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Coated 20 mm microwells of 35 mm petri dish

Manufactured by MatTek
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Sourced in United States

The Coated 20-mm microwells of 35 mm petri dish are a laboratory equipment product designed for cell culture applications. The product consists of a 35 mm petri dish containing twenty 20-mm diameter microwells, which are coated to promote cell attachment and growth. This product is intended to provide a standardized and convenient platform for various cell-based experiments and analyses.

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Lab products found in correlation

Jc 10 dye, by Merck Group (2 mentions) Poly l lysine (pll), by Merck Group (2 mentions)

Close spelling variants of this product

35 mm petri dish 35-mm petri dishes 35-mm dish 35 mm dishes Microwell dishes Petri dishes Petri dish

2 protocols using coated 20 mm microwells of 35 mm petri dish

1

Measuring Mitochondrial Membrane Potential

Check if the same lab product or an alternative is used in the 5 most similar protocols
Changes in Ψm were assessed by staining treated cells with JC-10 dye according to the manufacturer’s (Sigma-Aldrich) instructions with modifications as described by Gharbaran et al. [35 (link)]. Five hundred microliters of medium containing cells at 2 × 105 cells/ml were seeded in poly-L-lysine (Sigma-Aldrich)-coated 20-mm microwells of 35 mm petri dish (MatTek Corporation, Ashland, MA, USA) overnight and then treated with the indicated doses of each compound or DSMA, for 48 h. The cells were then incubated in JC-10 dye Loading Solution for 30 min following standard cell culture conditions. An aliquot (250 μl) of the JC-10 dye Loading Solution-medium mix was withdrawn and replaced with 250 μl Assay Buffer B. Cells were immediately imaged.
Shi C., Huang K., Soto J., Sankaran R., Kalia V., Onwumere O., Young M., Einbond L, & Redenti S. (2023). Piperlongumine inhibits proliferation and oncogenic MYCN expression in chemoresistant metastatic retinoblastoma cells directly and through extracellular vesicles. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 161, 114554.
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2

Measuring Mitochondrial Membrane Potential

Check if the same lab product or an alternative is used in the 5 most similar protocols
Changes in Ψm were assessed by staining treated cells with JC-10 dye according to the manufacturer’s (Sigma-Aldrich) instructions with modifications as described by Gharbaran et al. [35 (link)]. Five hundred microliters of medium containing cells at 2 × 105 cells/ml were seeded in poly-L-lysine (Sigma-Aldrich)-coated 20-mm microwells of 35 mm petri dish (MatTek Corporation, Ashland, MA, USA) overnight and then treated with the indicated doses of each compound or DSMA, for 48 h. The cells were then incubated in JC-10 dye Loading Solution for 30 min following standard cell culture conditions. An aliquot (250 μl) of the JC-10 dye Loading Solution-medium mix was withdrawn and replaced with 250 μl Assay Buffer B. Cells were immediately imaged.
Menke J.A., Broner C.W., Campbell D.Y., McKissick M.Y, & Edwards-Beckett J.A. (2001). Computerized clinical documentation system in the pediatric intensive care unit. BMC Medical Informatics and Decision Making, 1, 3.
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