Primary CD8 cells were isolated and activated as explained above. At 48 h post‐activation, cells were lysed with l‐PPase reaction buffer (New England BioLabs, Ipswich, MA) supplemented with 0.4% NP‐40 and EDTA‐free protease inhibitors (Sigma‐Aldrich). The phosphatase reaction was performed following the manufacturer's instructions.
L ppase reaction buffer
Manufactured by New England Biolabs
The L-PPase reaction buffer is a buffer solution designed to facilitate the enzymatic activity of L-phosphatase. It provides the necessary ionic environment and pH conditions for the optimal function of this enzyme. The buffer composition and properties are formulated to support the specific requirements of L-phosphatase-based reactions, ensuring consistent and reliable performance in relevant laboratory applications.
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Lab products found in correlation
2 protocols using l ppase reaction buffer
1
CD8+ T Cell Phosphatase Assay
2
Lysis and Phosphatase Assay
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BMDMs were lysed in l-PPase reaction buffer (New England Biolabs, Ipswich, MA) supplemented with 0.4% NP-40 and EDTA-free protease inhibitors (Sigma-Aldrich). λ-PPase (New England Biolabs) reactions were performed following the manufacturer’s instructions.
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