Buthylhydroxyanisole

Bullfrog oil was provided by Asmarana Produtos Naturais (Natal, Brazil). Sodium hydroxide (NaOH), dimethyl sulfoxide (DMSO), potassium hydroxide, diethyl ether, hydrochloric acid (HCl), Tween^® 20, sodium thiosulfate, and sodium carbonate were from VETEC (Rio de Janeiro, Brazil). Miglyol^® 812 was a gift from Sasol (Witen, Germany). Butylhyldroxytoluene (BHT), buthylhydroxyanisole, potassium biphthalate, potassium dichromate, and Wijs solution were purchased from Labsynth (São Paulo, Brazil). Acetic acid, ethanol P.A, sodium bicarbonate, potassium iodide, starch, and chloroform were from Isofar (Rio de Janeiro, Brazil). Sucralose, Tutti-frutti flavoring and Acesulfame K were purchased from Valdequímica (São Paulo, Brazil). Xanthan gum, sodium benzoate and propylparaben were from ViaFarma (São Paulo, Brazil). Phenolphthalein was provided from Biotec Chemicals (Londrina, Brazil). Mucin Type II (Mucin from porcine stomach) and Span^® 80 were from Sigma-Aldrich (São Paulo, Brazil).

Bullfrog adipose tissue was provided by Asmarana Produtos Naturais (Natal, RN, Brazil). Isooctane and BSTFA was purchased from Sigma Aldrich Inc (St. Louis, MO, USA). Sodium hydroxide, n-hexane, potassium hydroxide, diethyl ether, hydrochloric acid, sodium thiosulfate and sodium carbonate were acquired from VETEC (Rio de Janeiro, RJ, Brazil). Butylhyldroxytoluene, buthylhydroxyanisole, potassium biphthalate, potassium dichromate and Wijs solution were purchased from Labsynth (São Paulo, SP, Brazil). Acetic acid, absolute ethyl alcohol, sodium bicarbonate, potassium iodide, starch and chloroform were acquired from Isofar (Rio de Janeiro, RJ, Brazil). Phenolphthalein was provided from Biotec Chemicals (Londrina, PA, Brazil). Ultrapure water was obtained from a Millipore purification system (Milli-Q plus^®, Millipore, St Quentin en Yvelines, France). All chemicals were of reagent grade and used as received.