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Anti keratin 10

Manufactured by Santa Cruz Biotechnology
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Anti-keratin-10 is a laboratory product used for the detection and analysis of keratin-10 in biological samples. It is a specific antibody that binds to keratin-10 protein, allowing researchers to identify and quantify the presence of this protein in their experiments.

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Lab products found in correlation

Anti gapdh, by Santa Cruz Biotechnology (3 mentions) Anti chk1, by Cell Signaling Technology (2 mentions) Anti brca1, by Cell Signaling Technology (2 mentions) Anti e2f1, by Cell Signaling Technology (2 mentions) Anti p chk1, by Cell Signaling Technology (2 mentions) Anti chk2, by Cell Signaling Technology (2 mentions) Anti p73, by Abcam (2 mentions) Anti p53, by Merck Group (2 mentions) Anti atr, by Cell Signaling Technology (2 mentions) Anti nbs1, by Cell Signaling Technology (2 mentions) Anti mre11, by Cell Signaling Technology (2 mentions) Anti p chk2, by Cell Signaling Technology (2 mentions) Anti topbp1, by Santa Cruz Biotechnology (2 mentions) Anti p63, by Cell Signaling Technology (2 mentions) Anti brca2, by Cell Signaling Technology (2 mentions) Anti rad51, by Cell Signaling Technology (2 mentions) Anti loricrin, by Santa Cruz Biotechnology (2 mentions) Anti p topbp1, by Abcepta (2 mentions) Anti p atr, by Cell Signaling Technology (2 mentions) Ecl prime, by Cytiva (2 mentions)

3 protocols using anti keratin 10

1

Western Blot Analysis of DNA Damage Response Proteins

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The antibodies from this study were as follows: anti-keratin-10, anti-loricrin, anti-TopBP1, and anti-GAPDH (Santa Cruz, Santa Cruz, CA); anti-E2F1, anti-p63, anti-ATR, anti-p-ATR, anti-CHK1, anti-p-CHK1, anti-CHK2, anti-p-CHK2, anti-RAD51, anti-Mre11, anti-NBS1, anti-BRCA1, and anti-BRCA2 (Cell Signaling, Danvers, MA); anti-p73 (Abcam, Cambridge, MA); anti-p53 (Millipore, Burlington, MA); anti-p-TopBP1 (Abgent, San Diego, CA). AKT inhibitors MK2206 (2μM) and LY294002 (25μM) were purchased from SelleckChem (Houston, TX). Cell lysates were processed and assayed by western blot analysis as previously described 60 (link). Briefly, J2 feeders were firstly removed by Versene (PBS containing 0.5 mM EDTA) treatment and keratinocytes were collected and lysed in RIPA lysis buffer on ice for 30 minutes. The samples were then electrophoresed on SDS-page gels and transferred to PVDF membranes. At last the membranes were developed using ECL prime or ECL reagents (Amersham, Pittsburgh, PA) and chemiluminescence signals were detected using Eastman Kodak x-ray films.
Hong S., Xu J., Li Y., Andrade J., Hoover P., Kaminski P.J, & Laimins L.A. (2019). Topoisomerase IIβ-binding protein 1 activates expression of E2F1 and p73 in HPV positive cells for genome amplification upon epithelial differentiation. Oncogene, 38(17), 3274-3287.
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2

Quantifying Keratin 10 Levels by Western Blot

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Lysate harvesting and western blot analysis was performed as previously described [57 (link)]. To quantify the levels of keratin 10 (K10), insoluble cell pellets obtained from RIPA-SDS lysis were resuspended in 8 M urea, 10% β-mercaptoethanol, 2 mM PMSF, and incubated at room temperature while rotating for 30 min, as described previously [90 (link)]. The insoluble debris was removed by centrifugation at 14,000 rpm at 4’C. Primary antibodies used: anti-SETD2 (Kind gift from Dr. Brian Strahl, Epicypher), anti-H3K36me3, anti-cyclin B, anti-CDK2 (Abcam), anti-H3.1 (Active Motif), p84 (GeneTex), anti-Involucrin, anti-keratin 10, anti-CDC25C, anti-cyclin A, anti-cyclin E and anti-GAPDH (Santa Cruz), anti-CDK1, anti-RPA32 (Bethyl laboratories) and anti-Flag (Sigma). Secondary antibodies used were horseradish peroxidase (HRP)-conjugated anti-rabbit (Cell Signaling Technology) and HRP-conjugated anti-mouse (GE Life Sciences). Western blots were developed using Clarity Western ECL blotting substrate (Bio-Rad). Images were captured on either autoradiography film or Biorad ChemidocMP imaging system. Blots were analyzed with Biorad Imagelab 5.0 software.
Gautam D., Johnson B.A., Mac M, & Moody C.A. (2018). SETD2-dependent H3K36me3 plays a critical role in epigenetic regulation of the HPV31 life cycle. PLoS Pathogens, 14(10), e1007367.
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3

Western Blot Analysis of DNA Damage Response Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
The antibodies from this study were as follows: anti-keratin-10, anti-loricrin, anti-TopBP1, and anti-GAPDH (Santa Cruz, Santa Cruz, CA); anti-E2F1, anti-p63, anti-ATR, anti-p-ATR, anti-CHK1, anti-p-CHK1, anti-CHK2, anti-p-CHK2, anti-RAD51, anti-Mre11, anti-NBS1, anti-BRCA1, and anti-BRCA2 (Cell Signaling, Danvers, MA); anti-p73 (Abcam, Cambridge, MA); anti-p53 (Millipore, Burlington, MA); anti-p-TopBP1 (Abgent, San Diego, CA). AKT inhibitors MK2206 (2μM) and LY294002 (25μM) were purchased from SelleckChem (Houston, TX). Cell lysates were processed and assayed by western blot analysis as previously described 60 (link). Briefly, J2 feeders were firstly removed by Versene (PBS containing 0.5 mM EDTA) treatment and keratinocytes were collected and lysed in RIPA lysis buffer on ice for 30 minutes. The samples were then electrophoresed on SDS-page gels and transferred to PVDF membranes. At last the membranes were developed using ECL prime or ECL reagents (Amersham, Pittsburgh, PA) and chemiluminescence signals were detected using Eastman Kodak x-ray films.
Hong S., Xu J., Li Y., Andrade J., Hoover P., Kaminski P.J, & Laimins L.A. (2019). Topoisomerase IIβ-binding protein 1 activates expression of E2F1 and p73 in HPV positive cells for genome amplification upon epithelial differentiation. Oncogene, 38(17), 3274-3287.
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