Mouse il 1β

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Mouse IL-1β is a laboratory reagent used to detect and quantify the mouse interleukin-1 beta protein in various sample types. It is designed for research use only and not for diagnostic or therapeutic purposes.

Automatically generated - may contain errors

Lab products found in correlation

Human il 1β, by Thermo Fisher Scientific (8 mentions) Human tnf α, by Thermo Fisher Scientific (7 mentions) Mouse tnf α, by Thermo Fisher Scientific (7 mentions) Mouse il 6, by Thermo Fisher Scientific (7 mentions) Tnf α, by Thermo Fisher Scientific (4 mentions) Human ifn β, by Thermo Fisher Scientific (2 mentions) Mouse ifn β, by R&D Systems (2 mentions) L kynurenin, by Merck Group (2 mentions) Ifn γ, by R&D Systems (2 mentions) Z vad fmk, by InvivoGen (2 mentions) Human or mouse il 1β elisa kits, by R&D Systems (2 mentions) Antibodies to mouse il 1β and mouse caspase 1, by R&D Systems (2 mentions) Human ifnα, by Thermo Fisher Scientific (2 mentions) Poly 1 c hmw, by InvivoGen (2 mentions) Rb anti myc, by Cell Signaling Technology (2 mentions) Ms anti myc, by Cell Signaling Technology (2 mentions) Rb anti actin, by Merck Group (2 mentions) Ms anti flag, by Merck Group (2 mentions) Rb anti 14 3 3, by Santa Cruz Biotechnology (2 mentions) Ms anti spir 1, by Santa Cruz Biotechnology (2 mentions)

42 protocols using mouse il 1β

Exploring Podocyte Lipidosis and Apoptosis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The mouse podocyte cell line MPC5 was kindly provided by Professor Ruan (Center for Nephrology, Royal Free and University College Medical School, London,
United Kingdom). Cells were cultured on rat tail type I collagen (Millipore, USA) in RPMI-1640 (Gibco, USA) supplemented with 10% fetal bovine serum (PAN, Germany), 100 U/l penicillin (Gibco, USA), 0.1 mg/ml streptomycin (Sigma, USA), and 100 IU/ml interferon-γ (R&D, USA) at 33°C in 5% CO₂ for 14 days. Thereafter, cells were cultured in the same conditions, except that the medium lacked interferon-γ. Cells were treated with human LDL (200 μg/ml, extracted from blood) with or without mouse IL-1β (20 ng/ml; PeproTech, USA) and a monoclonal anti-CD36 antibody (2 μg/ml; ab23680, Abcam, UK). Levels of CD36 and NLRP3 expression, lipidosis, and apoptosis were measured.

Yang X., Wu Y., Li Q., Zhang G., Wang M., Yang H, & Li Q. (2018). CD36 Promotes Podocyte Apoptosis by Activating the Pyrin Domain-Containing-3 (NLRP3) Inflammasome in Primary Nephrotic Syndrome. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 24, 6832-6839.

+ Open protocol

+ Expand

Cytokine and AhR Ligand Stimulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

If not stated otherwise, the following concentrations were used for stimulation experiments: mouse recombinant mouse TNF-α (Peprotech, #AF-315-01A) 50 ng/ml, mouse IL-1β (Peprotech, #211-11B) 100 ng/ml, mouse IFN-β (R&D, #8234-MB-010/CF) 100 U/ml, IFN-γ (R&D, #485-MI-100/CF) 20 ng/ml, Trichostatin-A (Sigma, #T8552), I3S (Sigma, #I3875-250MG) 50 µg/ml, FICZ (Sigma, #SML1489-1MG) 1 µM, L-Kynurenin (Sigma, #K8625-25MG) 1 µM, TCDD (Sigma, #48599), ITE (Sigma, #SML3139), human TNF-α (Peprotech, #300-01 A) 50 ng/ml, human IL-1β (Peprotech, #200-01B) 100 ng/ml, human IFN-β (Peprotech, #300-02BC) 100 U/ml.

Linnerbauer M., Beyer T., Nirschl L., Farrenkopf D., Lößlein L., Vandrey O., Peter A., Tsaktanis T., Kebir H., Laplaud D., Oellinger R., Engleitner T., Alvarez J.I., Rad R., Korn T., Hemmer B., Quintana F.J, & Rothhammer V. (2023). PD-L1 positive astrocytes attenuate inflammatory functions of PD-1 positive microglia in models of autoimmune neuroinflammation. Nature Communications, 14, 5555.

