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A 395

Manufactured by Merck Group
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Sourced in Germany

The A-395 is a laboratory equipment product manufactured by Merck Group. It serves as a multipurpose device for various scientific applications. The core function of the A-395 is to facilitate controlled temperature and environmental conditions for experimental procedures.

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Lab products found in correlation

Hexanoic acid, by Maclin Biochemical Technology (2 mentions) Chaetocin, by Selleck Chemicals (2 mentions) Eed226, by Selleck Chemicals (2 mentions) Mgcl2, by Merck Group (2 mentions) Fluoroshield with dapi, by Merck Group (2 mentions) Hepes, by Merck Group (2 mentions) Nahco3, by Merck Group (2 mentions) Sodium chloride (nacl), by Merck Group (2 mentions) Sucrose, by Merck Group (2 mentions) Potassium chloride (kcl), by Merck Group (2 mentions) Cacl2, by Merck Group (2 mentions) Nah2po4, by Merck Group (2 mentions) Doxorubicin, by Selleck Chemicals (2 mentions) Pci 340510, by Selleck Chemicals (2 mentions) Entinostat ms 275, by Selleck Chemicals (2 mentions) Romidepsin fk228, by Selleck Chemicals (2 mentions) Gsk126, by Merck Group (1 mentions) Gsk343, by Merck Group (1 mentions) Ro 3306, by Merck Group (1 mentions) Vincristine, by Selleck Chemicals (1 mentions)

6 protocols using a 395

1

Chromatin Regulation Assay in Drosophila

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Sucrose (#S0389), NaCl (#S3014), CaCl2 (#C5670), MgCl2 (#M4880), KCl (#P9541), NaH2PO4 (#S3139), NaHCO3 (#S5761), and HEPES (#54457) were from Sigma-Aldrich. Hexanoic acid (Macklin, #H810882) for PER assay was purchased from Macklin. EED226 (5 μM, Selleck, #S8496), Chaetocin (5 μM, Selleck #S8068), and A-395 (10 μM, Sigma-Aldrich, #SML1923) were added to the standard medium. Flies were kept on these foods for 5 days before the assay (change fresh medium every 2 days).
The following antibodies were used: The antibodies against Histone H3 (di methyl K9) (#ab1220), acetyl histone-h3-k27 (#ab4729), Histone H3 (trimethyl K27) (#ab6002, #PTM-5002, #PTM-647RM), Histone H3 (trimethyl K9) (#ab8898), and Histone H3 antibody (#ab1791) were purchased from Abcam and Jingjie PTM BioLab. The secondary antibodies against rabbit (Alexa Fluor 633, #a21071, HRP-linked Antibody, #111-035-003) and mouse (Alexa Fluor 488, #a11001) were purchased from Jackson ImmunoResearch and Invitrogen. Fluoroshield with DAPI (#F6057) was purchased from Sigma-Aldrich.
Yang J., Tang R., Chen S., Chen Y., Yuan K., Huang R, & Wang L. (2023). Exposure to high-sugar diet induces transgenerational changes in sweet sensitivity and feeding behavior via H3K27me3 reprogramming. eLife, 12, e85365.
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2

Chromatin Regulation Assay in Drosophila

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Sucrose (#S0389), NaCl (#S3014), CaCl2 (#C5670), MgCl2 (#M4880), KCl (#P9541), NaH2PO4 (#S3139), NaHCO3 (#S5761), and HEPES (#54457) were from Sigma-Aldrich. Hexanoic acid (Macklin, #H810882) for PER assay was purchased from Macklin. EED226 (5 μM, Selleck, #S8496), Chaetocin (5 μM, Selleck #S8068), and A-395 (10 μM, Sigma-Aldrich, #SML1923) were added to the standard medium. Flies were kept on these foods for 5 days before the assay (change fresh medium every 2 days).
The following antibodies were used: The antibodies against Histone H3 (di methyl K9) (#ab1220), acetyl histone-h3-k27 (#ab4729), Histone H3 (trimethyl K27) (#ab6002, #PTM-5002, #PTM-647RM), Histone H3 (trimethyl K9) (#ab8898), and Histone H3 antibody (#ab1791) were purchased from Abcam and Jingjie PTM BioLab. The secondary antibodies against rabbit (Alexa Fluor 633, #a21071, HRP-linked Antibody, #111-035-003) and mouse (Alexa Fluor 488, #a11001) were purchased from Jackson ImmunoResearch and Invitrogen. Fluoroshield with DAPI (#F6057) was purchased from Sigma-Aldrich.
Yang J., Tang R., Chen S., Chen Y., Yuan K., Huang R, & Wang L. (2023). Exposure to high-sugar diet induces transgenerational changes in sweet sensitivity and feeding behavior via H3K27me3 reprogramming. eLife, 12, e85365.
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3

Synthesis and Characterization of Small-Molecule Inhibitors

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A-395 (SML1923) and A-395N
(SML1879) were
purchased from Sigma-Aldrich. EED226 (HY-101117) was purchased from
MedchemExpress. SAHA (S1047) was purchased from Selleckchem. Recombinant
human TNF-alpha (210-TA-020) was purchased from R&D Systems. UNC5679
(N-(furan-2-ylmethyl)-8-phenylimidazo[1,2-c]pyrimidin-5-amine) was synthesized as previously reported17 (link) to yield the desired product as a white solid
(6.6 mg) (see Supplemental Methods for
additional information).
Turner A.M., Dronamraju R., Potjewyd F., James K.S., Winecoff D.K., Kirchherr J.L., Archin N.M., Browne E.P., Strahl B.D., Margolis D.M, & James L.I. (2020). Evaluation of EED Inhibitors as a Class of PRC2-Targeted Small Molecules for HIV Latency Reversal. ACS Infectious Diseases, 6(7), 1719-1733.
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4

