We processed previously frozen brains in a cryostat in order to obtain 60-μm serial sections covering the entire hippocampus. Subsequently, we mounted tissue sections in PEN membrane covered slides (Life Technologies®). These, were immediately stained with Cresyl Violet, dehydrated with an ethanol series and stored at −80 °C. The DG was divided into dorsal and ventral regions according to previously described morphological criteria14 (link). The dDG and the vDG were delimited with a Palm (Zeiss®) system, and tissue was mechanically collected in separate tubes using a surgical microscope and micro-forceps (Fig. 2 )
Pen membrane covered slides
Manufactured by Thermo Fisher Scientific
Sourced in United States
The PEN membrane covered slides are a type of laboratory equipment designed for sample preparation and analysis. The slides feature a thin polyethylene naphthalate (PEN) membrane that serves as a substrate for samples, enabling efficient sample handling and processing.
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Lab products found in correlation
2 protocols using pen membrane covered slides
1
Hippocampus Segmentation and Cryopreservation
2
Subicular Microdissection from Rat Brains
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Frozen brains were processed in a cryostat (Leica Biosystems, Wetzlar, Germany) to obtain 60 µm serial sections throughout the entire hippocampus using PEN membrane-covered slides (Life Technologies®, Thermo Fisher Scientific, Waltham, MA, USA). Subsequently, slides were stained with Cresyl Violet, dehydrated with an ethanol series, and stored at -80 ºC. A Palm (Zeiss®, Jena, Germany) system was used to delimitate the dorsal subiculum (dSub) and ventral subiculum (vSub), and the tissue was mechanically collected in separate tubes using a surgical microscope (Zeiss®, Jena, Germany) and ophthalmic forceps. The scheme of the rat brain showing the dorsal and ventral hippocampus is shown in figure 1A, and microdissected regions are shown in figure 1B, and figure 1C.
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