Ferritin antibody for immunofluorescence

HepG2 cell line was obtained from HonorGene (Changsha, China). Ferrostatin-1, MTT, 3-methyladenin (3-MA), chloroquine (CQ), RPMI-1640, and other chemicals were purchased from Sigma-Aldrich. 2-Pyridylhydrazone dithiocarbamate s-acetic acid (PdtaA) was prepared in our laboratory [27 (link)]. GPx4, xCT (SLC7A11), NDRG1, vimentin, and NCOA4 antibody were obtained from the Proteintech Group (Wuhan, China). Antibodies of E-cadherin and secondary antibodies (fluorescence-labeled for immunofluorescence) were purchased from Cell Signaling Technology (USA). Ferritin antibody for immunofluorescence was obtained from Santa Cruz Biotechnology (USA, Santa Cruz). NCOA4 antibody for immunofluorescence was purchased from Atlas Antibody (Sweden). Secondary antibodies for Western blotting were obtained from EarthOx, LLC (San Francisco, USA).

3-Methyladenine (3-MA), dichlorofluorescein (H₂DCF-DA), ferrostatin-1, 4′,6-diamidino-2-phenylindole (DAPI), TGF-β1, N-acetyl-L-cysteine (NAC), Roswell Park Memorial Institute- (RPMI-) 1640, and other chemicals were purchased from Sigma-Aldrich (USA). Erastin was from MedChemExpress. Antibodies of vimentin, NCOA4, Nrf2, Keap1, HO-1, and gapdh for Western blotting were obtained from Proteintech Group Inc. (Wuhan, China). Antibodies of E-cadherin and ferritin (H chain) and secondary antibodies (fluorescence labeled for immunofluorescence) were purchased from Cell Signaling Technology (USA). Ferritin antibody for immunofluorescence was obtained from Santa Cruz Biotechnology (USA, Santa Cruz). NCOA4 antibody for immunofluorescence was purchased from Atlas Antibody (Sweden). Secondary antibodies for Western blotting were obtained from EarthOx, LLC (San Francisco, USA).

The HepG2 cell line was obtained from HonorGene; Changsha Aibiwei Biotechnology Co., Ltd. Ferrostatin-1, MTT, di-2-pyridylketone, 3-methyladenin (3-MA), RPMI-1640, and other chemicals were purchased from Sigma-Aldrich; Merck KGaA. GPx4, xCT (SLC7A11), vimentin, nuclear receptor coactivator-4 (NCOA4), and LC3 antibodies were obtained from ProteinTech Group, Inc. Antibodies against p53, caspase-8, GAPDH, Bax, and Bcl-2 were purchased from Wuhan Boster Biological Technology, Ltd. Antibodies targeting E-cadherin and secondary antibodies (fluorescence-labeled for immunofluorescence) were purchased from Cell Signaling Biotechnology, Inc. Ferritin antibody for immunofluorescence was obtained from Santa Cruz Biotechnology, Inc. NCOA4 antibody for immunofluorescence was purchased from Atlas Antibodies. Secondary antibodies for western blotting were obtained from EarthOx Life Sciences.