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2 protocols using anti beta actin antibody sc 1615

1

Antibody Panel for Cellular Signaling Analysis

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antiphospho-tyrosine (P-Tyr-1000; #8954), antiphospho-Tyr1234/1235-MET (#3077), MET (#3127; #8198), antiphospho-Tyr1068-EGFR (#3777), EGFR (#2646; #4267), antiphospho-Ser473-AKT (#4060, #9271), AKT (#4691), antiphospho-Thr202/Tyr204-p44/42 MAPK (Erk1/2) (#9101; #4370), p44/42 MAPK (Erk1/2) (#9102, #4695), PRAS40 (#2691), antiphospho-Thr246-PRAS40, 4E-BP1 (#9644), antiphospho-Thr37/46–4E-BP1, SAPK/JNK (#9252), antiphospho-Thr183/Tyr185-SAPK/JNK, ATF-2 (#9226), antiphospho-Thr71-ATF-2, antiphospho-Ser63-c-Jun (#2361), antiphospho-Ser73-c-Jun (#3270), c-Jun (#9165), antiphospho-Ser897-EphA2 (#6347), EphA2 (#6997) and Vimentin (#5741) antibodies were purchased from Cell Signaling Technology (Danvers, MA); anti-Beta Actin antibody (sc-1615) was purchased from Santa Cruz Biotechnology (Dallas, TX). Peroxidase-labeled affinity purified anti-rabbit IgG (074–1506), anti-mouse IgG (074–1806) and anti-goat IgG (14–13-06) antibodies were purchased from KPL (Gaithersburg, MD).
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2

AKT Signaling Pathway Antibody Panel

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Antiphospho-Ser473-AKT (#4060), antiphospho-Thr308-AKT (#2965), total AKT (#4691), AKT1 (#2938), AKT2 (#2964), AKT3 (#8018), antiphospho-Ser136-BAD (#4366), antiphospho-Ser9-GSK3β (#5558), antiphospho-Thr24-FoxO1/-Thr32-FoxO3 (#9464), antiphospho-Ser2448-mTOR (#5536), antiphospho-Ser2481-mTOR (#2974), antiphospho-Thr202/Tyr204-p44/42 MAPK (Erk1/2) (#9101; #4370), total p44/42 MAPK (Erk1/2) (#9102) and cleaved caspase-3 (#9664) antibodies were purchased from Cell Signaling Technology (Danvers, MA); Anti-Beta Actin antibody (sc-1615) was purchased from Santa Cruz Biotechnology (Dallas, TX). Peroxidase-labeled affinity purified anti-rabbit IgG (074–1506), anti-mouse IgG (074–1806), anti-mouse IgM (074–1803) and anti-goat IgG (14-13-06) antibodies were purchased from KPL (Gaithersburg, MD).
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