+ Open protocol

+ Expand

Inflammasome Activation in Obesity

Check if the same lab product or an alternative is used in the 5 most similar protocols

All chemicals used in this study were purchased from Sigma (St. Louis, MO), unless otherwise noted. Ultrapure LPS, ATP, caspase-1 inhibitor ZVAD-FMK were purchased from Invivogen (San Diego, CA). Mouse IL-1β from PeproTech (Rocky Hill, NJ); human or Mouse IL-1β ELISA kits were from R&D system (Minneapolis, MN) or eBioscience (San Diego, California); Antibodies to Mouse IL-1β and mouse caspase-1 were from R&D Systems or Santa Cruz Biotechnology (Santa Cruz, CA).
Wild type (WT) C57BL/6, caspase-1 KO and ob/ob (B6.Cg-Lep^ob/J) mice were purchased from the Jackson Laboratory (Bar Harbor, Maine). All mice were maintained at MUSC Hollings animal facility under specific pathogen-free conditions. All animal experiments were approved by the Institutional Animal Care & Use Committee (IACUC) at MUSC, and were conducted in accordance with federal regulations as well as institutional guidelines and regulations on animal studies.

Zhang J., Zhang K., Li Z, & Guo B. (2016). ER Stress-induced Inflammasome Activation Contributes to Hepatic Inflammation and Steatosis. Journal of clinical & cellular immunology, 7(5), 457.

+ Open protocol

+ Expand

Inflammatory Signaling Pathway Inhibition

Check if the same lab product or an alternative is used in the 5 most similar protocols

Recombinant human IL-1β (Cat#AF-200-01B) and mouse IL-1β (Cat#AF-211-11B) were acquired from PeproTech. P38 inhibitor SB203580 (Cat#s1076) and glycolysis inhibitor 2-deoxy-D-glucose (2DG; Cat#s4701) were obtained from Selleckchem(Houston,United States). The inhibitors were dissolved in DMSO. Working concentrations of SB203580 and 2DG were 30 µM and 5 mM, respectively.

Tan Q., Duan L., Huang Q., Chen W., Yang Z., Chen J, & Jin Y. (2021). Interleukin -1β Promotes Lung Adenocarcinoma Growth and Invasion Through Promoting Glycolysis via p38 Pathway. Journal of Inflammation Research, 14, 6491-6509.

+ Open protocol

+ Expand

Cytokine and Chemical Stimulation Experiments

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following concentrations were used for stimulation experiments: recombinant mouse TNF-α (Peprotech, no. AF-315-01A) 50 ng ml⁻¹, mouse IL-1β (Peprotech, no. 211-11B) 100 ng ml⁻¹, mouse HB-EGF (Novus Biologicals, no. 35069) 50 ng ml⁻¹, IFN-γ (R&D, no. 485-MI-100/CF) 20 ng ml⁻¹, I3S (Sigma, no. I3875-250MG) 50 µg ml⁻¹, CH-223191 (Sigma Aldrich, no. C8124) 50 µM, Cobalt(II)-chloride (CoCl₂; Sigma Aldrich, no. 232696) 500 µM, 5-Aza (Sigma Aldrich, no. A2385) 10 µM, LPS-EB (InvivoGen, no. tlrl-3pelps) 20 ng ml⁻¹, human TNF-α (Peprotech, no. 300-01A) 50 ng ml⁻¹, human IL-1β (Peprotech, no. 200-01B) 100 ng ml⁻¹, human HB-EGF (Peprotech, no. 100-47) 50 ng ml⁻¹.

Linnerbauer M., Lößlein L., Vandrey O., Peter A., Han Y., Tsaktanis T., Wogram E., Needhamsen M., Kular L., Nagel L., Zissler J., Andert M., Meszaros L., Hanspach J., Zuber F., Naumann U.J., Diebold M., Wheeler M.A., Beyer T., Nirschl L., Cirac A., Laun F.B., Günther C., Winkler J., Bäuerle T., Jagodic M., Hemmer B., Prinz M., Quintana F.J, & Rothhammer V. (2024). The astrocyte-produced growth factor HB-EGF limits autoimmune CNS pathology. Nature Immunology, 25(3), 432-447.

+ Open protocol

+ Expand

Stimulation Conditions for Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

If not stated otherwise, the following concentrations were used for stimulation experiments: mouse PTN (R&D, #6580-PL-050) 50 ng/ml, mouse TNF-α (Peprotech, #AF-315-01A) 50 ng/ml, mouse IL-1β (Peprotech, #211-11B), mouse IFN-β (R&D, #8234-MB-010/CF) 1.000 U/ml, mouse TGF-β (R&D, #7666-MB-005/CF) 10ng/ml, FTY720 (Sigma, #SML0700-5MG) 10 µM, DFO (Merck, #252750-1gm) 200µM, I3S (Sigma, #I3875-250MG) 50 µg/ml, FICZ (Sigma, #SML1489-1MG) 1µM, L-Kynurenin (Sigma, #K8625-25MG) 1µM, human TNF-α (Peprotech, #300-01A) 50 ng/ml, human IL-1β (Peprotech, #200-01B) 100 ng/ml, human IFN-β (Peprotech, #300-02BC) 1.000 U/ml.

Linnerbauer M., Lößlein L., Farrenkopf D., Vandrey O., Tsaktanis T., Naumann U, & Rothhammer V. (2022). Astrocyte-Derived Pleiotrophin Mitigates Late-Stage Autoimmune CNS Inflammation. Frontiers in Immunology, 12, 800128.

