Pre-cultures were inoculated to fresh LB medium to an OD600 of 0.1 and let grown at 30°C until the cultures reach an OD600 of 0.6. For imaging, 5 μl of cell culture were placed on object slides and fixated with agar pads. For experiments involving DAPI staining exponentially growing cells were incubated with DAPI in a final concentration of 0.2 μg/ml for 10 min before the cells were placed on agar pads. Imaging was done on an Axio observer A1 (Zeiss) with an α Plan-Fluar 100x/1.45 Oil (Zeiss) and an Evolve EMCCD camera (Photometrics). Images were evaluated using ImageJ (Schneider et al., 2012 (link)).
α plan fluar 100x 1.45 oil
Manufactured by Zeiss
The α Plan-Fluar 100x/1.45 Oil is a high-magnification objective lens manufactured by Zeiss. It has a magnification of 100x and a numerical aperture of 1.45, designed for use with oil immersion. The objective is intended for microscopy applications that require high-resolution imaging.
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Lab products found in correlation
2 protocols using α plan fluar 100x 1.45 oil
1
Cell Imaging and DAPI Staining
2
Microscopy of Bacterial Cell Cultures
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For the microscopy the cell cultures were grown in LB-medium to early exponential (OD600 of around 1) or early transitionary growth phase (OD600 of around 4). Then 5 µl were pipetted on a cover slide. Then an agarose pad (5% agarose in water) was placed on top of the sample. The slide was placed on the microscope. The microscopy was performed with an Axio observer A1 (Zeiss) with an α Plan-Fluar 100x/1.45 Oil (Zeiss). The Evolve EMCCD camera (Photometrics) was used for the images.
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