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Anti pi4kiiiβ

Manufactured by Merck Group
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Anti-PI4KIIIβ is a laboratory equipment product that functions as an inhibitor of the phosphoinositide 4-kinase III beta (PI4KIIIβ) enzyme. PI4KIIIβ is involved in the regulation of cellular processes such as membrane trafficking and signal transduction. The core function of Anti-PI4KIIIβ is to inhibit the activity of the PI4KIIIβ enzyme, which can be used for research purposes.

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Lab products found in correlation

Anti actin, by Merck Group (2 mentions) Mouse monoclonal anti flag, by Merck Group (2 mentions) Anti acbd3, by Merck Group (2 mentions) Anti scamp3 rabbit polyclonal, by GeneTex (2 mentions) Anti 3d mouse monoclonal, by Merck Group (2 mentions) Anti ns3, by GeneTex (2 mentions) Anti calnexin, by Santa Cruz Biotechnology (2 mentions)

2 protocols using anti pi4kiiiβ

1

Western Blot Analysis of Viral Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins were fractionated by 12% or 15% SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes by the wet method. PVDF membranes were blocked with Tris-buffered saline-0.1% Tween 20 (vol/vol) that contained 5% nonfat dry milk, and were probed with antibodies indicated. Primary antibodies were used at the following dilutions: anti-SCAMP3 rabbit polyclonal, 1:4,000 (GeneTex); anti-FLAG mouse monoclonal, 1:4,000 (Sigma); anti-3D mouse monoclonal, 1:4,000 (a gift from Shin-Ru Shih, Chang Gung University); anti-3A rabbit polyclonal, 1:1,000 (a gift from Jim-Tong Horng, Chang Gung University); anti-PI4KIIIβ, 1:1,000 (Merck Millipore); anti-calnexin 1:500 (Santa Cruz Biotechnology); anti-NS3, 1:5,000 (GeneTex); anti-ACBD3, 1:1,000 (Sigma-Aldrich); and anti-actin, 1:5,000 (Millipore).
Lu J.Y., Brewer G., Li M.L., Lin K.Z., Huang C.C., Yen L.C, & Lin J.Y. (2021). Secretory Carrier Membrane Protein 3 Interacts with 3A Viral Protein of Enterovirus and Participates in Viral Replication. Microbiology Spectrum, 9(1), 10.1128/spectrum.00475-21.
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2

Western Blot Analysis of Viral Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins were fractionated by 12% or 15% SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes by the wet method. PVDF membranes were blocked with Tris-buffered saline-0.1% Tween 20 (vol/vol) that contained 5% nonfat dry milk, and were probed with antibodies indicated. Primary antibodies were used at the following dilutions: anti-SCAMP3 rabbit polyclonal, 1:4,000 (GeneTex); anti-FLAG mouse monoclonal, 1:4,000 (Sigma); anti-3D mouse monoclonal, 1:4,000 (a gift from Shin-Ru Shih, Chang Gung University); anti-3A rabbit polyclonal, 1:1,000 (a gift from Jim-Tong Horng, Chang Gung University); anti-PI4KIIIβ, 1:1,000 (Merck Millipore); anti-calnexin 1:500 (Santa Cruz Biotechnology); anti-NS3, 1:5,000 (GeneTex); anti-ACBD3, 1:1,000 (Sigma-Aldrich); and anti-actin, 1:5,000 (Millipore).
Lu J.Y., Brewer G., Li M.L., Lin K.Z., Huang C.C., Yen L.C, & Lin J.Y. (2021). Secretory Carrier Membrane Protein 3 Interacts with 3A Viral Protein of Enterovirus and Participates in Viral Replication. Microbiology Spectrum, 9(1), 10.1128/spectrum.00475-21.
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