Oragene og 500 dna collection storage receptacles

The participants were recruited in years 2010–2012 within research project TESTOPLEK and registered as a POPULOUS collection at the Biobank Lab of The Department of Molecular Biophysics of The University of Lodz [31 ]. Sampling was performed by a professional public opinion polling and survey-taking company (SMG/KRC Poland, a Millward Brown subsidiary). Each subject gave the written informed consent and fulfilled questionnaire. The saliva was collected into Oragene OG-500 DNA collection/storage receptacles (DNA Genotek, Kanata, Canada) from each individual. The approval for this study was obtained from The University of Lodz’s Review Board (KBBN-UL/II/2014). All procedures were performed in accordance with the Declaration of Helsinki (ethical principles for medical research involving human subjects).
From over 10000 individuals throughout Poland, a total of 6047 participants were involved in creation a study group (Fig 1). The exclusion criteria were: diabetes, leukemia, bone marrow transplantation and cancer. Individuals who declared any of these diseases were excluded from the study (n = 488). A total of 5559 subjects declared themselves healthy. The study group consisted of 2812 females and 2747 males.
BMI of each participant was calculated from the standard formula: BMI = weight/height², based on self-assessment of height and weight controlled by trained pollsters.

Control donors were recruited between 2010 and 2012 within the research project TESTOPLEK and registered as the POPOLOUS collection at the Biobank Lab of The Department of Molecular Biophysics of The University of Lodz, Poland. Each donor gave written, informed consent to participate. Saliva was collected into Oragene OG-500 DNA collection/storage receptacles (DNA Genotek, Kanata, Canada) from each individual. The approval for this study was obtained from The University of Lodz’s Review Board (32/KBBN-UL/I/2018). All procedures were performed in accordance with the Declaration of Helsinki (ethical principles for medical research involving human subjects) [30 (link),31 (link)].
From over 6047 adult individuals throughout Poland, a total of 5606 participants were involved in a matched control group of the study. The exclusion criteria were diabetes, myeloid disorders, bone marrow transplantation, and cancer. There were 2860 (51%) females and 2746 (49%) males, aged from 22 to 77 years (42.86 ± 14.85 and 42.03 ± 14.72, respectively).
Based on mitochondrial DNA (mtDNA) studies Poles can be considered as genetically homogenous, with the same pattern as other population within European countries (e.g., The Netherlands, Austria, Spain, Portugal, Sardinia and Russia) [32 (link)].