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Goat anti rabbit igg dylight 680 conjugate

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Goat anti-rabbit IgG DyLight 680 conjugate is a secondary antibody used in various immunoassay techniques. It binds to rabbit primary antibodies and is conjugated with DyLight 680 dye, enabling detection and visualization of target proteins or antigens.

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Lab products found in correlation

Goat anti mouse igg dylight 800 conjugate, by Thermo Fisher Scientific (6 mentions) Anti flag m2 monoclonal antibody, by Merck Group (4 mentions) Servagel tg prime 8 16 precast gels, by Serva Electrophoresis (3 mentions) N dodecyl maltoside, by Anatrace (2 mentions) Pentaethylene glycol monooctyl ether c8e5, by Anatrace (2 mentions) Para benzoylphenylalanine, by Bachem (2 mentions) Servagel tg prime 4 20 and 8 16 precast gels, by Serva Electrophoresis (2 mentions) Mini protean tgx, by Bio-Rad (2 mentions) Para benzophenylalanine, by Bachem (2 mentions) Odyssey imaging system, by LI COR (1 mentions) Anti ha monoclonal antibody, by Merck Group (1 mentions) Serva blue g, by Serva Electrophoresis (1 mentions) 3xflag peptide, by Merck Group (1 mentions) Streptavidin dylight 800, by Thermo Fisher Scientific (1 mentions) M2 anti flag agarose beads, by Merck Group (1 mentions)

Close spelling variants of this product

Anti-rabbit IgG–DyLight 680 (2 citations) DyLight 680 (42 citations) IgG Rabbit (4 citations)

6 protocols using goat anti rabbit igg dylight 680 conjugate

1

Western Blot Analysis of Bacterial Virulence Factors

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For analysis of whole cell lysates, samples were taken after subculturing for 4 h at 27°C (analysis of Inv expression) or 37°C (analysis of YadA expression). Pellets were resuspended in H2O and Laemmli buffer (Bio-Rad) to obtain 5×106 bacteria/ml. Samples were boiled for 10 min at 95°C before loading on the gel. After SDS-PAGE proteins were transferred onto nitrocellulose membranes. The membranes were blocked overnight with 5% milk powder in PBS at 4°C. Membranes were probed with antisera against Inv (rabbit anti-Inv, 1:2000), YadA (rabbit anti-YadA, 1:1000), OmpA (rabbit anti-OmpA, 1:1000), OmpF (rabbit anti-OmpF, 1:1000), BamB (rabbit anti-BamB, 1:1000) and RNA-Polymerase β subunit (mouse anti-RNA-Polymerase, Neoclone, 1:1000) and secondary antibodies goat anti-rabbit IgG DyLight 680 conjugate or goat anti-mouse IgG DyLight 800 conjugate (Thermo Fisher scientific). For detection of bands, the Odyssey imaging system (Li-Cor Biosciences) was used.
Weirich J., Bräutigam C., Mühlenkamp M., Franz-Wachtel M., Macek B., Meuskens I., Skurnik M., Leskinen K., Bohn E., Autenrieth I, & Schütz M. (2017). Identifying components required for OMP biogenesis as novel targets for antiinfective drugs. Virulence, 8(7), 1170-1188.
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2

Purification and Characterization of Type III Secretion Proteins

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Chemicals were from Sigma-Aldrich unless otherwise specified. Detergents n-dodecyl-maltoside (DDM), Lauryl Maltose Neopentyl Glycol (LMNG) and pentaethylene glycol monooctyl ether (C8E5) were from Anatrace. para-benzoylphenylalanine was from Bachem. SERVAGel™ TG PRiME™ 4-20% and 8-16 % precast gels were from Serva and 4–20% Mini-PROTEAN® TGX™ were from Bio-Rad. Primers are listed in Table S4 and were synthetized by Eurofins, Invitrogen or Sigma-Aldrich. Polyclonal rabbit anti-PrgK antibody was a kind gift of Thomas Marlovits, Vienna. Monoclonal M2 anti-FLAG antibody was from Sigma-Aldrich. Monoclonal anti-SipB and anti-InvJ antibodies were raised by Abmart. Secondary antibodies goat anti-mouse IgG DyLight 800 conjugate and goat anti-rabbit IgG DyLight 680 conjugate were from Thermo Fisher Scientific.
Kuhlen L., Abrusci P., Johnson S., Gault J., Deme J., Caesar J., Dietsche T., Mebrhatu M.T., Ganief T., Macek B., Wagner S., Robinson C.V, & Lea S.M. (2018). Structure of the Core of the Type Three Secretion System Export Apparatus. Nature structural & molecular biology, 25(7), 583-590.
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3

