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Mem media

Manufactured by Corning
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MEM media is a cell culture medium designed for the maintenance and growth of various cell types. It provides the necessary nutrients, vitamins, and other components required for cell survival and proliferation. The core function of MEM media is to support the in vitro cultivation of cells.

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Lab products found in correlation

Penicillin, by American Type Culture Collection (2 mentions) Streptomycin, by American Type Culture Collection (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Trypsin edta solution, by Thermo Fisher Scientific (2 mentions) Tissue culture flask, by Corning (2 mentions) L glutamine, by Corning (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Heat inactivated fetal bovine serum, by Thermo Fisher Scientific (1 mentions) Copper 1 acetate, by Merck Group (1 mentions) Methyl iodide, by Merck Group (1 mentions) Zinc acetate, by Merck Group (1 mentions) Ftir spectrometer, by PerkinElmer (1 mentions) Poly maleic anhydride alt 1 octadecene pmao, by Merck Group (1 mentions) 3 dimethylamino 1 propylamine dmapa, by Merck Group (1 mentions) Indium 3 acetate, by Thermo Fisher Scientific (1 mentions) 1 dodecanethiol ddt, by Thermo Fisher Scientific (1 mentions) Dulbecco s phosphate buffered saline dpbs, by Thermo Fisher Scientific (1 mentions) Spectrum 10, by PerkinElmer (1 mentions) 1 octadecene ode, by Merck Group (1 mentions) Rpmi 1640, by Corning (1 mentions)

Close spelling variants of this product

Opti-MEM media α-MEM media DMEM and MEM growth media MEM/alpha media

9 protocols using mem media

1

Culturing Adherent Cell Lines

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All cell lines were maintained in a humidified 5% CO2 environment at 37 °C in tissue culture flasks (Corning) under normoxic conditions. Adherent cells were dissociated using Trypsin-EDTA solution (0.25%, Gibco). A549-NF-κB luciferase cells were cultured as described previously.62 (link) HeLa were cultured in MEM media (Cellgro) supplemented with 10% FBS (Gibco), penicillin (100 I.U./mL, ATCC), and streptomycin (100 µg/mL, ATCC).
Widen J.C., Kempema A.M., Villalta P.W, & Harki D.A. (2016). Targeting NF-κB p65 with a Helenalin Inspired Bis-electrophile. ACS chemical biology, 12(1), 102-113.
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2

Synthesis and Characterization of Quantum Dots

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Copper (I) acetate (97 %), zinc acetate (99 %), oleic acid (90 %), oleylamine (70 %), 1-octadecene (ODE, 90 %), poly(maleic anhydride-alt-1-octadecene) (PMAO) (average Mn 30,000–50,000 as a powder), 3-(dimethylamino)-1-propylamine (DMAPA) (99 %), methyl iodide (99 %), poly-D-lysine, and gelatin were purchased from Sigma-Aldrich. Indium (III) acetate (99.99 %) and 1-dodecanethiol (DDT, 98 %) were purchased from Alfa Aesar. RPMI-1640 and MEM media were from Corning Cellgro. Heat-inactivated fetal bovine serum(FBS) was from Gibco. Fluorescein diacetate/propidium iodide (FDA/PI) was from Invitrogen. Dulbecco’s phosphate-buffered saline (DPBS) was from Fisher Scientific. U-87 MG and HEK-293 cells were ordered from ATCC. Chemicals were used as received without further purification.
The ultraviolet and visible (UV–Vis) spectra of QDs in organic solvents and aqueous solutions were obtained with a UV–Vis spectrometer (UV-2450 from Shimadzu) using cuvettes with a path length of 1 cm. Photoluminescence spectra were acquired using a spectrophotometer (RF-5301PC from Shimadzu). Infrared (IR) spectra of polymers and QDs were collected using a FT-IR spectrometer (Perkin-Elmer Frontier) equipped with attenuated total reflectance and measurement software (Spectrum 10). Particle size measurements of QDs were made using a ZetaPALS dynamic light scattering detector (Brookhaven Instruments Corp.).
Huang L., Liao M., Chen S., Demillo V.G., Dupre S.A., Zhu X., Publicover N.G, & Hunter KW J.r. (2014). A polymer encapsulation approach to prepare zwitterion-like, biocompatible quantum dots with wide pH and ionic stability. Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology, 16(8), 2555.
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3

