Percp conjugated cd45

Manufactured by BD

PerCP-conjugated CD45 is a fluorescent-labeled antibody that binds to the CD45 cell surface antigen. CD45 is a transmembrane protein tyrosine phosphatase expressed on the surface of most hematopoietic cells. The PerCP fluorescent dye allows the detection and analysis of CD45-positive cells using flow cytometry.

Automatically generated - may contain errors

Lab products found in correlation

Permeabilization buffer, by BD (2 mentions) Fitc conjugated 1 ab, by BD (2 mentions) Pe conjugated cd11c, by BD (2 mentions) Apc conjugated f4 80 and epcam, by BD (2 mentions) Pe conjugated il 33, by R&D Systems (2 mentions) 5 laser facs aria 2, by BD (2 mentions) Accuri c6 cytometer, by BD (2 mentions) Cytofix cytoperm, by BD (2 mentions) Fitc conjugated anti ly6c, by BioLegend (1 mentions) Pe conjugated anti ly6g, by BD (1 mentions) Pe cy7 conjugated anti cd11b, by BD (1 mentions) Facscanto 2 analyzer, by BD (1 mentions) Apc conjugated cd34, by BD (1 mentions) Fitc conjugated cd15, by BD (1 mentions) Fitc conjugated cd14, by BD (1 mentions) Facsdiva software, by BD (1 mentions) Fitc conjugated cd34, by BD (1 mentions) Tryple select, by Thermo Fisher Scientific (1 mentions) Facscanto, by BD (1 mentions) Trucount tube, by BD (1 mentions)

5 protocols using percp conjugated cd45

Cardiac Leukocyte Characterization by Flow Cytometry

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total cardiac cells were gated on PerCP-conjugated CD45 (BD Biosciences), and the following antibodies were used: PE-Cy7–conjugated anti-CD11b (BD Pharmingen), PE-conjugated anti-Ly6G (lymphocyte antigen 6 complex, locus G; 1A8; BD Pharmingen), FITC-conjugated anti-Ly6C (lymphocyte antigen 6 complex, locus C; Biolegend), PE-Alexa700–conjugated anti-CD3 (eBioscience) and APC-conjugated anti-F4/80 (Bio-Rad). CD45⁺/CD11b⁺ leukocytes were gated on F4/80⁻ monocytes and then further stratified by Ly6G and Ly6C expression. Monocytes were identified as CD11b^highLy6G⁻Ly6C⁺. Neutrophils were identified as CD11b⁺Ly6G^high and T cells as CD45⁺CD3⁺. The total number of cells was then normalized to LV weight. Cells were analyzed using a flow cytometer (LSR II; BD Biosciences).^{10 (link),11 (link)}

Loyer X., Zlatanova I., Devue C., Yin M., Howangyin K.Y., Klaihmon P., Guerin C.L., Kheloufi M., Vilar J., Zannis K., Fleischmann B.K., Hwang D.W., Park J., Lee H., Menasché P., Silvestre J.S, & Boulanger C.M. (2018). Intra-Cardiac Release of Extracellular Vesicles Shapes Inflammation Following Myocardial Infarction. Circulation Research, 123(1), 100-106.

+ Open protocol

+ Expand

Immunophenotyping of Trophoblast Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

TNCs (2 × 10⁵) were labeled with a combination of PerCP‐conjugated CD45 (BD Biosciences, clone 2D1) and APC‐conjugated CD34 (BD Biosciences, clone 8G12) plus: FITC‐conjugated CD14 (BD Biosciences, clone MSE2) for monocyte detection; PhycoErythrin (PE)‐conjugated CD56 (BD Biosciences, clone MY31), FITC‐conjugated CD2 and CD3 (BD Biosciences, clone MOPC21 and UCHT1), FITC‐conjugated CD19 and CD20 (BD Biosciences, clone HIB19 and 2H7) for lymphocyte detection; and FITC‐conjugated CD15 (BD Biosciences, clone MMA) for granulocyte detection. The percentage of cell impurities was calculated as the percentage of CD45⁺ events after the exclusion of CD34⁺ events using a FACS Canto II analyzer (BD Biosciences) and FACS DIVA software (Supporting Information Fig. S2).