Generation and Characterization of Prostate Cancer Models

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Generation and molecular characterization of the CRPC (16DCRPC), ENZ-resistant AR+ NE-like (42DENZR and 42FENZR) and ENZ-resistant AR-driven (49CENZR and 49FENZR) tumours and cell lines: in brief, hormone-sensitive LNCaP cells serve as the backbone of the ENZ-resistance model, which was established through serial passaging of CRPC xenografts treated with ENZ24 (link),49 (link) (10 mg kg−1 d−1). These cell lines were cultured in RPMI-1640 containing 5% fetal bovine serum (FBS; Gibco A3160701), with the ENZ-resistant cell lines supplemented with 10 μmol l−1 ENZ for all experiments, unless otherwise noted. The LNCaP (ATCC 1740), C4–2 (ATCC 3314), VCaP (ATCC 2876) and NCI-H660 (ATCC 5813) cell lines were acquired from ATCC, LASCPC-1 cells were obtained from O. Witte (UCLA), and SKO (Ptenf/f), DKO (Ptenf/f/Rb1f/f) and TKO (Ptenf/f/Rb1f/f/Tp53f/f) GEMM cell lines were obtained from D. Goodrich (Roswell Park). The LASCPC-1 and NCI-H660 cell lines were cultured in HITES medium and GEMM cell lines were cultured in PrE medium. When indicated, cells were treated with the following inhibitors: GSK126 (Millipore 500580), GSK343 (Sigma SML0766), A-395 (Sigma SML1923) or RO-3306 (Sigma SML0569). Cultures were assessed for mycoplasma monthly, and all cell lines have been authenticated by short tandem repeat profiling.
Davies A., Nouruzi S., Ganguli D., Namekawa T., Thaper D., Linder S., Karaoğlanoğlu F., Omur M.E., Kim S., Kobelev M., Kumar S., Sivak O., Bostock C., Bishop J., Hoogstraat M., Talal A., Stelloo S., van der Poel H., Bergman A.M., Ahmed M., Fazli L., Huang H., Tilley W., Goodrich D., Feng F.Y., Gleave M., He H.H., Hach F., Zwart W., Beltran H., Selth L, & Zoubeidi A. (2021). An androgen receptor switch underlies lineage infidelity in treatment-resistant prostate cancer. Nature cell biology, 23(9), 1023-1034.
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5

HDAC Inhibitor and Chemotherapeutic Agents

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HDAC inhibitors, TSA, Entinostat (MS-275), Romidepsin (FK228) and PCI-340510 as well as chemotherapeutics Doxorubicin and Vincristine were from Selleckchem (Houston, TX 77014 USA). A-395 was from Sigma-Aldrich (Munich, Germany). The compounds were dissolved in dimethylsulfoxide (DMSO) or DEPC-treated H2O, depending on the manufacturer’s instructions, and stored at − 20 °C.
Schmidt O., Nehls N., Prexler C., von Heyking K., Groll T., Pardon K., Garcia H.D., Hensel T., Gürgen D., Henssen A.G., Eggert A., Steiger K., Burdach S, & Richter G.H. (2021). Class I histone deacetylases (HDAC) critically contribute to Ewing sarcoma pathogenesis. Journal of Experimental & Clinical Cancer Research : CR, 40, 322.
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6

CRISPR/Cas9 Knockouts of HDAC1 and HDAC2 in EwS Cell Lines

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HDAC inhibitors, TSA, Entinostat (MS-275), Romidepsin (FK228) and PCI-340510 as well as chemotherapeutics Doxorubicin and Vincristine were from Selleckchem (Houston, TX 77014 USA). A-395 was from Sigma-Aldrich (Munich, Germany). The compounds were dissolved in dimethylsulfoxide (DMSO) or DEPC-treated H 2 O, depending on the manufacturer's instructions, and stored at -20°C. CRISPR/Cas9 knockouts CRISPR/Cas9 knockouts of HDAC1 and HDAC2 in EwS cell lines (SK-N-MC, EW7, CHLA-10) were generated using the lentiCas9-Blast vector (plasmid #52962; Addgene, Watertown, MA, USA) and lentiGuide-Puro (Addgene; plasmid #52963). The sgRNA sequence recommendations were used and synthesized from libraries for optimized sgRNA: Brunello and Brie, designed to maximize Rule Set 2 scores and minimize off-target sites with high CFD scores [27] . For lentiviral packaging the Trans-Lentiviral Packaging Kit (ThermoFisher Scienti c, Braunschweig, Germany) was used according to the manufacturer's instructions.
Schmidt O., Nehls N., Prexler C., Heyking K.v., Groll T., Pardon K., Hensel T., Gürgen D., Henssen A.G., Eggert A., Steiger K., Burdach S., & Richter G. (2021). Class I Histone Deacetylases (HDAC) Critically Contribute to Ewing Sarcoma Pathogenesis.
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