To further determine the levels of CD4+IFN-γ+, CD4+IL-4+ T lymphocyte subsets, cells treated in the previous step were fixed with 500 μL of 4% FA for 20 min in dark, ruptured with 1 mL of Permeabilization Wash Buffer (Bio legend). Then the cells were stained with allophycocyanin (APC) conjugated anti-mouse IFN-γ antibody (0.8 μg/sample), phycoerythrin (PE) conjugated anti-mouse IL-4 antibody (0.2 μg/sample) (Biolegend) for 30 min. All samples were stained in triplicate. These samples were analyzed by flow cytometry, and data were analyzed.
Phycoerythrin pe conjugated anti mouse il 4 antibody
Manufactured by BioLegend
Phycoerythrin (PE) conjugated anti-mouse IL-4 antibody is a laboratory reagent used for the detection and quantification of mouse interleukin-4 (IL-4) in various experimental applications. PE is a fluorescent dye that is conjugated to the anti-mouse IL-4 antibody, allowing for the visualization and measurement of IL-4 expression in samples.
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Lab products found in correlation
3 protocols using phycoerythrin pe conjugated anti mouse il 4 antibody
1
Quantification of T Cell Subsets
2
Intracellular Cytokine Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To further determine the levels of CD4 + IFN-γ + , CD4 + IL-4 + T lymphocyte subsets, cells treated in the previous step were xed with 500 μL of 4% FA for 20 min in dark, ruptured with 1 mL of Permeabilization Wash Buffer (Bio legend). Then the cells were stained with allophycocyanin (APC) conjugated anti-mouse IFN-γ antibody (0.8 μg/sample), phycoerythrin (PE) conjugated anti-mouse IL-4 antibody (0.2 μg/sample) (Biolegend) for 30 min. All samples were stained in triplicate. These samples were analyzed by ow cytometry, and data were analyzed.
3
Intracellular Cytokine Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To further determine the levels of CD4 + IFN-γ + , CD4 + IL-4 + T lymphocyte subsets, cells treated in the previous step were xed with 500 μL of 4% FA for 20 min in dark, ruptured with 1 mL of Permeabilization Wash Buffer (Bio legend). Then the cells were stained with allophycocyanin (APC) conjugated anti-mouse IFN-γ antibody (0.8 μg/sample), phycoerythrin (PE) conjugated anti-mouse IL-4 antibody (0.2 μg/sample) (Biolegend) for 30 min. All samples were stained in triplicate. These samples were analyzed by ow cytometry, and data were analyzed.
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