Purification and Characterization of Type III Secretion Proteins

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Chemicals were from Sigma-Aldrich unless otherwise specified. Detergents n-dodecyl-maltoside (DDM), Lauryl Maltose Neopentyl Glycol (LMNG) and pentaethylene glycol monooctyl ether (C8E5) were from Anatrace. para-benzoylphenylalanine was from Bachem. SERVAGel™ TG PRiME™ 4-20% and 8-16 % precast gels were from Serva and 4–20% Mini-PROTEAN® TGX™ were from Bio-Rad. Primers are listed in Table S4 and were synthetized by Eurofins, Invitrogen or Sigma-Aldrich. Polyclonal rabbit anti-PrgK antibody was a kind gift of Thomas Marlovits, Vienna. Monoclonal M2 anti-FLAG antibody was from Sigma-Aldrich. Monoclonal anti-SipB and anti-InvJ antibodies were raised by Abmart. Secondary antibodies goat anti-mouse IgG DyLight 800 conjugate and goat anti-rabbit IgG DyLight 680 conjugate were from Thermo Fisher Scientific.
Kuhlen L., Abrusci P., Johnson S., Gault J., Deme J., Caesar J., Dietsche T., Mebrhatu M.T., Ganief T., Macek B., Wagner S., Robinson C.V, & Lea S.M. (2018). Structure of the Core of the Type Three Secretion System Export Apparatus. Nature structural & molecular biology, 25(7), 583-590.
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4

Immunoblotting of HA-tagged proteins

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Chemicals were from Sigma-Aldrich unless otherwise specified. SERVAGel™ TG PRiME™ 8–16 % precast gels were from Serva. Primers were synthetized by Eurofins and Integrated DNA Technologies. Monoclonal anti-HA antibody was from Sigma-Aldrich. Secondary antibodies goat anti-mouse IgG DyLight 800 conjugate and goat anti-rabbit IgG DyLight 680 conjugate were from Thermo-Fisher (SA5-35571, 35568, respectively).
Malmsheimer S., Grin I., Bohn E., Franz-Wachtel M., Macek B., Sahr T., Smollich F., Chetrit D., Meir A., Roy C., Buchrieser C, & Wagner S. (2024). The T4bSS of Legionella features a two-step secretion pathway with an inner membrane intermediate for secretion of transmembrane effectors. bioRxiv.
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5

Protein Expression and Detection

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Chemicals were from Sigma-Aldrich unless otherwise specified, para-benzophenylalanine was from Bachem. The radioactive [S35]-methionine was obtained from Hartman Analytics. SERVAGel™ TG PRiME™ 8–16% precast gels were from Serva. Primers are listed in Supplementary Data 3 and were synthetized by Eurofins and Integrated DNA Technologies. Monoclonal M2 anti-FLAG antibody was from Sigma-Aldrich (F3165). Secondary antibodies goat anti-mouse IgG DyLight 800 conjugate and goat anti-rabbit IgG DyLight 680 conjugate were from Thermo-Fisher (SA5-35571, 35568, respectively).
Krampen L., Malmsheimer S., Grin I., Trunk T., Lührmann A., de Gier J.W, & Wagner S. (2018). Revealing the mechanisms of membrane protein export by virulence-associated bacterial secretion systems. Nature Communications, 9, 3467.
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6

Affinity Purification of Protein Complexes

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Chemicals were from Sigma-Aldrich unless otherwise specified. Detergent n-dodecyl-maltoside (DDM) was from Affimetrix-Anatrace. para-benzophenylalanine was from Bachem. SERVA Blue G and SERVAGel TG PRiME 8–16% precast gels were from Serva. NativePAGE Novex Bis-Tris 3–12% gels were from Life Technologies. Primers are listed in S5 Table and were synthetized by Eurofins and Integrated DNA Technologies. Polyclonal rabbit anti-MBP antibody were from New England Biolabs. Monoclonal mouse anti-RNApol antibody was from BioLegend. Monoclonal M2 anti-FLAG antibody, M2 anti-FLAG agarose beads, and 3xFLAG peptide were from Sigma-Aldrich. CaptureSelect-biotin, Streptavidin DyLight 800, and secondary antibodies goat anti-mouse IgG DyLight 800 conjugate and goat anti-rabbit IgG DyLight 680 conjugate were from Thermo-Fisher.
Dietsche T., Tesfazgi Mebrhatu M., Brunner M.J., Abrusci P., Yan J., Franz-Wachtel M., Schärfe C., Zilkenat S., Grin I., Galán J.E., Kohlbacher O., Lea S., Macek B., Marlovits T.C., Robinson C.V, & Wagner S. (2016). Structural and Functional Characterization of the Bacterial Type III Secretion Export Apparatus. PLoS Pathogens, 12(12), e1006071.
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