2 days prior to dietary treatment, mice were individually housed. Fasting for 24 hrs was accomplished by placing mice in a new cage without food (fasting arena) with ad libitum access to water. Refeeding entailed allowing fasted mice ad libitum access to either normal chow (Diet 7013, Envigo, Madison, WI; Supp. Fig. 1 ), LFD (D12450B, Research diets, New Brunswick, NJ; Supp. Fig.2 ) or HFD (D12492, Research Diets, New Brunswick, NJ; Supp. Fig. 3 ) for 2 hrs. For behavioral experiments, mice underwent the training phase for NOR or NLR prior to refeeding at 23.5 hrs of fasting. Food disappearance was measured as previously described [15 (link)]. In brief, food was placed in a dish inside the fasting arena and the reduction of weight of the dish + food before and after 2 hrs of refeeding was equated to food consumption. Mouse weight was recorded at the time points indicated using an Ohaus Adventurer Pro digital scale (Parsippany, NJ).
Adventurer pro digital scale
Manufactured by Ohaus
Sourced in United States
The Adventurer Pro digital scale is a precision weighing instrument designed for laboratory use. It provides accurate measurements with a readability of up to 0.0001 g. The scale features a high-contrast LCD display for clear readability and easy operation.
Automatically generated - may contain errors
5 protocols using adventurer pro digital scale
1
Fasting and Refeeding Dietary Protocols
2
Fasting and Refeeding Protocol in Mice
3
Fasting Protocol for Behavioral Studies in Mice
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As we have described (22 (link)), 2 days prior to fasting, mice were singly housed. Fasting was initiated by placing mice in a new cage without food but with ad libitum water. Mice were fasted for 24 h starting at 09:00 h. Immediately after the 24 h fasting, behavioral testing, biomarker studies, and/or injection studies were performed. Consequently, all studies occurred during the dark (active) cycle. Mouse weight was recorded immediately before and immediately after fasting using an Ohaus Adventurer Pro digital scale (Parsippany, NJ, USA).
4
High-Fat Diet Induced Metabolic Alterations
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Mice were initially fed a standard chow of NIH-31 modified open formula (Teklad 7013, Madison, WI, USA) containing 18% calories from protein, 6.2% from fat and 45% from carbohydrates. Mice were then transferred to a feed of open source uniform-base diets, for respective studies, containing either 10% calories from fat (low-fat diet [LFD]; D12450B, Research Diets, New Brunswick, NJ, USA) or 60% calories from fat (HFD; D12492, Research Diets, New Brunswick, NJ, USA). Both diets provided 20% calories from protein. Mouse weight was recorded for the respective weeks using an Adventurer Pro digital scale (Ohaus, Parsippany, NJ, USA). Blood glucose testing results were recorded for the respective weeks by fasting mice for 12 h during their light cycle and sampling tail blood. For glyburide studies, blood glucose testing was conducted immediately post-behavior testing with ad libitum access to food. Glucose was quantified by using an AlphaTRAK blood glucose monitoring system (Abbott Laboratories, North Chicago, IL, USA).
5
Longitudinal Weight Monitoring in Mice
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Mice were weighed weekly using the Adventurer Pro digital scale (Ohaus, Parisippany, NJ) as a repeated measure over time.
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