Human recombinant renin inhibitor screening assay kit

Tilapia frame and skin were acquired from a local sea food processing plant (Fortune Life Company, Kaohsiung, Taiwan). Pepsin (from porcine gastric mucosa), papain (from Carica papaya) and bromelain (from pineapple stem) were purchased from Sigma-Aldrich (St. Louis, MO, USA). ACE (from rabbit lung) and its substrate, N-(3-[2-furyl]acryloyl)-phenylalanylglycylglycine (FAPGG) were also obtained from Sigma-Aldrich. Human recombinant Renin Inhibitor Screening Assay Kit was purchased from Cayman Chemicals (Ann Arbor, MI, USA). Other chemicals and reagents used were of analytical grade.

Fresh vegetables, namely ash gourd (BINA CHALKUMRA 1), bitter gourd (BARI KOROLA 1), brinjal (BARI BEGUN 8), Indian spinach (BARI PUISHAK 1), kangkong (BARI GIMAKOLMI 1), okra (BARI DHEROSH 2), ridge gourd (BARI JHINGA 1), snake gourd (BARI CHICHINGA 1), and stem amaranth (BARI DATA 1), were collected from the Bangladesh Agricultural Research Institute (BARI), Gazipur, Bangladesh. The vegetables were dried in the oven at 40 °C, ground into powders, and then stored at −20 °C. Porcine pancreatic α-amylase, porcine PL, rat intestinal acetone powder, acarbose, orlistat, 4-nitrophenyl α-D-glucopyranoside (PNP), N-(3-[2-furyl]acryloyl)-phenylalanyl glycyl glycine (FAPGG), and rabbit lung ACE were purchased from Sigma-Aldrich (St. Louis, MO, USA). A human recombinant renin inhibitor screening assay kit was purchased from Cayman Chemicals (Ann Arbor, MI, USA). Other analytical grade chemical reagents were obtained from Fisher Scientific Company (Oakville, ON, Canada).