Hct116 colon

The following human cancer cell lines were obtained from the Bioevaluation Center at the Korea Research Institute of Bioscience and Biotechnology (KRIBB): HCT116 colon cancer cells, non-small-cell lung cancer adenocarcinoma A549 and NCI-H1975, pancreatic cancer MIA-PaCa-2, prostate cancer PC-3, and renal cancer Caki-1 and 786-O. Cells were cultured in a 5% CO₂ atmosphere at 37 °C in Dulbecco’s modified Eagle’s medium (Gibco, Carlsbad, CA, USA) or RPMI (Gibco) supplemented with 10% fetal bovine serum (Gibco), 100 U/ml penicillin, and 100 μg/ml streptomycin (Gibco). Hypoxic conditions were achieved by placing the cells in a 1% O₂, 94% N₂, and 5% CO₂ multigas incubator (Sanyo, Osaka, Japan).

SW837 rectal cancer and HCT116 colon cancer cell lines were obtained from the European Collection of Cell Culture (ECACC). SW837 cells were maintained in Leibovitz’s (L-15) culture media (Lonza, Basel, Switzerland), supplemented with penicillin-streptomycin (1%) (v/v) (Lonza), foetal bovine serum (FBS) (10%) (v/v) (Gibco, Waltham, MA, USA), and L-Glutamine (Lonza) (1%) (v/v) (complete medium), in non-vented flasks. HCT116 colon cancer cells were maintained in Roswell Park Memorial Institute (RPMI)-1460 medium (Gibco), supplemented with penicillin-streptomycin (1%) (v/v), and FBS (10%) (v/v) (complete medium) (complete RPMI (cRPMI)), in vented flasks. Cell lines were cultured at 37°C, in 5% CO₂/95% humidified air.