Anti–β-actin (AC15; Sigma-Aldrich), anti-Listeria (ab35132; Abcam), anti-mouse RIPK3 (R4277; Sigma-Aldrich), anti-MLKL (EPR17514; Abcam), anti-phospho-human MLKL (Thr357/Ser358; EPR9514; Abcam), anti-FLAG (M2; Sigma-Aldrich), and anti-GFP (GT859; GeneTex; 9F9.F9; Abcam) were used. FLAG-tagged human RIPK3 and C-terminally FLAG-tagged human MLKL plasmids were gifts from X. Wang (University of Texas Southwestern, Dallas, TX). NBB140-2xFV-VENUS and control VENUS plasmids were gifts from D. Green (St. Jude Research Hospital, Memphis, TN). Plasmids were transfected using TransIT-X2 Reagent (Mirus Bio LLC). Alexa Fluor 488 phalloidin (Thermo Fisher Scientific), DAPI (Calbiochem), GSK’872 (Sigma-Aldrich), NSA (Calbiochem), TNF (PeproTech), Q-VD-OPh (TONBO Biosciences), SMAC mimetic (Brinapant; LC Laboratories), ethidium homodimer III (Biotium), Hoechst 33342 (Thermo Fisher Scientific), DIM (AP20187, Clontech), and disuccinimidyl suberate (Thermo Fisher Scientific) were used.
Q vd oph
Manufactured by Cytek Biosciences
Sourced in United States
Q-VD-OPh is a broad-spectrum caspase inhibitor that can be used to prevent apoptosis in cell cultures. It functions by inhibiting the activity of multiple caspase enzymes, which play a key role in the execution of programmed cell death.
Automatically generated - may contain errors
Lab products found in correlation
Hoechst 33342, by Thermo Fisher Scientific (2 mentions)
Disuccinimidyl suberate, by Thermo Fisher Scientific (1 mentions)
Ethidium homodimer 3, by Biotium (1 mentions)
Gsk 872, by Merck Group (1 mentions)
Tumor necrosis factor (tnf), by Thermo Fisher Scientific (1 mentions)
Anti β actin ac 15, by Merck Group (1 mentions)
Alexa fluor 488 phalloidin, by Thermo Fisher Scientific (1 mentions)
Anti flag m2, by Merck Group (1 mentions)
Transit x2 reagent, by Mirus Bio (1 mentions)
N acetyl l cysteine nac, by Merck Group (1 mentions)
Glibenclamide, by Fujifilm (1 mentions)
Z yvad fmk, by Santa Cruz Biotechnology (1 mentions)
Human tnf α, by Thermo Fisher Scientific (1 mentions)
Imatinib mesylate, by Novartis (1 mentions)
3 protocols using q vd oph
1
Antibody-based Detection of Cell Death
2
Apoptosis Pathway Inhibitors Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Pan-caspase inhibitor (Q-VD-OPh, Tonbo, CA), JNK inhibitor (SP600125, Santa Cruz, CA), N-Acetyl-L-cysteine (NAC) (Sigma-Aldrich, MO), and JC-1 (5,5´,6,6´-tetrachloro-1,1´,3,3´-tetraethyl-benzimidazolyl-carbocyanine-iodide) (Cell Technology, CA) were dissolved in DMSO. SHK was purchased from Sigma-Aldrich and dissolved in water.
3
Antibody Reagents for Cell Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Antibody against Flag tag and CHX were purchased from Sigma-Aldrich (St Louis, MO, USA). Antibody against Myc tag was purchased from Life technologies (Rockville, MD, USA). Antibody against actin and IM-54 was purchased from Merck Millipore (Billerica, MA, USA). Antibodies against Abl (sc-23), phospho Tyr (PY99, sc-7020), caspase-1 p10 (sc-514), S100A8 (sc-8113), S100A9 (sc-8115) and caspase-1 inhibitor (z-YVAD-fmk) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against cleaved caspase-3 and cleaved PARP were purchased from Cell Signaling Technology (Danvers, MA, USA). Blocking antibody against mouse TNF and purified NA/LE hamster IgG1, λ1 isotype control was purchased from BD Biosciences (San Jose, CA, USA). Blocking antibody against mouse IL-1β was purchased from R&D systems. (Minneapolis, MN, USA). Imatinib mesylate was purchased from Novartis (Basel, Switzerland). Human TNF-α was purchased from Peprotech (Rocky Hill, NJ, USA). Q-VD-OPH was purchased from TONBO biosciences (San Diego, CA, USA). Glibenclamide was purchased from Wako (Osaka, Japan).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!