A 7890B GC system and 7000C mass spectrometer (Agilent Technologies, USA) equipped with an HP-5MS fused silica capillary column was used for metabolomics analysis. We injected 1-μL samples into the GC-MS apparatus with a split ratio of 50:1. Helium was used as the carrier gas with a flow rate at 1 mL/min. The injection temperature was set to 280 °C, the transfer line temperature was 250 °C, and the ion source temperature 230 °C. We set the electron collision ionization to −70 EV and the frequency of acquisition at 20 spectra/s. MS was performed via electrospray ionization with a mass/charge (m/z) full scan range of 50–800.
7000c mass spectrometer
Manufactured by Agilent Technologies
Sourced in United States
The 7000C mass spectrometer is a laboratory instrument designed for the detection and identification of chemical compounds. It utilizes electron ionization and triple quadrupole technology to provide accurate mass analysis and quantification of samples.
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5 protocols using 7000c mass spectrometer
1
Metabolomic Analysis by GC-MS
2
GC-MS Analysis of Metabolites
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A 7000C mass spectrometer coupled with a 7890B GC system (Agilent Technologies) was implemented for metabolite analysis. A quality control (QC) was prepared by pooling 10 μl of each sample. A 1‐μl aliquot of each sample was injected into the GC–MS with a split ratio of 50:1. The samples were separated in an HP‐5MS fused silica capillary column (30 m × 0.25 mm i.d., 0.25 μm film, Agilent J&W Scientific). Helium gas was used as the carrier gas and the flow rate of helium was set to 2.5 ml/min. The oven temperature programming used in the GC separation began at 60°C for 1 min, before being elevated to 300°C at 8°C/min and held for 5 min. The inlet temperature was set to 280°C, the transfer line temperature was 250°C, and the ion source temperature was 230°C. The mass spectrometer was performed via electron ionization (EI) with a mass/charge full scan range of 50–800 (m/z), and the EI voltage was set to 70 eV. Samples were randomly analyzed to eliminate possible artifacts and ensure the validity of the metabolomics data. The stability of the instrument was evaluated after every seven test samples. Representative GC–MS total ion chromatograms (TICs) are displayed in Figure 1 .
3
Multi-residue Pesticide Analysis in Water
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A total of 102 pesticides were analyzed in the water samples collected. The list of compounds along with the corresponding limit of quantification is provided in Table 2. Twentyseven pesticides were determined using stir bar sorptive extraction and gas chromatography coupled to mass spectrometry (GC-MS) following previously validated methodologies (Lacorte et al., 2009; León et al., 2003) . Analyses were conducted using an Agilent 7890A+ gas chromatograph coupled to a 7000C mass spectrometer (Agilent Technologies, Palo Alto, CA, USA) equipped with a TDU/CIS4 injection system (Gerstel, GmbH, Mülheuim a/d Ruhr, The remaining pesticides were analyzed using a fully automated method based on online solid-phase extraction and liquid chromatography-tandem mass spectrometry determination (SPE-LC-MS/MS). Analyses were conducted using an Advance™ UHPLC OLE system coupled to EVOQ Elite mass spectrometer (Bruker Daltonics Inc, Fremon, CA). Sample preconcentration was done on a YMC C18 trap column (30 mm × 2.1 mm i.d., particle size 10 μm), while chromatographic separation was done on a YMC C18 column (100 mm × 2.1 mm i.d., particle size 2 μm) (both from Bruker). Further details on the analytical method used and its performance are published in Quintana et al. (2019) .
4
Quantitative GC-MS Analysis of Compounds
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A 7890B gas chromatograph (GC) machine was utilized in conjunction with a 7000C mass spectrometer (MS) and an HP-5MS fused silica capillary column (Agilent Technologies, CA, United States) for GC-MS analysis. Each 1 μL aliquot of the derivatized solution was processed in splitting operation at a flow rate of 1 mL/min of helium gas through the column (50: 1). The GC temperature protocol started at 60°C for 4 min, then raised by 8°C/min to 300°C for 5 min. The temperatures of the injection, transfer line, and ion source were 280°C, 250°C, and 230°C, respectively. Electrospray ionization was used to record 20 scans per second across 50–800 m/z.
5
Comprehensive GC-MS Analysis of Metabolic Profiles
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The analysis of all samples was performed using a 7000°C mass spectrometer coupled with a 7890 B gas chromatograph system from Agilent Technologies (CA, United States). The separation of serum, BAT, WAT, liver, spleen and kidney samples was performed using an HP-5MS fused silica capillary column. Using helium gas as a carrier, a 1 µL aliquot of the derivative solution was processed in split mode (50:1), with the front inlet purge flow rate set to 3 mL/min and the gas flow rate set to 1 mL/min. The temperatures for the administration, transfer line, and ion source were maintained at 280°C, 250°C, and 230°C, respectively. The GC temperature program started at 60°C for 4 min, followed by an increase to 300°C at a rate of 8°C/min, and then held at 300°C for 5 min. For ionization, the voltage of the electron impact was set to −70 eV, and data acquisition occ1urred at a rate of 20 spectra per second. MS identification was performed using electrospray ionization (ESI) in full scan mode, with a mass-to-charge ratio (m/z) range of 50–800.
We have effectively uploaded the source data from our GC-MS analysis to MetaboLights. The identifier MTBLS8334 has been exclusively assigned to our research project. To access our study, simply follow this link:https://www.ebi.ac.uk/metabolights/MTBLS8334 .
We have effectively uploaded the source data from our GC-MS analysis to MetaboLights. The identifier MTBLS8334 has been exclusively assigned to our research project. To access our study, simply follow this link:
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