The total protein in transfected KYSE450 and KYSE510 cells was extracted and quantified with the RIPA buffer (Solarbio, China), which was supplemented with 1% proteinase inhibitor cocktail. The protein concentration was obtained with the Pierce BCA protein assay kit (Thermo Fisher Scientific, USA). Next, the quantified protein was loaded into 10% or 12% SDS-PAGE for electrophoresis separation. The separated protein bands were then electronically transferred to the membranes (Sigma-Aldrich, USA). Following that, the membranes were put under ice for 2 h with 5% BSA at room temperature. This step was followed by the incubation of the membranes overnight with primary antibodies at 4 °C and 1.5 h incubation with secondary detection antibody (Cat# ab205718, Abcam, USA) at room temperature. Finally, the protein immunoblots were visualized using the Enhanced Chemiluminescent (ECL) Reagent Kit (Thermo Fisher Scientific. USA). The density of the blots was obtained using ImageJ software. All the primary antibodies were purchased from Abcam (USA), including anti-CyclinB1 (Cat# ab32053), anti-ICAM1 (Cat# ab53013), anti-VCAM1 (Cat# ab134047), anti-Cleaved PARP (Cat# ab32561), anti-Bax (Cat# ab32503), anti-Cleaved Caspase-3 (Cat# ab2302), anti-TXNRD1 (Cat# ab124954) and anti-β-actin (Cat# ab8227).
Anti txnrd1
Manufactured by Abcam
Sourced in United States
Anti-TXNRD1 is a primary antibody that detects the expression of the Thioredoxin Reductase 1 (TXNRD1) protein. TXNRD1 is an enzyme responsible for maintaining the balance of cellular redox states. This antibody can be used in various laboratory applications, such as Western blotting, immunohistochemistry, and immunocytochemistry, to analyze the expression and localization of TXNRD1 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Secondary detection antibody, by Abcam (2 mentions)
Anti icam 1, by Abcam (2 mentions)
Anti cyclin b1, by Abcam (2 mentions)
Anti vcam 1, by Abcam (2 mentions)
Anti cleaved parp, by Abcam (2 mentions)
Anti cleaved caspase 3, by Abcam (2 mentions)
Anti β actin, by Abcam (2 mentions)
Ripa buffer, by Solarbio (2 mentions)
Anti bax, by Abcam (2 mentions)
Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Enhanced chemiluminescent ecl reagent kit, by Thermo Fisher Scientific (1 mentions)
Anti pycr1, by Abcam (1 mentions)
Anti rabbit igg horseradish peroxidase linked whole antibody, by GE Healthcare (1 mentions)
Cell culture media, by Lonza (1 mentions)
Anti gapdh, by Cell Signaling Technology (1 mentions)
Amphotericin b, by Lonza (1 mentions)
Penicillin, by Lonza (1 mentions)
Streptomycin, by Lonza (1 mentions)
Taurine, by Merck Group (1 mentions)
Nrf2 small interfering rna sirna, by GenePharma (1 mentions)
4 protocols using anti txnrd1
1
Protein Expression Analysis in Esophageal Cancer Cells
2
Cell Culture Reagents and Antibodies
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(+)-Apl-1 was provided by Instituto Biomar (León, Spain) and was dissolved in DMSO and stored at −20 °C. Supplements and other chemicals not listed in this section were obtained from Sigma Chemicals Co. (St. Louis, MO, USA). Cell culture media, penicillin, streptomycin and amphotericin B were purchased from Biowhittaker (Walkersville, MD, USA). Fetal bovine serum (FBS) and human serum were products of Harlan-Seralb (Belton, UK). Antibodies (anti-TXNRD1, anti-TXNDC5, anti-PYCR1, and anti-PRX IV) were acquired from AbCam (Cambridge, UK). Antibodies (anti-GAPDH, anti-p-Iκκα/β, anti-Iκκβ) were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies (anti-Nrf2) were purchased from Santa Cruz Biotechnologies (Dallas, TX, USA). Secondary antibodies (anti-rabbit IgG, horseradish peroxidase-linked whole antibody, and anti-mouse IgG, horseradish peroxidase-linked whole antibody) were acquired from GE Healthcare (Buckinghamshire, UK). Plastics for cell culture were supplied by NUNC (Roskilde, Denmark) and VWR (West Chester, PA, USA).
3
Cellular Stress Response Regulation
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Dulbecco's modified Eagle's Ham/F12 medium (DMEM/F12), trypsin/EDTA, fetal bovine serum (FBS), trypsin/EDTA, antibiotics solution, and sterile phosphate buffer saline were purchased from Invitrogen (Carlsbad, CA). Cell culture dishes were obtained from Corning Inc. (Corning, NY). Taurine, epidermal growth factor (EGF), insulin, hydrocortisone, and other chemicals were supplied by Sigma‐Aldrich (St. Louis, MO). Nrf2 small‐interfering RNA (siRNA) and a negative control (NC) siRNA were designed and synthesized by Genepharma (Shanghai, China). p38 MAPK inhibitor (U0126), JNK inhibitor (Sp600125), and ERK1/2 inhibitor (SB203580) were obtained from Sigma‐Aldrich. The following antibodies were employed in this study: anti‐GRP78, anti‐CHOP, anti‐Nrf2, anti‐HO‐1, anti‐NQO‐1, anti‐Xct, and anti‐Txnrd1 antibodies were purchased from Abcam (Cambridge, UK); anti‐JNK, anti‐P‐JNK anti‐ERK, anti‐P‐ERK, anti‐p38, and anti‐P‐p38 antibodies were purchased from Cell Signaling Technology (Beverly, MA); and anti‐β‐actin antibody was obtained from Amyjet Scientific (Wuhan, China).
4
Protein Analysis of Transfected Esophageal Cancer Cells
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The total protein in transfected KYSE450 and KYSE510 cells was extracted and quanti ed with the RIPA buffer (Solarbio, China), which was supplemented with 1% proteinase inhibitor cocktail. The protein concentration was obtained with the Pierce BCA protein assay kit (Thermo Fisher Scienti c, USA). Next, the quanti ed protein was loaded into 10% or 12% SDS-PAGE for electrophoresis separation. The separated protein bands were then electronically transferred to the membranes (Sigma-Aldrich, USA). Following that, the membranes were put under ice for 2 h with 5% BSA at room temperature. This step was followed by the incubation of the membranes overnight with primary antibodies at 4°Cand 1.5-hour incubation with secondary detection antibody (Cat# ab205718, Abcam, USA) at room temperature. Finally, the protein immunoblots were visualized using the Enhanced Chemiluminescent (ECL) Reagent Kit (Thermo Fisher Scienti c. USA). The density of the blots was obtained using ImageJ software. All the primary antibodies were purchased from Abcam (USA), including anti-CyclinB1 (Cat# ab32053), anti-ICAM1 (Cat# ab53013), anti-VCAM1 (Cat# ab134047), anti-Cleaved PARP (Cat# ab32561), anti-Bax (Cat# ab32503), anti-Cleaved Caspase-3 (Cat# ab2302), anti-TXNRD1 (Cat# ab124954) and anti-β-actin (Cat# ab8227).
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