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Prt4165

Manufactured by Abcam
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PRT4165 is a recombinant protein product. It is intended for use in various research applications, but no further details on its specific function or intended use are provided.

Automatically generated - may contain errors

Lab products found in correlation

N acetylcysteine, by Merck Group (1 mentions) Brefeldin a, by Cayman Chemical (1 mentions) Thapsigargin, by Santa Cruz Biotechnology (1 mentions) Isrib, by Apexbio (1 mentions) Phenformin p7045, by Merck Group (1 mentions) Bay 876, by Merck Group (1 mentions) Sp600125, by Selleck Chemicals (1 mentions) Sb203580, by Selleck Chemicals (1 mentions) A 769662, by LC Laboratories (1 mentions)

2 protocols using prt4165

1

UV-C Irradiation and PRT4165 Treatment

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Cells were irradiated with 10 J/m2 UV-C using a CL-1000 UV cross-linker (UVP) unless stated otherwise. PRT4165 (Abcam) was used at a concentration of 50 µM as described in Ismail et al. (2013) (link).
Gracheva E., Chitale S., Wilhelm T., Rapp A., Byrne J., Stadler J., Medina R., Cardoso M.C, & Richly H. (2016). ZRF1 mediates remodeling of E3 ligases at DNA lesion sites during nucleotide excision repair. The Journal of Cell Biology, 213(2), 185-200.
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2

CRISPR/Cas9-Mediated Knockout of AMPK and ATF4

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Knockout of AMPK α1/α2 and ATF4 in UMRC6 cell line was done by using sgRNAs and CRISPR/Cas9 technology as follows. The sgRNAs were cloned into the lentiviral lentiCRISPR v2 vector. A LentiCRISPR v2 vector containing sgRNAs against ATF4 was described in our previous publication (30 (link)). All constructs were confirmed by DNA sequencing. pGIPZ-shRNAs against BMI1 were obtained from the Functional Genomics Core at The University of Texas MD Anderson Cancer Center. Other reagents were purchased as follows: 2-deoxy-D-glucose (2DG; D6134), metformin (PHR1084), phenformin (P7045), tunicamycin (T7765), and N-acetyl-cysteine (A7250) from Sigma; A-769662 (LC laboratories, A-1803); 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR; ThermoFisher Scientific, A611700); ISRIB (Apexbio, B3699); brefeldin A (Cayman Chemical, #11861); thapsigargin (Santa Cruz, sc-24017A); PRT-4165 (Abcam, ab146003); BAY-876 (Sigma, SML1774); SP600125 (Selleckchem, S1460); and SB203580 (Selleckchem, S1076).
Zhang Y., Shi J., Liu X., Xiao Z., Lei G., Lee H., Koppula P., Cheng W., Mao C., Zhuang L., Ma L., Li W, & Gan B. (2020). H2A monoubiquitination links glucose availability to epigenetic regulation of the endoplasmic reticulum stress response and cancer cell death. Cancer research, 80(11), 2243-2256.
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