Ab45899

Manufactured by Abcam

Ab45899 is a primary antibody product offered by Abcam. It is designed to detect a specific target protein. The product specifications and technical details are available on the Abcam website.

Automatically generated - may contain errors

Lab products found in correlation

Ab9465, by Abcam (1 mentions) Ab180649, by Abcam (1 mentions) Protein extraction reagent, by Thermo Fisher Scientific (1 mentions) Bca quantitative kit, by Beyotime (1 mentions) Df2434, by Affinity Biosciences (1 mentions) Ab14955, by Abcam (1 mentions) Hoechst, by Merck Group (1 mentions) Hrp linked antibodies, by Santa Cruz Biotechnology (1 mentions) Novex ecl chemi substrate, by Thermo Fisher Scientific (1 mentions) Pgc 1α nbp1 04676, by Novus Biologicals (1 mentions) Akt1 2 3 sc 8312, by Santa Cruz Biotechnology (1 mentions) Phospho akt ser473, by Cell Signaling Technology (1 mentions) β actin, by Merck Group (1 mentions)

3 protocols using ab45899

Protein Expression Analysis by Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols

For Western blotting analysis, protein samples were extracted from the cells through protein extraction reagent (Thermo) and the concentration of protein samples was determined by BCA Quantitative Kit (Beyotime). Then, the difference of protein expression was analysed by Western blotting analysis. The antibodies used in this process were as follows: rabbit anti‐Myocardin (DF2434, Affinity), rabbit anti‐MBNL1 (ab45899, Abcam), mouse anti‐ACTN2 (ab9465, Abcam), rabbit anti‐ANP (ab180649, Abcam), rabbit anti‐TNF‐α (#3707, CST), rabbit anti‐p300 (#86377, CST) and rabbit anti‐GAPDH (#97166, CST). GAPDH expression was used as an internal control.

Xu Y., Liang C., Luo Y, & Zhang T. (2020). MBNL1 regulates isoproterenol‐induced myocardial remodelling in vitro and in vivo. Journal of Cellular and Molecular Medicine, 25(2), 1100-1115.

+ Open protocol

+ Expand

Quantifying Mbnl1 in Mouse Brain Regions

Check if the same lab product or an alternative is used in the 5 most similar protocols

Four mice each from WT, BAC-CAG and BACHD groups were selected for the quantification. Three adjacent coronal brain sections (45 um thick) about 200 um postal bregma point were selected and double stained with Mbnl1 (1:1000. ab45899, Abcam) and NeuN antibodies, then count stained with DAPI. Nine images of dorsal striatum and motor sensory cortex of each brain sections were captured at 60x magnificent using the Dragonfly Confocal microscope. All images were taken with the same conditions for Laser and detector settings. We trimmed the top and bottom of the images and took the 5 μm thick trimmed confocal images for Mbnl1 quantification using the Analyze Particles plugin of the Image J Software (NIH). We set the “Size” at “260-Infinity” and “Circularity” to “0.00–1.00” to count only those relatively big Mbnl1 dots inside the nucleus of NeuN+ cells. All NeuN+ cells in focus of each image were counted. About 500 – 600 cells in total in each group were counted for each group.

Gu X., Richman J., Langfelder P., Wang N., Zhang S., Bañez-Coronel M., Wang H.B., Yang L., Ramanathan L., Deng L., Park C.S., Choi C.R., Cantle J.P., Gao F., Gray M., Coppola G., Bates G.P., Ranum L.P., Horvath S., Colwell C.S, & Yang X.W. (2022). Uninterrupted CAG repeat drives striatum-selective transcriptionopathy and nuclear pathogenesis in human Huntingtin BAC mice. Neuron, 110(7), 1173-1192.e7.

+ Open protocol

+ Expand

Immunoblot and Immunofluorescence Assays

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunoblot and immunofluorescence assays were performed following standard procedures, as previously described [40 (link)]. Primary antibodies were: phospho Histone H3 Ser10 (ab14955, Abcam), TOMM20 (11802-1-AP, Proteintech), PGC1-α (NBP1-04676, Novus Biologicals), phospho p38MAPK Thr180/Tyr182 (9211, Cell Signaling), p38MAPK (sc-7972, Santa Cruz Biotechnology) AKT1/2/3 (sc-8312, Santa Cruz Biotechnology), phospho AKT Ser 473 (9271, Cell Signaling), DMPK (sc-134319, Santa Cruz Biotechnology), MBNL1 (ab45899, Abcam) and β-actin (AC-15, Sigma-Aldrich). For western blot detection of primary antibodies, we used HRP-linked antibodies (Santa Cruz Biotechnology) and the detection was performed by chemiluminescence using Novex ECL Chemi Substrate (Thermo Fisher). For immunofluorescence, nuclear DNA was stained with Hoechst (33342, Sigma-Aldrich).

García-Puga M., Saenz-Antoñanzas A., Fernández-Torrón R., de Munain A.L, & Matheu A. (2020). Myotonic Dystrophy type 1 cells display impaired metabolism and mitochondrial dysfunction that are reversed by metformin. Aging (Albany NY), 12(7), 6260-6275.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!