The Hepa1-6 cell line, which expresses high levels of miRNA199a, and HCC cell lines Hep3B, PLC/PRF/5, SKHep1, and SNU423 with miR199a downregulation were obtained from ATCC and maintained in DMEM media (Thermo Fisher Scientific, Scoresby, Australia) supplemented with 10% fetal bovine serum (FBS) (GIBCO, Australia) and 1% penicillin-streptomycin (P/S) (GIBCO, Australia). The Australian Genome Research Facility (AGRF) cell line ID service was used to confirm the identity of the human cell lines. Breast cancer cell lines T47D and MCF-7 were maintained in standard DMEM media. Melanoma cell lines 92.1 and Mel270 (gifted by Nicholas Hayward) and ovarian cancer lines SW626, CAOV3, and TOV21G were grown in RPMI media (Thermo Fisher Scientific) supplemented with 10% FBS and 1% P/S. Prostate cancer cell lines LnCap and DU145 were maintained in standard DMEM media. All the other cell lines were maintained as per the ATCC recommendations. Cryopreserved primary human hepatocytes (HUM4150) and NoSpin HepaRG (NSHPRG) cells were obtained from Lonza (Sydney, Australia) and maintained as per the manufacturer’s protocol.
Hum4150
Manufactured by Lonza
Sourced in Australia
The HUM4150 is a laboratory equipment designed for sample processing and preparation. It is a versatile instrument that can be used for various applications in life science research and development. The core function of the HUM4150 is to efficiently homogenize and disrupt samples, enabling effective extraction and isolation of target analytes or biomolecules. This equipment is suitable for use with a wide range of sample types, including tissues, cells, and other biological materials.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Dmem media, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin p s, by Thermo Fisher Scientific (2 mentions)
Plc prf 5, by American Type Culture Collection (2 mentions)
Hepa1 6 cell line, by American Type Culture Collection (1 mentions)
Rpmi media, by Thermo Fisher Scientific (1 mentions)
Hek293, by American Type Culture Collection (1 mentions)
Tov 21g, by American Type Culture Collection (1 mentions)
Lncap, by American Type Culture Collection (1 mentions)
Cos 7, by American Type Culture Collection (1 mentions)
Hep3b, by American Type Culture Collection (1 mentions)
Sk hep1, by American Type Culture Collection (1 mentions)
Hepa1 6, by American Type Culture Collection (1 mentions)
Snu423, by American Type Culture Collection (1 mentions)
2 protocols using hum4150
1
Cell Line Characterization and Maintenance
2
Cryopreserved Hepatocytes and Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cryopreserved primary human hepatocytes (HUM4150) and HepaRG (NSHPRG) cells were obtained from Lonza, Australia and maintained according to the manufacturer’s protocol. HCC cell lines HuH7 was provided by Dr. Kim Bridle and HCC cell lines Hepa1-6, Hep3B, PLC/PRF/5, SKHep1, and SNU423 were obtained from American Type Culture Collection (ATCC) and cultured in DMEM media (Thermo Fisher Scientific, Scoresby, Australia) supplemented with 10% fetal bovine serum (Gibco, Australia) and 1% penicillin/streptomycin (P/S) (Gibco, Australia). Cell line identification service was provided by the Australian Genome Research Facility (AGRF). Non-HCC cell lines HEK293, TOV-21G, LNCaP, and COS7 were obtained from ATCC and maintained in media mentioned above.
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