Male NOD.Cg-Rag1tm1Mom Il2rgtm1Wjl/SzJ (NRG, The Jackson Laboratory, Bar Harbor, ME) mice aged six to eight weeks were anesthetized with a solution of ketamine (9.5 mg/ml; 99/192/85-C, Bioveta, Ivanovice na Hané, Czech Republic) and xylazine (0.95 mg/ml; A6A066, Bioveta, Ivanovice na Hané, Czech Republic) at a final dose of 100 µl/10 g of body weight, administered intraperitoneally. The mice were injected intradermally with 5 × 104 A375 IV GFP Luc2 cells in 50 µl PBS. Live cell imaging was performed using the IVIS Lumina XR optical imaging system (Perkin Elmer, Waltham, MA) 15 min after intraperitoneal injection of D-luciferin, sodium salt (LUCNA-10G, Goldbio, St. Louis, MO, 150 mg/kg) with exposure times of 15 s, 30 s, and 60 s. Luminescence (total flux [p/s]) was quantified using Living Image v4.7.2 software (Perkin Elmer, Waltham, MA). All experiments were conducted with the approval of the Academy of Sciences of the Czech Republic (AVCR 103/2019), overseen by the ethical committee of the Institute of Biophysics CAS, performed by certified individuals (MP, RV), and carried out in accordance with relevant guidelines and regulations. All methods are reported in accordance with ARRIVE guidelines.
Ivis lumina xr optical imaging system
Manufactured by PerkinElmer
Sourced in United States
The IVIS Lumina XR optical imaging system is a non-invasive tool designed for in vivo bioluminescence and fluorescence imaging. It enables the detection and quantification of light-emitting reporter genes or fluorescently-labeled targets within small animal models.
Automatically generated - may contain errors
4 protocols using ivis lumina xr optical imaging system
1
Bioluminescent Imaging of Melanoma Xenografts
2
Topical Delivery of Cy5.5-Loaded Glycerosomes
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Cy5.5-NHS ester, a near-infrared fluorescence, was used to replace PF in each preparation. Male nude mice were anesthetized and fixed in a dark place. Then, 0.3 mL of Cy5.5-loaded preparations (10 μmol/L Cy5.5 in STO-glycerosomes and common glycerosomes) was dropped onto 1.5×1.5×0.3 cm cotton pads that were applied on the left knee of mice, and other steps were the same as mentioned earlier. After 5 h of administration, the cotton pads were discarded, and excess preparations were wiped from the surface of the skin. Mice were subsequently scanned in the IVIS Lumina XR optical imaging system (PerkinElmer, Waltham, MA, USA). The following parameters were used: excitation wavelength at 678 nm, emission wavelength at 695 nm, and exposure time at 60 ms. Lumina II Living Image 4.3 software was used to quantify the fluorescence remaining in each knee joint by calculating the flux radiating omnidirectionally from the region of interest (ROI) and graphed as radiant efficiency ([p/s/cm2/sr]/[μW/cm2]). To yield a standardized ROI for measurement of the knee fluorescence, the automatic square mode was used for integration.
3
Fluorescence Imaging of Organ Biodistribution
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Fluorescence of major organs brain, heart, kidney,
and organs of RES (liver, spleen, and lung) was measured at 120 min
after IV injection of fluorescence-labeled agents. The analysis was
performed using an IVIS Lumina XR Optical Imaging System (PerkinElmer,
Richmond, CA, USA) using DsRed filter sets.39 (link) Organ and brain fluorescence were simultaneously measured in all
tissue samples from each experiment to attain consistent imaging parameters.
Imaging and comparison of whole organs refer to authentic mice.
and organs of RES (liver, spleen, and lung) was measured at 120 min
after IV injection of fluorescence-labeled agents. The analysis was
performed using an IVIS Lumina XR Optical Imaging System (PerkinElmer,
Richmond, CA, USA) using DsRed filter sets.39 (link) Organ and brain fluorescence were simultaneously measured in all
tissue samples from each experiment to attain consistent imaging parameters.
Imaging and comparison of whole organs refer to authentic mice.
4
Biodistribution and Brain Imaging
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