3T3–L1 adipocytes with Control KD or Bckdha KD were lysed in ice-cold RIPA buffer with 1x protease inhibitor (Sigma-Aldrich. 30 μg of total protein was separated on 12% SDS-PAGE gel. The proteins were transferred to a nitrocellulose membrane and immunoblotted with rabbit anti-Bckdha (Novus Biologicals NBP1-79616) (1:1000 dilution) and mouse anti-Beta-Actin (Cell Signaling 8H10D10) (1:5,000). Specific signal was detected with horseradish peroxidase-conjugated secondary antibody goat anti-rabbit (1:2,500) or rabbit anti-mouse (1:10,000) using SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific) and developed using Blue Devil Autoradiography film (Genesee Scientific).
Nbp1 79616
Manufactured by Cell Signaling Technology
NBP1-79616 is a laboratory reagent used for various research applications. It is an antibody that can be used to detect and identify specific proteins or molecules within a sample. The core function of this product is to provide researchers with a tool for analyzing and understanding cellular processes and signaling pathways.
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Lab products found in correlation
2 protocols using nbp1 79616
1
Western Blot Analysis of Bckdha in 3T3-L1 Adipocytes
2
Western Blot Analysis of Bckdha in 3T3-L1 Adipocytes
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3T3–L1 adipocytes with Control KD or Bckdha KD were lysed in ice-cold RIPA buffer with 1x protease inhibitor (Sigma-Aldrich. 30 μg of total protein was separated on 12% SDS-PAGE gel. The proteins were transferred to a nitrocellulose membrane and immunoblotted with rabbit anti-Bckdha (Novus Biologicals NBP1-79616) (1:1000 dilution) and mouse anti-Beta-Actin (Cell Signaling 8H10D10) (1:5,000). Specific signal was detected with horseradish peroxidase-conjugated secondary antibody goat anti-rabbit (1:2,500) or rabbit anti-mouse (1:10,000) using SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific) and developed using Blue Devil Autoradiography film (Genesee Scientific).
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