Saucourt C., Vogt S., Merlin A., Valat C., Criquet A., Harmand L., Birebent B., Rouard H., Himmelspach C., Jeandidier É., Chartois‐Leauté A., Derenne S., Koehl L., Salem J., Hulot J., Tancredi C., Aries A., Judé S., Martel E., Richard S., Douay L, & Hénon P. (2019). Design and Validation of an Automated Process for the Expansion of Peripheral Blood‐Derived CD34+ Cells for Clinical Use After Myocardial Infarction. Stem Cells Translational Medicine, 8(8), 822-832.

+ Open protocol

+ Expand

Lung Leukocyte Phenotyping in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

The phenotype of cells from mouse lungs was determined by incubating lung leukocytes with the indicated antibodies and CD16/32 to limit nonspecific binding. Leukocytes were stained at 4°C for 15 min in PBS containing 1%BSA and 0.01% sodium azide. Cells were stained with combinations of the following antibodies: FITC-conjugated I-A^b; PE-conjugated CD11c; PerCP-conjugated CD45; and APC-conjugated F4/80 and EpCAM from BD Biosciences. For intracellular IL-33 staining, cells were incubated with Cytofix/Cytoperm (BD Biosciences, San Diego, CA), washed in Permeabilization Buffer (BD Biosciences), and stained for 30 min with PE-conjugated IL-33 (R&D systems, Minneapolis, MN). Cells were washed and resuspended in 1% paraformaldehyde. Isotype controls were used. Data was acquired using BD Accuri™ C6 cytometer and analyzed using FCS Express 4.0 Software. For cell sorting experiments, F4/80⁺ leukocytes from the lungs of WT and CCR2^−/− mice were isolated at day-7 p.i. using 5-laser FACS Aria II (BD Biosciences).

Verma A., Kroetz D.N., Tweedle J.L, & Deepe GS J.r. (2014). Type II Cytokines Impair Host Defense Against Intracellular Fungal Pathogen by Amplifying Macrophage Generation of IL-33. Mucosal immunology, 8(2), 380-389.

+ Open protocol

+ Expand

Pluripotency and Hematopoietic Marker Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

For analysis of pluripotency markers, hiPSCs were dissociated into single cells using TrypLETM Select (Gibco), blocked with 10% human AB serum, stained with FITC-conjugated SSEA-3, PE-conjugated SSEA-4, and Alexa Fluor 647- conjugated TRA-1-60 (BioLegend, San Diego, CA, United States), and analyzed by BD FACS Canto (BD Biosciences, Franklin Lakes, NJ, United States).
For immunophenotypic analysis of the differentiated HSPCs, cells were resuspended in FACS buffer (PBS with 2% BSA), incubated with antibody cocktail for 30 min at 4°C in the dark, and analyzed by BD FACS Canto. The antibodies used in this study included FITC-conjugated CD34, PerCP-conjugated CD45, PE-Cy7-conjugated CD43 (BD Biosciences), and APC-conjugated CD235a (glycophorin A, GPA) (BioLegend). Absolute counts were performed using BD Trucount tubes containing a known quantity of fluorescent beads.

Luanpitpong S., Tangkiettrakul K., Kang X., Srisook P., Poohadsuan J., Samart P., Klaihmon P., Janan M., Lorthongpanich C., Laowtammathron C, & Issaragrisil S. (2024). OGT and OGA gene-edited human induced pluripotent stem cells for dissecting the functional roles of O-GlcNAcylation in hematopoiesis. Frontiers in Cell and Developmental Biology, 12, 1361943.

+ Open protocol

+ Expand

Lung Leukocyte Phenotyping in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!