Mda adducts elisa kit

To determine the influence of ACE-Is and Pro on oxidative stress parameters, the concentrations of hydrogen peroxide (H₂O₂) and malondialdehyde (MDA) were measured in culture supernatant with commercially available kits (Hydrogen Peroxide Colorimetric Detection Kit, Assay Designs, USA; MDA Adducts ELISA Kit, Cell Biolabs, USA). Moreover, the amounts of mRNA of NADPH oxidase and SOD were measured in supernatant using the real-time PCR technique.^{19 ,20 (link)} The following PCR primers were designed: NADPH oxidase (5’-CATCCAGCTGTACCTCAGTC-3’ and 5’-GAAAGACTCTTTATTGTATTG-3’) and SOD (5’-GGAAACGCTGGAAGTCGTTTG-3’ and 5’-CTCACTA CAGGTACTTTAAAG-3’).

Pro (Plofed 1%, Polfa S.A., Poland), Quin (Accupro, Pfizer, Germany), lipofundin (MCT/LCT 10%, Braun, Germany), gum arabic (Pharma Cosmetic, Poland), pentobarbital (Vetbutal, Biovet, Poland), collagen type I (Collagen, Chronolog, USA), and heparin (Heparinum, Polfa, Poland) were used in the study. Bovine albumin, apyrase, HEPES (N-(2-hydroxyethyl)piperazine-N′-(2-ethanosulfonic acid) were delivered by Sigma-Aldrich (Poland). Calcium chloride, EDTA, glucose, magnesium chloride, sodium chloride, potassium chloride, sodium bicarbonate, sodium phosphate, Tris buffer, and trisodium citrate were provided by Polish Chemicals Reagents (Poland). Rabbit anti-rat TAFI monoclonal antibody (ImmunoKontact AMS Biotechnology, Germany), rat active PAI-1 ELISA kit (Innovative Research, USA), rat active t-PA ELISA kit (Innovative Research, USA), hydrogen peroxide colorimetric detection kit (Assay Designs, USA), correlate assay nitric oxide NO-2/NO-3 assay kit (Assay Designs, USA), MDA adducts ELISA kit (Cell Biolabs, USA), Trizol (Invitrogen Life Technologies, USA), qPCR^TM Mastermix, SYBR Green I (Eurogentec Seraing, Belgium), Oligotex Kit (Qiagen, USA), and TaqMan reverse transcription reagents kit (Applied Biosystems, USA) were also used.