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Anti alix sc 53540

Manufactured by Santa Cruz Biotechnology
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Anti-Alix (sc-53540) is a primary antibody product offered by Santa Cruz Biotechnology. It is a polyclonal antibody that recognizes the Alix protein. Alix is a protein involved in various cellular processes, including endocytosis and the formation of multivesicular bodies. The core function of this antibody is to facilitate the detection and study of Alix in biological samples.

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Lab products found in correlation

Anti tsg101, by Proteintech (1 mentions) Calnexin 10427 2 ap, by Promega (1 mentions) Anti fibronectin sc 8422, by Santa Cruz Biotechnology (1 mentions) Anti smad2 5339, by Cell Signaling Technology (1 mentions) Anti calnexin sc 11397, by Santa Cruz Biotechnology (1 mentions) Anti cd81 exoab cd81a 1, by System Biosciences (1 mentions) Anti tsg101 sc 7964, by Santa Cruz Biotechnology (1 mentions) Anti smad3 9523, by Cell Signaling Technology (1 mentions) Trichloroacetic acid, by Merck Group (1 mentions) Bradford protein assay, by Bio-Rad (1 mentions)

2 protocols using anti alix sc 53540

1

Characterization of Small Extracellular Vesicles

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Follow previous study, the morphology of SPEVs was observed by Transmission Electron Microscopy (TEM) and the size of SPEVs was detected by Nanoparticle Tracking Analysis (NTA). Furthermore, western blotting analysis of Calnexin (10,427–2-AP, Promega, Madison, WI), a negative marker of EVs, and EV markers protein such as anti-Alix (sc-53540, Santa Cruz, CA, USA), anti-Tsg101 (14497-1-AP, Proteintech), anti-CD9 (20597-1-AP, Proteintech), anti-CD81 (66866-1-AP, Proteintech) was used.
Ding N., Zhang Y., Huang M., Liu J., Wang C., Zhang C., Cao J., Zhang Q, & Jiang L. (2022). Circ-CREBBP inhibits sperm apoptosis via the PI3K-Akt signaling pathway by sponging miR-10384 and miR-143-3p. Communications Biology, 5, 1339.
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2

Immunoblotting Analysis of Protein Complexes

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Cells were lysed in RIPA buffer (150 mM NaCl, 1% NP-40, 0.1% SDS, 50 mM Tris [pH 8.0]) supplemented with 1μg/ml Aprotinin, 1mM DTT, 0.2mM PMSF 1μg/ml pepstatin A. The protein concentration was determined by Bradford Protein Assay (BioRad) and equal amount of proteins were resolved by SDS gel electrophoresis, transferred to a nitrocellulose membrane and subjected to a standard immunoblotting protocol. For the analysis of SEC eluted fractions, the proteins were isolated by Trichloroacetic Acid (Sigma-Aldrich) precipitation followed by acetone washes of the protein pellets. Precipitated proteins were re-suspended directly in Laemmli Sample buffer and resolved by SDS gel electrophoresis. The antibodies used were: rabbit polyclonal anti-Aurora A/AIK #3092, rabbit monoclonal anti-SMAD3 #9523, anti-SMAD2 #5339 (Cell Signaling); mouse monoclonal anti-Tsg101 sc-7964, anti-TGFβ RII sc-17791, anti-Fibronectin sc-8422, anti-Perforin 1 sc-373943, anti-HSP70 sc-24, anti-Alix sc-53540 and rabbit polyclonal anti-calnexin sc-11397 (Santa Cruz Biotechnology, Inc.); mouse monoclonal anti-MYCN Ab16898 and rabbit polyclonal anti-TGFβ RI Ab31013 (abcam); rabbit polyclonal anti-CD81 EXOAB-CD81A-1 (System Biosciences); mouse monoclonal anti-Granzyme A #507202 and anti-Granzyme B #674302 (Biolegend).
Neviani P., Wise P.M., Murtadha M., Liu C.W., Wu C.H., Jong A.Y., Seeger R.C, & Fabbri M. (2018). Natural Killer-derived exosomal miR-186 inhibits neuroblastoma growth and immune escape mechanisms.. Cancer research, 79(6), 1151-1164.
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