Anti eea1 antibody

To check that the Ag NPs are endocyted by the cells, a FITC conjugation was realized thanks to NH₂ groups associated to NPs. (Figure S1). On milliliter of the Ag NPs suspension was added to a solution of FITC in 0.1 M carbonate–bicarbonate buffer and vortexed for 1 minute, and the vial reaction was covered with aluminum foil and incubated for 2 hours at room temperature. To finish, the Ag NPs were washed with cold PBS. The cell lines were incubated with conjugated FITC Ag NPs for 15 minutes. After the incubation, the cells were washed with cold PBS and fixed as described previously, followed by a fluorescent identification of the early endosome marker EEA1. The cells were incubated for 30 minutes with blocking reagent, then incubated overnight with anti-EEA1 antibody (Abcam), and finally for 1 hour with alexa fluor 568 conjugated secondary antibody (Figure S2). The slides were mounted using mounting medium with DAPI (Vector, Cambridgeshire, UK). Finally, the analysis was performed with confocal microscope (Zeiss LSM710; Carl Zeiss Meditec AG, Jena, Germany).