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Elecsys hbeag

Manufactured by Roche
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Sourced in Germany

The Elecsys HBeAg is a laboratory testing product manufactured by Roche. It is used to detect the presence of the hepatitis B e antigen (HBeAg) in patient samples. The Elecsys HBeAg assay provides a quantitative measurement of HBeAg levels, which can be used to aid in the diagnosis and monitoring of hepatitis B virus infection.

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Lab products found in correlation

Elecsys hbsag 2 quant 2, by Roche (2 mentions) Elecsys hbsag 2, by Roche (2 mentions) Cobas e801, by Roche (1 mentions) Cobas e411 analyzer, by Roche (1 mentions) Cobas ampliprep cobas taqman hbv test, by Roche (1 mentions) Elecsys hbsag 2 assay, by Roche (1 mentions)

5 protocols using elecsys hbeag

1

Quantifying HBV Viral Antigens Reliably

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HBsAg concentration in cell lysates and culture supernatants was measured by electrochemiluminescence immunoassay (ECLIA) using the Elecsys HBsAg II quant II on a Cobas e801 instrument (Roche, Mannheim, Germany). Results were expressed in IU/ml. HBeAg concentration in cell lysates and supernatants was measured using the Elecsys HBeAg (Roche, Mannheim, Germany), in accordance with the manufacturer’s instructions. Results were expressed in Sample/Cut off value (S/CO).
The linearity dynamic range of the assay was validated making serial dilutions of serum samples with known HBeAg levels. Furthermore, the possible interference of the intracellular cell lysate in the specificity of the assay was evaluated by challenging serum samples with known HBeAg or qHBsAg levels diluted with non-transfected cell lysates.
Elizalde M.M., Mojsiejczuk L., Speroni M., Bouzas B., Tadey L., Mammana L., Campos R.H, & Flichman D.M. (2022). Molecular and biological characterization of hepatitis B virus subgenotype F1b clusters: Unraveling its role in hepatocarcinogenesis. Frontiers in Microbiology, 13, 946703.
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2

HBsAg and HBeAg Quantification

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The culture supernatant was collected and centrifuged at 13000 rpm for 5 minutes to remove cell debris. HBsAg and HBeAg were measured by Cobas assay following manufacturer’s instruction. The following kits were used in this study: Elecsys HBsAg II (04687787, cobas, Roche) and Elecsys HBeAg (11820583, cobas, Roche) by cobas e 411 analyzer (Roche).
Wu Y.H., Yang Y., Chen C.H., Hsiao C.J., Li T.N., Liao K.J., Watashi K., Chen B.S, & Wang L.H. (2021). Aerobic glycolysis supports hepatitis B virus protein synthesis through interaction between viral surface antigen and pyruvate kinase isoform M2. PLoS Pathogens, 17(3), e1008866.
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3

Quantitative Detection of HBV Markers

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Serum HBV-DNA was quantified by COBAS AmpliPrep-Cobas TaqMan HBV test (Roche, Basel, CH). HBsAg quantification was carried out by Elecsys®HBsAgII assay (Roche, Basel, CH), with a lower limit of detection of 0.05 IU/ml while HBeAg qualitative detection by Elecsys®HBeAg (Roche, Basel, CH).
Piermatteo L., D’Anna S., Bertoli A., Bellocchi M., Carioti L., Fabeni L., Alkhatib M., Frazia S.L., Lichtner M., Mastroianni C., Sanctis G.D., Marignani M., Pasquazzi C., Iapadre N., Parruti G., Cappiello G., Vecchiet J., Malagnino V., Grelli S., Ceccherini-Silbertein F., Andreoni M., Sarmati L., Svicher V, & Salpini R. (2023). Unexpected rise in the circulation of complex HBV variants enriched of HBsAg vaccine-escape mutations in HBV genotype-D: potential impact on HBsAg detection/quantification and vaccination strategies. Emerging Microbes & Infections, 12(1), 2219347.
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4

Quantifying Hepatitis B Viral Markers

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The culture supernatants and cell lysates of the pHBV1.3C-transfected cells were separately obtained from 12-well plates at the indicated time points. Subsequently, the extracellular and intracellular levels of HBsAg and HBeAg were assessed using electrochemiluminescence (ECL) assays with Elecsys HBsAg II quant II (the lower limit of detection is 0.05 IU/mL) (Roche, Mannheim, Germany) and Elecsys HBeAg (the cutoff ≥1.0 is positive) (Roche, Mannheim, Germany) in accordance with the manufacturer’s directions. These experiments were performed at least in triplicate.
Liao F., Xie J., Du R., Gao W., Lan L., Wang M., Rong X., Fu Y, & Wang H. (2023). Replication and Expression of the Consensus Genome of Hepatitis B Virus Genotype C from the Chinese Population. Viruses, 15(12), 2302.
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5

Quantification of Viral Hepatitis Markers

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Secreted HBeAg and HBsAg in culture supernatants were determined by ELISA (Kehua Bio-Engineering, Shanghai, China). Serum HBsAg and HBeAg levels were measured at Renmin Hospital of Wuhan University (Wuhan, China) by electrochemiluminescence immunoassay using the Elecsys HBsAg II and Elecsys HBeAg kits (Roche Diagnostics, Penzberg, Germany) according to the manufacturer’s direction. The sample results are presented in the form of a relative cutoff index (signal to cutoff ratio, S/CO). Extracellular HBV DNA was quantified by qPCR using a HBV viral DNA quantitative fluorescence diagnostic kit (Sansure Biotech, Changsha, China), and serum HBV DNA was measured with HBV-DNA real-time qPCR kit (Fosun Diagnostics, Shanghai, China).
Xu Z., Zhao L., Zhong Y., Zhu C., Zhao K., Teng Y., Cheng X., Chen Q, & Xia Y. (2021). A Novel Mouse Model Harboring Hepatitis B Virus Covalently Closed Circular DNA. Cellular and Molecular Gastroenterology and Hepatology, 13(4), 1001-1017.
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