The animals were anesthetized with intraperitoneal injection of 3% sodium pentobarbital (20 mg kg−1). The rats were placed and fixed in a prone position. A linear incision was made at the level of lumbar spine, L3–L4. Mini-laminectomy was performed with a microscope. The 2-French Fogarty Catheter (Baxter, Irvine, CA, USA) was passed epidurally up to the level of thoracic spine, T6–T7. The actual position of the catheter tip was confirmed with X-ray immediately (Figure 1 ). The experiment proceeded only when the tip of the catheter was at the desired level. The balloon of the Fogarty catheter was then inflated with room air, 0.2 ml, with a 1-ml syringe for 10 min, rather than with saline and micropump.1 (link) Mini-laminectomy was performed without inserting the catheter in the sham-operated group.
2 french fogarty catheter
Manufactured by Baxter
Sourced in United States
The 2-French Fogarty Catheter is a medical device used for various vascular procedures. It features a flexible, tapered catheter with a balloon at the distal end. The core function of this catheter is to occlude or temporarily block blood flow in a targeted blood vessel.
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2 protocols using 2 french fogarty catheter
1
Spinal Cord Compression Model Using Fogarty Catheter
2
Spinal Cord Compression Injury Protocol
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A balloon-induced compression lesion was performed as described previously38 (link). Briefly, rats were anesthetized with 2–3% isoflurane (Forane, Abbott Laboratiores, UK). The skin was incised on the dorsomedian line from Th8 to Th12. The soft tissue and spinous processes of vertebrae Th10 and Th11 were removed. A 2-french Fogarty catheter (Baxter, Irvine, USA) was placed cranially in the epidural space through laminectomy at Th10 vertebrae. The catheter was positioned at the Th8 spinal level. Spinal compression was created by fast balloon inflation with 15 µl of saline for 5 minutes. The catheter was then quickly deflated and removed. The muscles and skin were sutured in anatomical layers. The animals received gentamicin sulfate (Lek Pharmaceutical, Slovenia, 5 mg/kg) for 5 days to prevent infections and carprofen (Rimadyl, Pfizer, USA, 7,5 mg/kg) to reduce postoperative pain. Postoperative care included manual bladder expression twice per day.
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