Shank2

Manufactured by Synaptic Systems

Shank2 is a laboratory instrument used for the analysis and characterization of various materials and samples. It is designed to provide accurate and reliable measurements of the mechanical properties of materials, such as hardness, stiffness, and adhesion. The core function of Shank2 is to perform these measurements in a controlled and repeatable manner, enabling researchers and scientists to better understand the behavior and properties of the materials under investigation.

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Lab products found in correlation

2 protocols using shank2

Monoclonal Antibodies for p140Cap and Synaptic Proteins

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Specific mouse monoclonal antibody (Mab) against p140Cap (clone 2A8) was produced at the MBC, University of Torino, as previously described (Di Stefano et al., 2007 (link); Repetto et al., 2013 (link)). The antibodies used are as follows: Dlg4 (Mab, Abcam), Citron-N (rabbit polyclonal, Abcam), Grin2A (rMab from Millipore; rabbit polyclonal from Thermo Scientific), Grin2b (mouse monoclonal antibody, Neuromab), Grin1 rabbit polyclonal (Thermo Scientific), Shank2 (rabbit polyclonal, Synaptic System), Shank3 (rabbit polyclonal, Synaptic System), Snap25 (Mab, Synaptic System), Actin I-19 (rabbit polyclonal, Santa Cruz Biotechnology), Trio (rabbit polyclonal, Santa Cruz Biotechnology), rabbit polyclonal Camk2 (Cell Signaling), Homer-1 (rabbit polyclonal, Thermo Scientific), Cadherin-11 (Mab, Thermo Scientific), Syncam (rabbit polyclonal, Thermo Scientific), Ttc3 (rabbit polyclonal, as described in Berto et al., 2007 (link)), and Gfap (Mab, Dako-Agilent Technologies), Bassoon (rabbit polyclonal, Synaptic System). Mouse and rabbit IgGs were purchased from Santa Cruz Biotechnology. Secondary antibodies anti-mouse and anti-rabbit were purchased from Sigma Aldrich.

Alfieri A., Sorokina O., Adrait A., Angelini C., Russo I., Morellato A., Matteoli M., Menna E., Boeri Erba E., McLean C., Armstrong J.D., Ala U., Buxbaum J.D., Brusco A., Couté Y., De Rubeis S., Turco E, & Defilippi P. (2017). Synaptic Interactome Mining Reveals p140Cap as a New Hub for PSD Proteins Involved in Psychiatric and Neurological Disorders. Frontiers in Molecular Neuroscience, 10, 212.

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Immunocytochemistry and Western Blot Antibodies

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Primary antibodies used for immunocytochemistry and/or western blots included: Homer1 (1:750, Synaptic Systems; 160003), Shank2 (1:1,000, Synaptic Systems; 162204), Shank3 (1:500, Synaptic Systems; 162302 and 162304), GluA1 and GluA2 [1:8 for surface staining and 1:100 for whole cell staining, Millipore; PC246 and MAB397], VGluT1 (1:100, NeuroMab; N28/9), Shank2 (1:100, Neuromab; N23B/6), Shank3 (1:100, NeuroMab; N367/62), PSD-95 (1:100, NeuroMab; K28/43), MAP2 (1:5,000, Abcam; ab5392), green fluorescent protein (1:1,000, Abcam; ab13970), actin (1:1,000, Abcam; ab8227), and Shank2 (1:250, Cell Signaling; 12218). A custom-made VGluT1 antibody (1:500, polyclonal rabbit) was generously provided by Dr. Richard Reimer (Stanford University). All secondary antibodies (1:500, A11029, A11034, A11036, A11039, A11041, A11075, A21235 and A21449) were obtained from Life Technologies with the exception of the Dylight-350 antibody (1:250, Thermo Fisher; SA5-10069) and the HRP-conjugated antibodies (1:10,000, rabbit, mouse or guinea pig; Jackson ImmunoResearch; 706-035-148, 115-035-003 and 111-035-144).

Ha H.T., Leal-Ortiz S., Lalwani K., Kiyonaka S., Hamachi I., Mysore S.P., Montgomery J.M., Garner C.C., Huguenard J.R, & Kim S.A. (2018). Shank and Zinc Mediate an AMPA Receptor Subunit Switch in Developing Neurons. Frontiers in Molecular Neuroscience, 11, 405.

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