Tay-Sachs Patient Fibroblast Cultures

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Human fibroblast cell lines from Tay–Sachs patients (GM00221, GM00502, GM11853) and a healthy normal patient (GM05659) were from Coriell Institute for Medical Research (Camden, NJ); GM00221 donor is homozygous for a 4-base-pair insertion at nucleotide 1278 in exon 11 of the HEXA gene (c.1278insTATC), which leads to a premature termination signal. GM00502 donor is a compound heterozygote with one allele having a 4-base-pair insertion at nucleotide 1278 in exon 11 of the HEXA gene and the second allele having a G>C splice mutation in intron 12 (IVS12+1G>C). GM11853 donor is homozygous for a 4-base-pair duplication in exon 11 of the HEXA gene (1274_1277 dupTATC), which leads to a premature termination signal. Cell lines were grown in MEM media (Cellgro, cat. 10-010-CV, Manassas, VA) supplemented with 10% fetal bovine serum (Gemini Bio, cat. 900-108, West Sacramento, CA). Cells were maintained at <80% confluence under standard incubator conditions (5% CO2, 37 °C, and 75% humidity). Cell viability and cell number count were measured with CellTiter-Glo.
Colussi D.J, & Jacobson M.A. (2019). Patient-Derived Phenotypic High-Throughput Assay to Identify Small Molecules Restoring Lysosomal Function in Tay–Sachs Disease. SLAS discovery : advancing life sciences R & D, 24(3), 295-303.
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4

Cell Line Maintenance under Controlled Conditions

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All cell lines were maintained in a humidified 5% CO2 environment at 37 °C in tissue culture flasks (Corning) under normoxic conditions. Adherent cells were dissociated using either Trypsin-EDTA solution (0.25%, Gibco) or TrypLE Express solution (Invitrogen). HL-60, CCRF-CEM, U-87 MG, GBM6, DU-145, and NCI/ADR-RES cells were cultured as described previously.58 (link), 64 , 80 (link) MCF-7 cells (ATCC, HTB-22) were cultured in MEM media (Cellgro) supplemented with 10% FBS (Gibco), bovine insulin (0.01 mg/mL, Sigma), penicillin (100 I.U./mL, ATCC), and streptomycin (100 µg/mL, ATCC). TEX cells73 (link), 74 (link) were cultured in IMDM containing L-glutamine (Cellgro) supplemented with 15% FBS (Gibco), Stem Cell Factor (20 ng/mL, PeproTech), Interleukin-3 (2 ng/mL, PeproTech), penicillin (100 I.U./mL, ATCC), and streptomycin (100 µg/mL, ATCC).
Kempema A.M., Widen J.C., Hexum J.K., Andrews T.E., Wang D., Rathe S.K., Meece F.A., Noble K.E., Sachs Z., Largaespada D.A, & Harki D.A. (2015). Synthesis and Antileukemic Activities of C1-C10-Modified Parthenolide Analogues. Bioorganic & medicinal chemistry, 23(15), 4737-4745.
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5

Bladder Cell Line Cultivation Protocol

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RT4, T24, SVHUC1, UMUC3 cells were obtained from American Type Culture Collection (ATCC). SVHUC1(transformed, SV40 immortalized) cells were cultivated in Ham's F12K (ATCC 30-2004, Manassas, VA. USA) media supplemented with 7% fetal bovine serum (FBS) (S11195, Atlanta Biologicals, Lawrenceville, GA. USA) and 1% penicillin-streptomycin (30-002-Cl) (Corning Cellgro®, Manassas, VA. USA). Bladder cancer cell lines, T24, and RT4 were cultured in McCoy's 5A (Corning), FBS (10%) and antibiotics. UMUC3 were cultured in MEM media (Corning), FBS (10%), sodium pyruvate (1%), non-essential amino acids (0.1%), sodium bicarbonate (2%). The human bladder epithelial cell lines HBLEC (FC-0040 Lot # 00997 & 01177) was purchased from Lifeline Cell Technology (Oceanside, CA) and were cultivated in ProstaLife™ basal media (LM-0017) supplemented with the ProstaLife™ LifeFactors kit (LS-1072) from LifeLine Cell Technology.
Mukherjee N., Cardenas E., Bedolla R, & Ghosh R. (2017). SETD6 regulates NF-κB signaling in urothelial cell survival: Implications for bladder cancer. Oncotarget, 8(9), 15114-15125.
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6

