPα, and β-actin were analyzed by Western blot by the same procedure as above on cells in mid-stage differentiation. The antibodies used were anti-C/EBPα rabbit polyclonal antibody (Cell Signaling Technology), anti-PPARγ monoclonal antibody (Cell Signaling Technology), and anti-β-actin monoclonal antibody (Millipore, Billerica, MA, USA).

Chloroquine, MG132, cycloheximide, rosiglitazone, GW 9662, isobutylmethylxanthine (IBMX), dexamethasone, insulin and oil red O were purchased from Sigma-Aldrich (St Louis, MO). The anti-NEDD4 rabbit polyclonal antibody (catalog# 07-049) was purchased from Millipore (Billerica, MA). The anti-C/EBPα rabbit polyclonal antibody (catalog# 2295) was obtained from Cell Signaling Technology (Danvers, MA), and the anti-PPARγ (E-8) mouse monoclonal antibody and the anti-PPARγ (H-100) rabbit polyclonal antibody were purchased from Santa Cruz Biotechnology (Dallas, TX). The anti-PPARγ rabbit monoclonal antibody (catalog# MA5-15003) was from Thermo Fisher Scientific (Waltham, MA). The anti-HA.11 epitope tag mouse monoclonal antibody (previously Covance catalog# MMS-101P) was purchased from BioLegend (San Diego, CA). The anti-FLAG epitope tag (M2, catalog# F1804), the anti-GAPDH (catalog# G8795) and the anti-β-actin (catalog# A2228) mouse monoclonal antibodies were from Sigma-Aldrich (St Louis, MO). The mono- and polyubiquitinylated conjugates monoclonal antibody (FK2) was purchased from Enzo Life Sciences (Farmingdale, NY). The recombinant human PPARγ2 with His and GST tag (catalog# 501331) was purchased from NovoPro (Shanghai, China). The anti-Ub antibody was purchased from BD Biosciences (San Jose, CA).