+ Open protocol

+ Expand

Immunoblotting Protocols Using Diverse Antibodies

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary antibodies used were from the following sources: rabbit (Rb) anti-Myc (Cell Signaling, 2278), mouse (Ms) anti-Myc (Cell Signaling, 9B11), Rb anti-actin (Sigma, A2066), Ms anti-FLAG (Sigma F1804), Rb anti-14-3-3 (Santa Cruz, sc-629), Ms anti -Spir-1 (Santa Cruz, sc-517039), Ms anti-Spir-1 (Abcam, ab57463), Rb anti-DDX3 (Cell Signaling, 2635), Rb anti-IKKβ (Cell Signaling, 2684), Rb anti-HA (Sigma, H6908), Ms anti-α-tubulin (Millipore, 05–829), Ms anti-GAPDH (Sigma, G8795), Rb anti-IRF3 (Cell Signaling, 4962), Rb anti-IRF3 (Santa Cruz, SC-9082), Ms anti-COPε (Santa Cruz, sc-133194), Rb anti-phospho-IRF3 Ser396 (Cell Signaling, 4947S) and Rb polyclonal anti-C6 [53 (link)]. For dilutions used for the primary antibodies, see S3 Table. Secondary antibodies used (1:10,000 dilution) were IRDye 680RD-conjugated goat anti-rabbit IgG or anti-mouse IgG and IRDye 800CW-conjugated goat anti-rabbit IgG or anti-mouse IgG (LI-COR).
Reagents used in this study were: anti-c-Myc agarose from Santa Cruz Biotechnology, and monoclonal anti-HA-agarose, clone HA-7, ANTI-FLAG M2 affinity gel and Poly-D-lysine hydrobromide (all from Sigma Aldrich). Human IFNα, human TNF-α and mouse IL-1β were from Peprotech, HMW poly(I:C) and puromycin were from InvivoGen, and doxycycline was from Melford.

Torres A.A., Macilwee S.L., Rashid A., Cox S.E., Albarnaz J.D., Bonjardim C.A, & Smith G.L. (2022). The actin nucleator Spir-1 is a virus restriction factor that promotes innate immune signalling. PLoS Pathogens, 18(2), e1010277.

+ Open protocol

+ Expand

Inflammasome Activation in Obesity

Check if the same lab product or an alternative is used in the 5 most similar protocols

Zhang J., Zhang K., Li Z, & Guo B. (2016). ER Stress-induced Inflammasome Activation Contributes to Hepatic Inflammation and Steatosis. Journal of clinical & cellular immunology, 7(5), 457.

+ Open protocol

+ Expand

Macrophage Activation and Cytokine Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

U937 or mouse macrophages derived from 7–9-wk-old age-matched animals were incubated for up to 24 h in 96-well flat-bottom plates at a density of 10⁵ cells per well in RPMI-1640 containing 1 or 100 ng/ml LPS (InvivoGen), 500 µM succinate (sodium succinate dibasic hexahydrate; Sigma-Aldrich), 10 ng/ml mouse IL-1β (PeproTech), 180 µg/ml MSU (Enzo Life Sciences), 5 µM GPR91 antagonist GPR91A1 (synthesized in house from Bhuniya et al., 2011 (link)), and 10% human RA SFs (Asterand). After 24 h (or 7 h in the case of culture with 1 ng/ml LPS [2 h] followed by 180 µg/ml MSU [5 h]), cells were collected and processed for quantitative PCR as described in the next section. Cytokines in supernatants were analyzed by ELISA using the mouse IL-1β ELISA Set or human IL-1β ELISA Set II (both from BD) according to the manufacturer’s instructions.

Littlewood-Evans A., Sarret S., Apfel V., Loesle P., Dawson J., Zhang J., Muller A., Tigani B., Kneuer R., Patel S., Valeaux S., Gommermann N., Rubic-Schneider T., Junt T, & Carballido J.M. (2016). GPR91 senses extracellular succinate released from inflammatory macrophages and exacerbates rheumatoid arthritis. The Journal of Experimental Medicine, 213(9), 1655-1662.

+ Open protocol

+ Expand

Cytokine Release Assay for PLGA@M

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human and mouse recombinant IL-6 (1600 pg/mL for human IL-6, 1300 pg/mL for mouse IL-6, PeproTech, USA) and IL-1β (1700 pg/mL for human IL-1β, 4500 pg/mL for mouse IL-1β, PeproTech, USA) were mixed with PLGA@M derived from THP-1 or RAW 264.7 cells at different concentrations (0, 1, 2, 4 mg/mL). The mixtures were then incubated at 37 °C for 2 h. After the incubation, the mixtures were centrifuged at 21,000g for 15 min to remove the nanoparticles. Cytokine concentration in the supernatant was quantified with corresponding ELISA kits (DAKEWE, China). All experiments were carried out in triplicate.

Tan Q., He L., Meng X., Wang W., Pan H., Yin W., Zhu T., Huang X, & Shan H. (2021). Macrophage biomimetic nanocarriers for anti-inflammation and targeted antiviral treatment in COVID-19. Journal of Nanobiotechnology, 19, 173.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!