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2 protocols using ip3r1

1

Comprehensive Antibody Characterization Protocol

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Primary antibodies used were: α-Tubulin (4G1) (Santa Cruz Biotechnology, cat. no. sc-58666), BAP1 (C-4) (Santa Cruz Biotechnology, cat. no. sc-28383), BAP1 (D7W7O) (Cell Signaling, cat. no. 13271), BAP1 (H-300) (Santa Cruz Biotechnology, cat. no. sc-28236), anti-human CD90 (BD Biosciences, cat. no. 561558), Caspase-3 (Cell Signaling, cat. no. 9662), FLAG (Sigma-Aldrich, cat. no. F7425), Histone H2A.X (D17A3) (Cell Signaling, cat. no. 7631), Anti-phospho-Histone H2A.X (Ser139) (EMD Millipore, cat. no. 05-636), H3 (Cell Signaling, cat. no. 4499), IP3R1 (Novus Biologicals, cat. no. NB120-5908), IP3R3 (BD Biosciences, cat. no. 610312), Anti-Lamin B1 (abcam, cat. no. ab16048), c-myc (Bethyl Laboratories, cat. no A190-105A), Myc-Tag (9B11) (Cell Signaling, cat. no. 2276), PDI (abcam, cat. no. ab31811) Anti-VDAC1 (abcam, cat. no. ab15895). Secondary antibodies used were: Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP (Thermo Scientific, cat. no. 32430); Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP (Thermo Scientific, cat. no. 32460); Mouse TrueBlot® ULTRA: Anti-Mouse Ig HRP (Rockland, cat. no. 18-8817-31).
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2

Comprehensive Antibody Characterization Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary antibodies used were: α-Tubulin (4G1) (Santa Cruz Biotechnology, cat. no. sc-58666), BAP1 (C-4) (Santa Cruz Biotechnology, cat. no. sc-28383), BAP1 (D7W7O) (Cell Signaling, cat. no. 13271), BAP1 (H-300) (Santa Cruz Biotechnology, cat. no. sc-28236), anti-human CD90 (BD Biosciences, cat. no. 561558), Caspase-3 (Cell Signaling, cat. no. 9662), FLAG (Sigma-Aldrich, cat. no. F7425), Histone H2A.X (D17A3) (Cell Signaling, cat. no. 7631), Anti-phospho-Histone H2A.X (Ser139) (EMD Millipore, cat. no. 05-636), H3 (Cell Signaling, cat. no. 4499), IP3R1 (Novus Biologicals, cat. no. NB120-5908), IP3R3 (BD Biosciences, cat. no. 610312), Anti-Lamin B1 (abcam, cat. no. ab16048), c-myc (Bethyl Laboratories, cat. no A190-105A), Myc-Tag (9B11) (Cell Signaling, cat. no. 2276), PDI (abcam, cat. no. ab31811) Anti-VDAC1 (abcam, cat. no. ab15895). Secondary antibodies used were: Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP (Thermo Scientific, cat. no. 32430); Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP (Thermo Scientific, cat. no. 32460); Mouse TrueBlot® ULTRA: Anti-Mouse Ig HRP (Rockland, cat. no. 18-8817-31).
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