The human anti‐CD74 antibody SP7219 was discovered by Sutro Biopharma (Sutro Biopharma, San Francisco, CA, USA) using ribosome display technology and expressed in Sutro's proprietary XpressCF+ protein production system as previously reported and detailed in supplementary materials and methods, Appendix S1 23 , 24 , 25 . The biotinylated SP7219 (SP9417) was generated by conjugation of SP7219 with NHS‐PEG4‐Biotin (Thermo Fisher Scientific, Grand Island, NY, USA) through primary amine‐based reaction. The Fluorescein‐labeled SP7219 (SP9240) was generated by the conjugation of a NHS‐Fluorescein (5/6‐carboxyfluorescein succinimidyl ester) (Thermo Fisher Scientific) through primary amine‐based reaction.
Nhs fluorescein 5 6 carboxyfluorescein succinimidyl ester
Manufactured by Thermo Fisher Scientific
NHS‐Fluorescein (5/6‐carboxyfluorescein succinimidyl ester) is a fluorescent labeling reagent used for the covalent attachment of fluorescent dyes to proteins and other biomolecules. It functions as an activated ester, reacting with primary amines to form stable amide bonds.
Automatically generated - may contain errors
2 protocols using nhs fluorescein 5 6 carboxyfluorescein succinimidyl ester
1
Antibody Conjugation for CD74 Targeting
2
Functionalized Silica Nanoparticles for VEGF Delivery
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Methanol,
tetraethyl orthosilicate (TEOS,
≥99.0%), triethyl phosphate (TEP, ≥99.8%), calcium nitrate
tetrahydrate (CNT, ≥98%), 3-aminopropyl triethoxysilane (APTES),
albumin–fluorescein isothiocyanate conjugate (FITC albumin), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide
hydrochloride (EDC), N-hydroxysulfosuccinimide sodium
salt (sulfo-NHS), albumin–fluorescein isothiocyanate conjugate
(albuminFITC), and 37% paraformaldehyde (PFA) were all
obtained from Sigma Aldrich. Aqueous ammonia (25%) was purchased form
Carl Roth. Absolute ethanol (EtOH) and phosphate-buffered saline (tablets,
PBS) were purchased from VWR. NHS–Fluorescein (5/6-carboxyfluorescein
succinimidyl ester) was purchased from Thermo Fisher. Human vascular
endothelial growth factor (VEGF165) was purchased from
Miltenyi.
tetraethyl orthosilicate (TEOS,
≥99.0%), triethyl phosphate (TEP, ≥99.8%), calcium nitrate
tetrahydrate (CNT, ≥98%), 3-aminopropyl triethoxysilane (APTES),
albumin–fluorescein isothiocyanate conjugate (FITC albumin), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide
hydrochloride (EDC), N-hydroxysulfosuccinimide sodium
salt (sulfo-NHS), albumin–fluorescein isothiocyanate conjugate
(albuminFITC), and 37% paraformaldehyde (PFA) were all
obtained from Sigma Aldrich. Aqueous ammonia (25%) was purchased form
Carl Roth. Absolute ethanol (EtOH) and phosphate-buffered saline (tablets,
PBS) were purchased from VWR. NHS–Fluorescein (5/6-carboxyfluorescein
succinimidyl ester) was purchased from Thermo Fisher. Human vascular
endothelial growth factor (VEGF165) was purchased from
Miltenyi.
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