Hs 5 and hs 27a bone marrow stromal cells

Manufactured by American Type Culture Collection

Sourced in United States

The HS-5 and HS-27A are human bone marrow stromal cell lines. They are adherent, fibroblast-like cells that can be used for in vitro cell culture and experimentation.

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Lab products found in correlation

Hek293t cells, by Thermo Fisher Scientific (2 mentions) Hyclone, by GE Healthcare (2 mentions) Mda mb 231 breast cancer cells, by American Type Culture Collection (2 mentions) Phenol red free dmem, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)

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HS-5 cells (10 citations)

2 protocols using hs 5 and hs 27a bone marrow stromal cells

Cell Culture Conditions for Microscopy

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We cultured HEK 293T cells (Thermo Fisher Scientific, Carlsbad, CA), MDA-MB-231 breast cancer cells (ATCC), and HS-5 and HS-27A bone marrow stromal cells (ATCC) in Dulbecco's modified eagle's medium (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (HyClone; GE HealthCare, Logan, UT). For microscopy, we replaced the culture medium with phenol red-free DMEM (Thermo Fisher Scientific) supplemented with 1% FBS, 0.1 nM estrogen, 1% penicillin/streptomycin, 1% pyruvate, and 2.5% glutamine unless stated otherwise. Cells were cultured at 37°C in an incubator with 5% CO₂.

Xiao A., Gibbons A.E., Luker K.E, & Luker G.D. (2015). Fluorescence Lifetime Imaging of Apoptosis. Tomography, 1(2), 115-124.

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Cell Culture of Cancer and Stromal Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

We cultured HEK 293T cells (Life Technologies, Carlsbad, CA, USA), MDA-MB-231 breast cancer cells (ATCC), and HS-5 and HS-27A bone marrow stromal cells (ATCC) in DMEM (Life Technologies) supplemented with 10% fetal bovine serum (FBS) (HyClone, GE HealthCare, Logan, UT, USA). For microscopy, we replaced culture medium with phenol red free DMEM (Life Technologies) supplemented with 1% FBS, 0.1 nM estrogen, 1% penicillin/streptomycin, 1% pyruvate, and 2.5% glutamine unless stated otherwise. Cells were cultured at 37°C in an incubator with 5% CO₂.

Xiao A., Gibbons A.E., Luker K.E, & Luker G.D. (2015). Fluorescence Lifetime Imaging of Apoptosis. Tomography, 1(2), 115-124.

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