His-CELF6 plasmid was synthesized by GenePharma (Shanghai, China). To generate GFP-CELF6, His-CELF6 was used as template, and the cloning primers were designed according to the sequence of human CELF6 gene (NCBI Gene ID: 60677). Flag-β-TrCP (#10865) and HA-Ubiquitin (#17608) were purchased from Addgene. All His-CELF6 truncation plasmids were generated by YouBio (Changsha, China). His-CELF6 point mutations were generated by Q5 Site-directed mutagenesis kit (E0552S, New England Biolabs) using His-CELF6 as the templates.
CELF6 siRNA (sc-90308), p21 siRNA (sc-29427), and p53 siRNA (sc-29435) were purchased from Santa Cruz Biotechnology. β-TrCP specific siRNAs were synthesized by RiboBio or purchased from Dharmacon. siRNA sequences were as follows: Skp2 oligo, 5′- GUACAGCACAUGGACCUAUTT-3′; Fbxw7 oligo, 5′- GCCUCCAGGAAUGGCUAAATT-3′; Cdh1 oligo, 5′- UGAGAAGUCUCCCAGUCAGUTT-3′; Cdc20 oligo, 5′- GGAGCUCAUCUCAGGCCAUTT-3′, which were synthesized by GenePharma.
CELF6 siRNA (sc-90308), p21 siRNA (sc-29427), and p53 siRNA (sc-29435) were purchased from Santa Cruz Biotechnology. β-TrCP specific siRNAs were synthesized by RiboBio or purchased from Dharmacon. siRNA sequences were as follows: Skp2 oligo, 5′- GUACAGCACAUGGACCUAUTT-3′; Fbxw7 oligo, 5′- GCCUCCAGGAAUGGCUAAATT-3′; Cdh1 oligo, 5′- UGAGAAGUCUCCCAGUCAGUTT-3′; Cdc20 oligo, 5′- GGAGCUCAUCUCAGGCCAUTT-3′, which were synthesized by GenePharma.