Trastuzumab and Bevacizumab drug products were kindly provided from a Hospital Pharmacy Unit. The NIST monoclonal antibody (NISTmAb, lot number 14HB-D-002) reference material, RM 8671 (2.4 mg mL−1) was purchased from The National Institute of Standards and Technology (NIST). Thermo Scientific™ SMART Digest™ kits, magnetic resin option, were obtained from Thermo Fisher Scientific. LC-MS-grade solvents (0.1% (v/v) formic acid in water, 0.1% (v/v) formic acid in acetonitrile, formic acid, acetonitrile, water) were sourced from Fisher Scientific. Tris(2-carboxyethyl)phosphine hydrochloride (TCEP) and guanidine-HCl were obtained from Pierce. All other reagents were purchased from Sigma-Aldrich.
Thermo scientific smart digest kits
Manufactured by Thermo Fisher Scientific
Sourced in United States
Thermo Scientific™ SMART Digest™ kits are a rapid protein digestion solution for mass spectrometry analysis. The kits provide a streamlined workflow to prepare protein samples for liquid chromatography-mass spectrometry (LC-MS) analysis.
Automatically generated - may contain errors
Lab products found in correlation
Formic acid, by Thermo Fisher Scientific (2 mentions)
Lc ms grade solvents, by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Iodoacetamide, by Merck Group (1 mentions)
Tris 2 carboxyethyl phosphine hydrochloride tcep, by Thermo Fisher Scientific (1 mentions)
3 protocols using thermo scientific smart digest kits
1
Quantitative Comparison of Monoclonal Antibodies
2
Monoclonal Antibody Preparation and Analysis
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The NIST mAb was purchased from
The National Institute of Standards and Technology (NIST). USP mAb
001, USP mAb 002, and USP mAb 003 were obtained from USP (https://www.usp.org/ ) as part of USP mAb round-robin study. The remaining mAbs were produced
internally at Symphogen. Thermo Scientific SMART digest kits and associated
low pH digestion buffer were obtained from Thermo Fisher Scientific.
UHPLC MS-grade water and acetonitrile were sourced from Fisher Scientific.
MS-grade (IonHance) difluoroacetic acid (DFA) was purchased from Waters.
Tris(2-carboxyethyl)phosphine hydrochloride (0.5 M, TCEP) and guanidine-HCl
(8 M, GuHCl) were obtained from Pierce.dl -Dithiothreitol
and iodoacetamide were purchased from Sigma. Ultrapure 1 M Tris-HCl
pH 8.0 was purchased from Invitrogen.
The National Institute of Standards and Technology (NIST). USP mAb
001, USP mAb 002, and USP mAb 003 were obtained from USP (
internally at Symphogen. Thermo Scientific SMART digest kits and associated
low pH digestion buffer were obtained from Thermo Fisher Scientific.
UHPLC MS-grade water and acetonitrile were sourced from Fisher Scientific.
MS-grade (IonHance) difluoroacetic acid (DFA) was purchased from Waters.
Tris(2-carboxyethyl)phosphine hydrochloride (0.5 M, TCEP) and guanidine-HCl
(8 M, GuHCl) were obtained from Pierce.
and iodoacetamide were purchased from Sigma. Ultrapure 1 M Tris-HCl
pH 8.0 was purchased from Invitrogen.
3
Optimized Mass Spectrometry Workflow
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AAV5 empty reference material (06-810) from Applied Viromics (Fremont, CA, USA) was kindly provided by Pharmaron. Thermo Scientific™ SMART Digest™ kits, were obtained from Thermo Fisher Scientific (Sunnyvale, CA, USA). LC-MS grade solvents (0.1% (v/v) formic acid (FA) in water, 0.1% (v/v) FA in acetonitrile, FA, acetonitrile, water) and Tris (2carboxyethyl) phosphine hydrochloride (TCEP) were sourced from Fisher Scientific (Dublin, Ireland). All other reagents were purchased from Sigma-Aldrich (Wicklow, Ireland).
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