Acl top 700 analyzer

Manufactured by Werfen

Sourced in United States, Germany, Spain

The ACL TOP‐700 analyzer is a laboratory instrument designed for the analysis of coagulation parameters. It is capable of performing a wide range of coagulation tests, including prothrombin time (PT), activated partial thromboplastin time (aPTT), and fibrinogen levels. The analyzer provides reliable and consistent results, with high-throughput capabilities to support the needs of clinical laboratories.

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Lab products found in correlation

Fluoroskan ascent fluorometer, by Thermo Fisher Scientific (1 mentions) Calibrated automated thrombogram, by Diagnostica Stago (1 mentions) Hemosil von willebrand factor antigen kit, by Werfen (1 mentions) Hemosil fviii deficient plasma, by Werfen (1 mentions) Hemosil synthasil, by Werfen (1 mentions) Hemosil d dimer hs 500, by Werfen (1 mentions) Alteplase, by Boehringer Ingelheim (1 mentions) Liquid anti xa, by Werfen (1 mentions) Readiplastin, by Werfen (1 mentions) Cobas 8000 modular, by Roche (1 mentions) C702 module, by Roche (1 mentions) Cobas 8000, by Roche (1 mentions)

8 protocols using acl top 700 analyzer

Apolipoprotein and Coagulation Factors Profiling

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Levels of apolipoprotein (apo) A-I, A-II, A-IV, (a), B-100, B-total, C-I, C-II, C-III and E were measured on unthawed serum samples. To determine the apolipoprotein profile, a mass spectrometric method was developed for multiplexed quantification of apolipoproteins [14 (link),19 (link)].
All coagulation-related laboratory measurements (FVII, FVIII, FIX, FXI, vWF:Ag) were analyzed on the ACL-Top 700 Analyzer (Instrumentation Laboratory). FVIII, FIX, and FXI levels were measured using modified activated partial thromboplastin time assays with immunodepleted plasmas. Similarly, FVII was determined using a modified prothrombin time assay. vWF antigen was measured using an automated latex–enhanced immunoassay with the HemosIL vWF:Ag Reagent Kit [10 (link)].
The thrombin generation potential was assessed by the Calibrated Automated Thrombogram (Diagnostica Stago) according to the specifications of the manufacturer [20 (link)]. The fluorescent signal representing generated thrombin was monitored in a Fluoroskan Ascent fluorometer (Thermo Fisher Scientific), and the parameters were calculated with the Thrombinoscope software (Thrombinoscope BV). Endogenous thrombin potential (ETP) was the parameter determined through the thrombin generation assay [11 (link),15 (link)].

Camilleri E., van Rein N., van Vlijmen B.J., Biedermann J.S., Kruip M.J., Leebeek F.W., van der Meer F.J., Cobbaert C.M., Cannegieter S.C, & Lijfering W.M. (2023). Influence of rosuvastatin on apolipoproteins and coagulation factor levels: Results from the STAtin Reduce Thrombophilia trial. Research and Practice in Thrombosis and Haemostasis, 7(2), 100063.

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Platelet Contribution to Coagulation Factors

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Coagulation assays encompassing APTT, PT, as well as fibrinogen, the coagulation factors XII, VIII, and VII in plasma, and PRP were analyzed with the Instrumentation Laboratory ACL TOP 700 analyzer (Instrumentation Laboratory, Bedford, MA). The assays for VWF (von Willebrand factor) antigen, VWF collagen binding (CB), and VWF ristocetin cofactor assays were performed on an ACL AcuStar instrument (Instrumentation Laboratory, Bedford, MA).
To explore the contribution of platelets to blood coagulation and coagulation factor activity, the standard protocol of coagulation assays was modified using PRP instead of plasma. To further assess the contribution of platelets to the activity of coagulation factors, in a subset of COVID-19 patients, washed platelets were suspended in the corresponding volume of plasma obtained from pooled plasma samples of healthy controls and tested in the assays of coagulation factors. References were the activities of coagulation factors measured in plasma and PRP of healthy subjects.

