Caspase 8 sc 29930

Gene silencing was achieved by either transfecting siRNA using HiPerFect transfection reagent (Qiagen, Valencia, CA) following the manufacturer's instructions or infecting cells with lentiviruses harboring a given shRNA. Control (i.e., non-silencing) and DR5-specific siRNAs were described previously [35 ]. DR5 shRNA in pLKO.1 (TRCN0000005929) targeting the 3′-untranslated region was purchased from Open Biosystems (Huntsville, AL). ERK1/2 (#6560), JNK (#6234 including #I and #II) and c-Jun (#6205) siRNAs were purchased from Cell Signaling Technology, Inc. TRAF2 (sc-29509), Fra-1 (sc-35405) and caspase-8 (sc-29930) siRNAs were purchased from Santa Cruz Biotechnology, Inc. MMP1 siRNAs that target the sequences of 5′-ACACAAGAGCAAGATGTGGAC-3′ (#1) and 5′-AAGTTGATGCAGTTTTCATGA-3′ (#2) were synthesized by Qiagen (Valencia CA). FADD siRNA was described previously [38 (link)]. Gene silencing effects were evaluated by Western blot analysis as described above.

Gene silencing was achieved by either transfecting siRNA using HiPerFect transfection reagent (Qiagen, Valencia, CA) following the manufacturer’s instructions or infecting cells with lentiviruses harboring a given shRNA. Control (i.e., non-silencing) and DR5-specific siRNAs were described previously [14 (link)]. DR5 shRNA in pLKO.1 (TRCN0000005929) was purchased from Open Biosystems (Huntsville, AL). Caspase-8 (sc-29930) and sphingosine kinase 1 (SphK1; sc-44114) siRNAs were purchased from Santa Cruz Biotechnology, Inc. Gene silencing effects were evaluated by Western blot analysis as described above.