Test compound (2 mg) was suspended with 1 mL of normal saline, pure water, or 5% glucose aqueous solution in glass tube, and the suspension was shaken at 150 rpm for 48 h at 25 °C. An aliquot was filtered by PVDF filter unit (Mini-Uni PrepTM, GE Healthcare), and the filtrate was diluted with pure water. The diluted sample solution was injected into HPLC system (column (Mightysil RP-18, Kanto Chemical Co. Inc.), UV/vis detector (UV-2077, JASCO), pump (PU-2089, JASCO), and column oven (CO-965, JASCO)) and the peak area was recorded at 254 nm. The concentration of solution was calculated with a calibration curve. Examinations of all conditions were performed three or four times.
Mini uni prep
Manufactured by GE Healthcare
Sourced in United States
The Mini-Uni PrepTM is a compact and versatile laboratory equipment designed for sample preparation. It provides a reliable and consistent method for sample processing. The core function of the Mini-Uni PrepTM is to facilitate the preparation of samples for further analysis or experimentation.
Automatically generated - may contain errors
Lab products found in correlation
Co 965, by Jasco (1 mentions)
Uv 2077, by Jasco (1 mentions)
Mightysil rp 18, by Kanto Chemical (1 mentions)
Pu 2089, by Jasco (1 mentions)
Dribulose 1 5 diphosphate carboxylase, by Merck Group (1 mentions)
Yarra 1.8 m sec x150 column, by Phenomenex (1 mentions)
Whatman, by Cytiva (1 mentions)
Mini uniprep, by Cytiva (1 mentions)
2 protocols using mini uni prep
1
Solubility Characterization of Test Compound
2
Rubisco Quantification by SEC-HPLC
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Ultracentrifuged juices were filtered through Mini-UniPrep TM filters with pore size 0.2 μm (Whatman TM , GE Healthcare Life Sciences) before injection into a HPLC (Agilent, United States) with a Yarra 1.8 µm SEC-X150 column (size: 150 x 4.6 mm) (Phenomenex, United States). Four μL of juices were injected into the HPLC and compared to a D-Ribulose 1,5-diphosphate carboxylase (Rubisco) standard from spinach (Sigma-Aldrich, Germany). Protein was eluted with a 0.05 M PO4 buffer with 0.3 M NaCl and pH 6.8 at a flow of 0.3 mL min -1 . UV absorbance was measured at 214 nm. Size exclusion analysis was performed in triplicate.
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