Taus F., Salvagno G., Canè S., Fava C., Mazzaferri F., Carrara E., Petrova V., Barouni R.M., Dima F., Dalbeni A., Romano S., Poli G., Benati M., De Nitto S., Mansueto G., Iezzi M., Tacconelli E., Lippi G., Bronte V, & Minuz P. (2020). Platelets Promote Thromboinflammation in SARS-CoV-2 Pneumonia. Arteriosclerosis, Thrombosis, and Vascular Biology, 40(12), 2975-2989.

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Factor VIII Activity and Inhibitor Measurement

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The one‐stage‐based activated partial thromboplastin time assay (Instrumentation Laboratory, Bedford, MA, USA) was used to measure FVIII activity in a multi‐dilution mode, with FVIII‐deficient plasma. The Nijmegen modification of the Bethesda Assay was used for the determination of FVIII inhibitors. An ACL TOP‐700 analyzer was used to perform all measurements (Instrumentation Laboratory, Bedford, MA, USA). A latex particle‐enhanced immunoturbidimetric assay with HemosIL von Willebrand factor antigen kit (Instrumentation Laboratory, Bedford, MA, USA) was utilized to determine VWF:Ag levels.

Chen Z., Huang K., Li G., Zhen Y., Wu X., Di A., Liu G., Li Z., Alfonso I, & Wu R. (2021). Pharmacokinetic variability of factor VIII concentrates in Chinese pediatric patients with moderate or severe hemophilia A. Pediatric Investigation, 5(1), 38-45.

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Coagulation Factor VIII Activity Assessment

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The one‐stage–based activated partial thromboplastin time (aPTT) assay was used to measure FVIII activity in a multidilution mode, with HemosIL FVIII deficient plasma (Instrumentation Laboratory, Bedford, MA, USA). The aPTT reagent was HemosIL SynthAsil (Instrumentation Laboratory). The Nijmegen modification of the Bethesda assay was used for determining the levels of FVIII inhibitors. von Willebrand factor antigen (VWF:Ag) level were detected with a latex particle‐enhanced immunoturbidimetric assay using the HemosIL VWF:Ag kit (Instrumentation Laboratory). The ACL TOP‐700 analyzer (Instrumentation Laboratory) was used to perform all measurements.

Huang K., Zhen Y., Li G., Wu X., Chen Z, & Wu R. (2022). Enhanced pharmacokinetics and reduced bleeds in boys with hemophilia A after switching to Kovaltry from other standard half‐life factor VIII concentrates. Research and Practice in Thrombosis and Haemostasis, 6(2), e12686.

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Thrombolysis Assay with DNase 1

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Thrombi were cut in two equal parts and treated with or without DNase 1 (100 µg/ml, Dornase Alfa, Pulmozyme; Roche, Basel, Switzerland) at 37°C under agitation (500 rpm, Thermomixer) in phosphate buffered saline supplemented with Glu‐plasminogen (1 µM, Technoclone; Vienna, Austria) and tPA (10 µg/ml Alteplase, Actilyse; Boehringer Ingelheim, Ingelheim am Rhein, Germany). The incubation volume was adjusted to the thrombus weight at a v/w ratio of 40 ml g⁻¹. Thrombus weight was measured before lysis and at 10, 30, and 60 minutes after lysis initiation. A 25‐µl aliquot of lysis supernatant was collected at each of these time points for subsequent measurement of D‐dimers by quantitative latex immunoassay (HemosIL D‐dimer HS500, Werfen) on an ACL TOP 700 analyzer (Werfen). At the end of the ex vivo thrombolysis assays, lysis supernatants and residual thrombi were collected and kept at −80°C until analysis.

Desilles J., Solo Nomenjanahary M., Consoli A., Ollivier V., Faille D., Bourrienne M., Hamdani M., Dupont S., Di Meglio L., Escalard S., Maier B., Blanc R., Piotin M., Lapergue B., Ajzenberg N., Vasse M., Mazighi M., Ho‐Tin‐Noé B., Désilles J., Mazighi M., Piotin M., Blanc R., Redjem H., Smajda S., Seners P., Escalard S., Delvoye F., Maier B., Hebert S., Ben Maacha M., Hamdani M., Sabben C., Obadia M., Deschildre C., Lapergue B., Consoli A., Rodesch G., Maria F., Coskun O., Lopez D., Bourcier R., Detraz L., Desal H., Roy M., Clavier D., Marnat G., Gariel F., Lucas L., Sibon I., Eugene F., Vannier S., Ferre J., LeBras A., Raoult H., Paya C., Gauvrit J., Richard S., Gory B., Barbier C., Vivien D., Touze E., Gauberti M., Blaizot G., Ifergan H., Herbreteau D., Bibi R., Janot K., Charron V, & Boulouis G. (2022). Impact of COVID‐19 on thrombus composition and response to thrombolysis: Insights from a monocentric cohort population of COVID‐19 patients with acute ischemic stroke. Journal of Thrombosis and Haemostasis, 20(4), 919-928.

