Cells were cultured into 70% confluence. Lipofectamine 2000 (Life Technologies) was used to perform transfection according to the manufacture’s instruction. Briefly, SALL4 siRNA (Santa Cruz), non-specific siRNA (Santa Cruz), or Lipofectamine 2000 was diluted with serum-free medium, respectively. The diluted Lipofectamine 2000 was then added into the diluted siRNA, and incubated for 20 minutes at room temperature, and then added into the cell medium. Then, cells were incubated at 37°C, 5% CO2 for 6 hours. After that, the medium in each well was replaced by the normal serum-containing medium, and cultured for 24 hours before the following assays.
Sall4 sirna
Manufactured by Santa Cruz Biotechnology
Sourced in United States
SALL4 siRNA is a small interfering RNA (siRNA) targeting the SALL4 gene. siRNAs are used for gene silencing by targeting and degrading specific mRNA transcripts. The SALL4 siRNA can be used to study the function of the SALL4 gene.
Automatically generated - may contain errors
2 protocols using sall4 sirna
1
SALL4 Knockdown Transfection Protocol
2
Knockdown and Overexpression in ICC-9810 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In order to knock down Sall4 or PTEN, or to overexpress Bmi-1, we transfected the siRNA or expressed vector into ICC-9810 cells by using lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instruction. Sall4 siRNA, PTEN siRNA, and nonspecific siRNA were purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA); Bmi-1 expressed vector was purchased from Qiagen NV (Venlo, the Netherlands). The transfected cells were used for further analysis.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!