Isolation and Expansion of Human Corneal Mesenchymal Stromal Cells

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Human corneal mesenchymal stromal cells were isolated and expanded as described before.9 (link) In brief, the corneoscleral button obtained from healthy cadaver eyes (kindly provided by Eversight Eye Bank, Ann Arbor, Michigan, USA) were washed 5 times with phosphate-buffered saline (PBS) containing 2× antibiotic–antimycotic and 2× penicillin—streptomycin (Thermo Fisher Scientific, Inc.). After the central button was removed using a trephine, the limbus was cut into 3 segments, which were placed in 2.4 IU of protease (Dispase II, Thermo Fisher Scientific, Inc.) for 1 hour at 37°C. Intact epithelial sheets were then removed from the stroma. The limbal segments were cut into small pieces and used directly for explant culture in alpha Minimum Essential Medium (MEM) media supplemented with 10% fetal bovine serum, 1× L-glutamine, and 1× nonessential amino acid (NEAA) (all Corning, Inc.). Culture media were changed every other day, and cells were subcultured by brief digestion with a cell-dissociation reagent (TrypLE Express, Thermo Fisher Scientific, Inc.) when 80% confluent. Passages 4 to 6 were used for the experiments. All experiments were repeated with mesenchymal stromal cells from 3 donors.
Rouhbakhshzaeri M., Rabiee B., Azar N., Ghahari E., Putra I., Eslani M, & Djalilian A.R. (2018). New ex vivo model of corneal endothelial phacoemulsification injury and rescue therapy with mesenchymal stromal cell secretome. Journal of cataract and refractive surgery, 45(3), 361-366.
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7

MCF7 Cell Line Cultivation Protocol

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The MCF7 cells used in this study are Homo Sapien, female cells with the RRID:CVCL_0031. This cell line has not been authenticated. MCF7 cells were grown in MEM media (Corning) supplemented with 2mM Glutamine (Hyclone SH30034), 1X Penicillin/Strep (100units/mL Penicillin, 100micrograms/mL Streptomycin), and 10% FBS (Sigma). This media is denoted in the text as complete media or Saturated E2. The cell lines TFF1-MS2 and TFF1-MS2-D12 enhancer deletion are derived from this MCF7 parental clone. They were grown in the same conditions.
Rodriguez J., Ren G., Day C.R., Zhao K., Chow C.C, & Larson D.R. (2018). Intrinsic dynamics of a human gene reveal the basis of expression heterogeneity. Cell, 176(1-2), 213-226.e18.
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8

Metabolic Profiling of Cultured Cells

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Cells were cultured in 10-cm culture dishes (purchased from Thermo Fisher Scientific, Waltham, MA) to approximately 70% confluent in MEM media (purchased from Corning, Corning, NY), supplemented with 4% FBS, 1x streptomycin-penicillin, and 2 mM L-glutamine. On the day of the treatment, old media were removed and replaced with glucose-free MEM media (purchased from Corning) containing 2 mM 13C6-glucose (purchased from Cambridge Isotope Laboratories, Tewksbury, MA). Medium was collected at 0 and 24 h to determine the rates of glucose uptake, lactate alanine/glutamate excretion, and the fraction of glucose that was converted to lactate. After 24 h of incubation, media were removed, plates were washed with cold PBS to remove media components, and cells were harvested and collected for metabolomics analysis as described below.
Sreedhar A., Cassell T., Smith P., Lu D., Nam H.W., Lane A.N, & Zhao Y. (2019). UCP2 overexpression redirects glucose into anabolic metabolic pathways. Proteomics, 19(4), e1800353.
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9

Hippocampal Slices Immunoblotting Analysis

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The hippocampal slices were obtained from 8-to 12-week-old C57BL/6 male mice killed by decapitation. The temporal lobes were cut into 350-mm-thick slices with vibratome. The hippocampal slices were then cultured for immunoblotting in MEM media (Corning) and treated with 5-mM GlcNAc and 150-mM UDP-GlcNAc or 50-mg/mL Ab 25e35 for 1 week. Slices were then homogenized in RIPA buffer containing protease inhibitors (Roche Diagnostics). After 2 hours at 4 C, homogenates were centrifuged at 4000 rpm to discard cellular debris. Protein content was determined by Bradford Assay (Sigma-Aldrich). Protein lysates were diluted in Laemli buffer, boiled at 90 C for 5 minutes, and then resolved by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Proteins were transferred onto the nitrocellulose membrane (Bio-Rad), which were blocked with Tris-buffered saline and 0.1% Tween 20% and 10% nonfat dry milk (GE-Healthcare). Membranes were then incubated at 4 C overnight with the following primary antibodies: syntaxin (Abcam), synaptophysin (Millipore), bIII tubulin, and actin (Sigma-Aldrich). Appropriate secondary IgG HRP-conjugated (GE Healthcare) was added for 1 hour, and chemiluminescent detection was performed with ECL Plus advanced (Amersham and GE Healthcare). Quantitative analysis of the signal obtained was performed by Image J software (National Institutes of Health).
Turano E., Busetto G., Marconi S., Guzzo F., Farinazzo A., Commisso M., Bistaffa E., Angiari S., Musumeci S., Sotgiu S, & Bonetti B. (2015). Neurotoxicity and synaptic plasticity impairment of N-acetylglucosamine polymers: implications for Alzheimer's disease. Neurobiology of aging, 36(5).
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