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Measurement of Direct Oral Anticoagulants

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The ACL‐TOP‐700 analyzer (Werfen) was used to measure the prothrombin time (PT, HemosIL ReadiPlasTin) and rivaroxaban or apixaban calibrated anti‐factor Xa assays (HemosIL, Liquid anti‐Xa).¹⁸, ¹⁹ The same analyzer was used to measure plasma drug concentrations for dabigatran using the diluted thrombin time (dTT, HemosIL Direct thrombin inhibitor) calibrated with a dabigatran calibrator²⁰ and the activated partial thromboplastin time (APTT, HemosIL Synthasil). The measurements were carried out following the manufacturer’s instructions. To minimize the risk of laboratory variation, all DOAC measurements were performed on the same day, using reagents from the same batch. Outlying results (i.e., values outside the two times standard deviation [SD] range, or peak values that were only slightly higher than the trough values) were remeasured in duplicate. In addition, creatinine levels were measured (Roche, COBAS 8000 modular) after which the creatinine clearance (CrCl) was estimated using the Cockcroft‐Gault formula.²¹

Toorop M.M., van Rein N., Nierman M.C., Vermaas H.W., Huisman M.V., van der Meer F.J., Cannegieter S.C, & Lijfering W.M. (2021). Inter‐ and intra‐individual concentrations of direct oral anticoagulants: The KIDOAC study. Journal of Thrombosis and Haemostasis, 20(1), 92-103.

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Automated Turbidimetric D-dimer Assay

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HemosIL D-Dimer HS 500 assays (Werfen, Munich, Germany) are fully automated, latex-enhanced turbidimetric immunoassays consisting of a latex reagent in a suspension of polystyrene latex particles with a highly specific binding domain of F(ab')2 antibody fragments (MA-8D3) for D-dimer. The administration of D-dimer in the analyte causes agglutination of the latex particles, which is proportional to the concentration of D-dimer. Agglutination results in turbidity of the suspension, which can be quantified by the transmission of light with a wavelength of 671 nm. The measurement was performed on the ACL TOP 700 analyzer (Werfen, Munich, Germany). Dilution series for linearity assessment were performed using the factor diluent (Werfen, Munich, Germany).

Kohlhase K., Schaefer J.H., Miesbach W., Hintereder G., Kirchmayr K., Zwinge B., Yalachkov Y., Foerch C, & Schaller-Paule M.A. (2022). Measurement of D-dimer in cerebrospinal fluid using a luminescent oxygen channeling immunoassay. Frontiers in Neurology, 13, 951802.

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Validating POC i-STAT Alinity Instrument

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Anonymized whole blood samples were tested on the POC i-STAT Alinity instrument using one lot of cartridges: CG4+, CHEM8+ and PT/INR. The i-STAT Alinity system results were compared to those obtained on existing instruments in central laboratories. The results for pH, Pco₂, Po₂, lactate, sodium, potassium, chlorine, ionized calcium, glucose, hemoglobin and hematocrit were compared to those obtained using the ABL90 Flex Plus blood gas analyzer (Radiometer, Copenhagen, Denmark). The results for urea and creatinine were compared to those obtained by enzymatic methods on a Cobas 8000 instrument using a c702 module (Roche Diagnostic, Meylan, France). The PT and INR measurements were compared to those determined using an ACL TOP 700 analyzer (Werfen, Barcelona, Spain).

Larcher R., Lottelier M., Badiou S., Dupuy A.M., Bargnoux A.S, & Cristol J.P. (2023). Analytical Performances of the Novel i-STAT Alinity Point-of-Care Analyzer. Diagnostics, 13(2), 297.

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