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Mda adducts elisa kit

Manufactured by Cell Biolabs
Sourced in United States

The MDA Adducts ELISA Kit is a quantitative enzyme-linked immunosorbent assay (ELISA) designed to detect and measure malondialdehyde (MDA) adducts in biological samples. MDA is a reactive aldehyde that can form covalent adducts with proteins, DNA, and other biomolecules, and is commonly used as a biomarker of oxidative stress.

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3 protocols using mda adducts elisa kit

1

Oxidative Stress Measurement Protocol

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To determine the influence of ACE-Is and Pro on oxidative stress parameters, the concentrations of hydrogen peroxide (H2O2) and malondialdehyde (MDA) were measured in culture supernatant with commercially available kits (Hydrogen Peroxide Colorimetric Detection Kit, Assay Designs, USA; MDA Adducts ELISA Kit, Cell Biolabs, USA). Moreover, the amounts of mRNA of NADPH oxidase and SOD were measured in supernatant using the real-time PCR technique.19 ,20 (link) The following PCR primers were designed: NADPH oxidase (5’-CATCCAGCTGTACCTCAGTC-3’ and 5’-GAAAGACTCTTTATTGTATTG-3’) and SOD (5’-GGAAACGCTGGAAGTCGTTTG-3’ and 5’-CTCACTA CAGGTACTTTAAAG-3’).
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2

Evaluation of Hemostasis and Oxidative Stress

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Pro (Plofed 1%, Polfa S.A., Poland), Quin (Accupro, Pfizer, Germany), lipofundin (MCT/LCT 10%, Braun, Germany), gum arabic (Pharma Cosmetic, Poland), pentobarbital (Vetbutal, Biovet, Poland), collagen type I (Collagen, Chronolog, USA), and heparin (Heparinum, Polfa, Poland) were used in the study. Bovine albumin, apyrase, HEPES (N-(2-hydroxyethyl)piperazine-N′-(2-ethanosulfonic acid) were delivered by Sigma-Aldrich (Poland). Calcium chloride, EDTA, glucose, magnesium chloride, sodium chloride, potassium chloride, sodium bicarbonate, sodium phosphate, Tris buffer, and trisodium citrate were provided by Polish Chemicals Reagents (Poland). Rabbit anti-rat TAFI monoclonal antibody (ImmunoKontact AMS Biotechnology, Germany), rat active PAI-1 ELISA kit (Innovative Research, USA), rat active t-PA ELISA kit (Innovative Research, USA), hydrogen peroxide colorimetric detection kit (Assay Designs, USA), correlate assay nitric oxide NO-2/NO-3 assay kit (Assay Designs, USA), MDA adducts ELISA kit (Cell Biolabs, USA), Trizol (Invitrogen Life Technologies, USA), qPCRTM Mastermix, SYBR Green I (Eurogentec Seraing, Belgium), Oligotex Kit (Qiagen, USA), and TaqMan reverse transcription reagents kit (Applied Biosystems, USA) were also used.
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3

Assaying Oxidative Stress Markers

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The markers of oxidative stress, namely hydrogen peroxide (H2O2) and malonyl dialdehyde (MDA) levels, were assayed in plasma by using commercially available kits (Hydrogen Peroxide Colorimetric Detection Kit; Assay Designs and MDA Adducts ELISA Kit; Cell Biolabs, San Diego, CA, USA, respectively).
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